组织细胞

Results: At 3, 7 days post-grafting, SC survived in the lesioned area, hippocampus and ventriculus lateralis. At 14 days post-grafting, SC were migrating along ependyma, meninges and blood vessels. In the area of corpus callosum, SC were arranged like chains. At 60 days post-grafting, the number of SC was decreased and the fluorescent mark of SC was weakened. At 90 days post-grafting, the survival cells were few.

结果：3天、7天时移植的雪旺细胞大量存活于损伤区、海马、侧脑室的脉络丛组织中。14天后可观察到细胞沿软脑膜、室管膜、血管周间隙迁移，并在胼胝体部位排列成索条状。60天时雪旺细胞的标记荧光减弱，细胞密度减少。90天时已观察不到荧光。

Results of HE dye after cerebral alchemic reperfusion showed: Cortex was infiltrated by neutrophil; Cells of corpus striatum and cortex of parietal lobe were stained light; the number off cells obviously reduced; the feature of cells necrosis, such as cells swelling, nuclear fragmentation and lysis et al, would be observed.

HE染色结果显示：脑缺血再灌注后，皮质区中性白细胞浸润，纹状体外侧和顶叶皮质细胞染色淡，细胞数明显 NF－。B在脑缺血再灌注后脑组织局部炎症反应中的作用减少，出现细胞肿胀，胞核碎裂和溶解等细胞坏死特征。

The system was able to help androgone in testis tissue go through meiosis in vitro without addition of testos-terone by providing necessary conditions for differentiation of androgone.Key words: testis;tissue culture;in vitro spermatogenesis;transmission electron microscopy;HE staining

证实，在没有添加睾酮的情况下，此培养体系能够提供睾丸组织块生精细胞分化所需要的条件，使非精子细胞类生精细胞减数分裂为精子细胞。

Results NGF and its mRNA were localized in chondrocytes in the sclera, especially in the binucleate cell. In NGF and its mRNA respectively, the amount of the positive cells in the occluded eyes was significantly higher than that of the control eyes (P.05). In NGF F(subscript DG=20.556, P.001 or in its mRNA F(subscript DG=25.520, P.001, the amount of the positive cells among the occluded groups or the control groups was significantly decreased along with the time.

结果 NGF蛋白及其mRNA均表达於巩膜的软骨细胞中尤其在双核细胞中；两者在各时间段遮盖眼巩膜中阳性细胞数均明显高於对照组(P.05)；免疫组织化学F(下标 DG=20.556 P.01和原位杂交F(下标 DG=25.520 P.01在对照组各组间阳性细胞的表达数目随时间变化明显下降。

It also indicates that the expression of WT1 is regulated strictly in time and space, and the aberrant expression of WT1 may result in retarded differentiation of blastemal cell leading NR or nephroblastoma.

提示在散发性肾母细胞瘤发生中除WT1突变外，WT1表达延迟可能是其中起重要作用的机制之一；WT1基因与肾母细胞瘤的组织分化有关，可能诱导胚基细胞向上皮细胞方向分化。

Methods S-P immunohistochemical staining was used to detect the expression of VEGF-C and its reˉceptor Flt-4in10neighboring noncancerous tissue and60NSCLC cases of non-small cell lung cancer and32lymph nodes cases with lymph nodes metastasis of non-small cell lung cancer.

目的 探讨血管内皮生长因子C及其受体Flt-4在人非小细胞肺癌组织中的表达及其与淋巴结转移等临床病理特征的关系方法采用免疫组化S-P法检测60例NSCLC原发灶组织、10例癌旁组织、32例伴有肺癌转移的淋巴结组织中VEGF-C、Flt-4的表达，并将结果与肺癌的临床病理特征进行分析。

The mechanism of Si Junzi Tang and panax sapogenol were studied on the effect of gastrointestinal cells protection by applying advanced technology of histochemistry, Holt′s cytodieresis mircocirculation, radio-immunity and subcellular ultrastructure.

进而应用酶组织化学、Holt细胞分裂测定、微循环测定、放射免疫测定、亚细胞超微结构观察等先进技术，研究四君子汤、人参皂甙抗胃肠细胞损伤、保护胃肠细胞的作用机理。

It was negative for co-culture of Vero cells with upper fluid of iris, ciliary body and retina which were blindly transfered to the third generation. No Cytopathological effects were observed, while the positive contrast group had CPE including such as ballooning degeneration, karyopyknosis ,multinucleated giant cell even cell necrosis.

Vero 细胞分别与虹膜、睫状体、视网膜组织上清液共培养，并盲传三代结果为阴性，未观察到细胞病理学改变，对照组有阳性表现，表现为Vero 细胞气球样变、核固缩、多核巨细胞甚至细胞悬浮死亡。

It has been found that ectogenic GDNF has nervous protective effec,but ectogenic GDNF cannot enter blood brain barrier.

传统观点认为，星形胶质细胞是神经系统的支持细胞，构成神经组织的网架而起支持作用，与神经元的解剖位置关系紧密，参与中枢神经系统的免疫反应，参与构成血脑屏障，目前研究发现，脑缺血时星形胶质细胞异常活跃，通过合成多种蛋白质、细胞因子和神经营养因子等对神经元起保护作用，对缺血性脑损伤的发展和转归具有重要意义。

On the base of review the research development in tissue culture and genetic transformation, Comparative analyses were made on the callus induction and embryogenetic abilities of anther and non-pollinated ovules of rubber tree. Fluorescence detection was implemented with the different in vitro material of Hevea brasiliensis.

本研究在综述了橡胶树组织培养及遗传转化研究进展的基础上，系统地比较了橡胶花药和未授粉胚珠愈伤组织诱导及体细胞胚胎发生能力，对橡胶树不同离体组织进行荧光背景检测，将在植物胚胎发育时期起重要作用的BBM基因导入橡胶树，对电激法转化游离小孢子细胞以及农杆菌介导花药愈伤组织进行初步探索。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。