组织细胞

The results indicate that the longitudinal nerve fibers in cerebral ganglia and a few of small cells in the surface layer of cerebral ganglia present NOS positive reaction. Abundant NOS positive small cells are in the surface layer of pedal ganglia, and abundant transverse positive nerve fibers in the center of pedal ganglia. A large number of transverse positive nerve fibers are in the center of visceral ganglia; abundant positive small cells and nerve fibers are in two anterior lobes; a few of positive small cells and many encircled positive nerve fibers are in the posterior lobe; a large number of radiate positive nerve fibers are in the lateral lobes.

组织化学显示，存在NOS的部位如下：脑神经节内纵行的神经纤维和表层的少量小细胞；足神经节表层的大量小细胞，中央大量水平分布的神经纤维；脏神经节中部大量水平分布的神经纤维，前叶内大量小细胞和神经纤维，后叶内少量小细胞和许多环行神经纤维，侧叶内大量似放射状分布的神经纤维；脑足和脑脏神经索内的神经纤维。

Results:(1)Among 40 rats, 36 rats were successfully established and the rate of success is 90 percent;(2)All the successfully established models demonstrated polydipsia, polyuria and the body weight was not increased. 6 rats suffered cataract after 3 months, and 4 rats died in 6 months;(3)There was an approximately 61% loss of retinal ganglion cells in the central retina and the thickness of retina thinner under microscope ( P 0.01 ).(4) Electroscope changes include the thinner of retina, disorganization of the membranous disc of the rod cells and the thickness of basal membrane of vessal.(5)In normal group, 1 month dibetes mellitus and 1 month treatmen group, there was no expression of ERK1/ERK2 on the retina tissues. In 3 month diabetes mellitus group, the expression of ERK1/ERK2 was positive.

结果：①40只建模大鼠中36只建模成功，建模成功率为90%；②建模成功的大鼠都表现出多饮、多尿、消瘦、体重不增的表现，有6只大鼠在3个月后出现白内障，有4只大鼠在喂养接近至6个月时死亡；③HE染色光镜下6个月大鼠后极部视网膜节细胞层细胞数明显减少，减少约61%(P.01)，后极部视网膜明显变薄(P.01)；④电镜观察，视网膜变薄，视杆细胞膜盘紊乱，血管基底膜增厚等表现；⑤正常组、糖尿病组和治疗组1个月大鼠视网膜中未见ERK1/ERK2的表达，糖尿病组视网膜组织中3个月时可见少量表达，ERK1/ERK2表达部位为神经节细胞层和内核层；6个月时表达强阳性，部位表达不仅见于内核层、神经节细胞层，色素上皮层也见表达。

The traits of dedifferentiation and redifferentiation of the explants form H. sampsonii show that the calli the stem and leaf usually form buds at its parietal cells. In the presence of auxin, the parenchyma of the leaf gap at the node is initiated firstly, then, develops blastemata that form buds in a cluster on the node without callus. These show that the various parts ofthe herb differ greatly in cell initiation and organ differentiation.

研究元宝草外植体的脱分化和再分化的特点结果表明，茎段和叶片启动形成愈伤组织，其不定芽通常发生在愈伤组织的周边区，茎节叶腋的叶隙薄壁组织在激素的诱导下启动、分裂，不形成愈伤组织而直接器官分化成芽原基，并在节结处产生一圈成簇的不定芽，表明元宝草不同部位的细胞启动及器官分化调控存在差异。

Mechanical environment, as a native survival one, is very complicated and constituted of many different kinds of forces, such as the forces in vitro or between tissues and cells, and the interactive intracellular stress during the process of formation and remodification of cytoskeletons. All these forces regulate bodies' developments and cellular differentiations, which is particularly obvious in bone tissues.

