组织细胞

Immunohistochemistry results revealed that AQP8 protein was expressed in placental syncytiotrophoblasts, amniotic epithelial cells, and chorion cytotrophoblasts.

免疫组织化学结果显示，水通道蛋白8表达于胎盘合体滋养细胞、羊膜上皮细胞及平滑绒毛膜细胞滋养细胞。

The nucleus of the transfected cells were crenation or broken down into pieces, which proved that ERIP1 could induce apoptosis.

在免疫组织化学实验中，将带有GFP标签的ERIP1转染入HEK-293T和MCF-7细胞，发现细胞大面积死亡，细胞中转染入质粒后，细胞核皱缩、破碎，说明ERIP1可能诱导细胞凋亡。

Methods Morphology observation, chromosome cytogenetics, tumorigenesis in nude mice, and the sensitivity to virus were examined.

采用细胞形态学观察、细胞遗传学染色体分析、细胞在裸鼠体内恶性生长情况的组织病理学检测以及细胞对病毒的敏感性等方法。

The positive expression of Slit2 located in the cytoplast on oligodendrocyte and astrocyte.The positive cells were found at 3d after spinal cord injury, reached peak on 7d after injury, declining after 14d. The change of Slit2 was correlative with the rehabilitation and regeneration of the axon on the forepart period.

免疫组织化学检测：Slit2蛋白定位于星型胶质细胞和少突胶质细胞的胞浆，呈棕黄色染色，于脊髓损伤后第3 d出现，阳性细胞逐渐增多，于脊髓损伤后第7 d，Slit2阳性表达的细胞数达到高峰并趋于稳定，伤后第14 d开始逐渐下降，呈一过性增高。

They existed as dosage-effect and time-effect. All of these results proved that Sertoli cell was the first target cell, spermatogenic cell was the second one and mitochondrion was the target. Otherwise, we proved this compound can disturb the development and growth of sperms, influence the activity and amount of sperms and increase the deformation ratio.

结合组织病理和性激素及酶活性的改变，揭示该化合物睾丸毒性的靶细胞是支持细胞，其次是生精细胞，作用的靶点是细胞内的线粒体，此外还证明该化合物干扰精子的生长和发育，影响精子的活力和数目，增加畸形率，是雄性精子畸变的诱导剂。

Methods: We divide the rats into 5 groups in random, with Morris method, detect the change of some active oxygen radicals such as super-oxide demitasse, Nitric Oxide Synthase, malondialdehyde etc. Also, we detect the change of cholinesterase, Monoamine Oxidize; using HE,PAS staining, immuno-histochemical technique, detect hippocampus CA1 cerebral pathology slices optical density value of NOS, CHE and lipofuscin of neural cell; detecting apoptosis rate and the expression of P53 , which are neuronal apoptosis related genes.

大鼠随机分为5组，采用"Morris水迷宫"法观察脑缺血性模型大鼠学习记忆功能，检测超氧化物歧化酶、一氧化氮合酶、丙二醛等自由基代谢异常的改变，检测胆碱酯酶、单胺氧化酶等老化相关酶的变化、脑组织神经细胞免疫组织化学技术、检查海马CA1区脑组织的病理切片，检测NOS、CHE光密度值；流式细胞技术：检测细胞凋亡率，及促细胞凋亡基因、P_(53)蛋白表达；病理切片采用HE染色、PAS染色及免疫组化法检查，检测对大鼠海马神经元细胞一般病理变化及脑细胞内脂褐含量。

The reasons of RA focus on disbalance of immunity net, the effect of so many cytokines and MMPs , and the tumor-like proliferation of fibroblast-like synovicytes in joints, though the cause of developing disease is unknow.

RA的发病过程中，免疫网络失衡，加上成纤维样滑膜细胞(fibroblast-like synoviocytes，FLS)的类肿瘤样生长及所分泌的大量促炎性细胞因子、趋化因子和金属蛋白酶，是导致关节内炎性细胞聚集，滑膜细胞增生和向周围组织侵蚀及基质降解和破坏的主要因素。

At 8 weeks postoperatively, the grafts were mostly substituted by new cells, and the artificial ligament was completely parceled by the synovial membrane, but there were still some gaps; The combined tendon exhibited no loose or disfiguration, the infiltrative inflammatory cells disappeared. The cells in grafts were close to normal cells in anterior cruciate ligament, but fibrocytes arranged in disorder and fibers showed a verticality.

移植后第8周，移植物大部分组织被新长入的细胞替代，人工韧带被滑膜完全包裹，浸润的炎症细胞基本消失，部分接触面已融合，但部分面仍有间隙；联合肌腱无变形和松弛，移植物内细胞形态，数量接近正常前交叉韧带，但纤维细胞排列杂乱，纤维有了一定的纵向性。

Most aneurysms specimens showed structure changes, including injury of endothelial cell, disrupture or disappearance of the internal elasticlayer, scarce of contractile fiber cells.

动脉瘤瘤壁中有不同程度的单个核细胞成分的浸润(淋巴细胞或单核/巨噬细胞样细胞)，尤其在"中膜"层或"中膜"与内膜交界处较为明显，同时，在细胞集中区伴有数量不等的新生的营养血管，以上改变类似"肉芽组织"样改变。

Preadipocytes from epiploon of newborn calf were cultured by monolayer adherent method, and total RNA of the preadipocytes was extracted on day 4,6,8,10,12,14 and 16 post-culture, respec-tively. The abundance of ADPN mRNA and HSL mRNA was detected by real-time fluorescent quanti-tative RT-PCR during differentiation period from preadipocyte to adipocyte.

选取犊牛大网膜脂肪组织，进行牛前脂肪细胞的单层贴壁培