力学环境是自然赋予生命体的一种生存环境，在这个复杂的环境中，生物体外的力、生命运动过程中产生的体内各组织、细胞间的相互作用力及细胞骨架形成和变化时产生的细胞内张力交互作用，构成了一个力学系统，调节着生物体的生长发育和细胞分化，这一点表现在力对骨骼系统的作用上尤为明显。

With western blot analysis and DNA polymerase chain reaction-single strand conformation polymorphism assay,protein product of Rb gene and mutation in exon 5～8 of P_(53) gene were examined in 17 cases of human testicular seminoma.

采用Western印迹和DNA聚合酶链反应－单股构造多态性分析法对17例人睾丸精原细胞瘤组织标本的抗癌基因Rb表达的蛋白产物和P_（53）基因外显子5～8的突变进行检测，发现有3例标本的Rb基因表达的蛋白产物缺失，有4例标本的P_（53）基因有点突变，对照的正常人睾丸组织和视网膜组织均呈Rb基因蛋白产物表达阳性，对照的正常人睾丸组织中未发现P_（53）基因突变。

In this report, immunohistochemistry and in situ hybridization techniques were perfected to examine the expression of bFGF, fibroblast growth factor receptor-I and bFGFmRNA of cultured HEP-2 laryngo-tumor cell and 803 gastro-tumor cell and of the solid tumor tissue formed by implanting the cultured cells into subcutis of nude mice, intending to elucidate the correlation between the expression level and the tumor formation.

本研究建立了免疫组化和原位杂交的检测方法，通过对体外培养的HEP-2喉癌细胞和803胃癌细胞和这两株细胞在裸鼠皮下形成的实体瘤组织中bFGF，纤维细胞生长因子受体-1（fibroblast growth factor receptor-1，FGFR-1）和bFGFmRNA的检测试图揭示三者在体外和体内表达以及与肿瘤形成的关系，从而为这方面的研究提供更多的材料。

The RNA in situ hybridization experiments revealed that MOC1 was initially expressed in a few epidermal or subepidermal cells in the leaf axil prior to the formation of axillary meristem. The expression of MOC1 was continued during the formation process of axillary meristem. MOC1 was still expressed after axillary meristem has differentiated into tiller buds.

MOC1基因的组织原位杂交实验表明，在腋生分生组织形成之前，MOC1基因就已经在叶腋里的少数表皮或亚表皮细胞里表达，并且MOC1基因的表达伴随着腋生分生组织形态建成的整个过程；在腋生分生组织分化形成分蘖芽以后，MOC1仍然在整个分蘖芽中表达。

Some single embryogenic cells produced an embryonic cell and a suspensor cell by the first division and embryonal suspensor masses were produced after several divisions.

胚性愈伤组织中的一些单个胚性细胞经过第一次分裂产生两个细胞，即胚细胞和胚柄细胞，它们继续进行分裂几次以后形成胚性胚柄团结构。

The experiment result showed that the highest content of oleuropein was in the flower,On the basis of more than 300 times of experiments and more than 2000 test samples of data determination in this thesis, we gained the achievements in the following:1. The establishment of callus culture system and the suspension cell culture system of Syringa pubescens Turcz.

建立了小叶丁香的愈伤组织培养体系和悬浮细胞培养体系首次选择小叶丁香的叶片、叶芽和嫩茎等不同部位作为外植体，利用植物组织培养技术，在添加相应的植物生长调节剂的LS、MS等培养基上成功地诱导产生出愈伤组织，其中叶片最容易诱导产生愈伤组织，其次为芽和嫩茎。

To elucidate whether overexpression of Nolz-1 may negatively regulate cell proliferation and cell survival during neurogenesis, we performed immunohistochemistry analysis in the developing telencephalon with specific markers of cycling cells and apoptotic cells.

为了阐明过度表现 Nolz-1在神经发生过程中，是否会负向调控细胞增生及细胞生存，我们利用周期细胞和凋亡细胞专一性抗体，在发育端脑中进免疫组织染色分析。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。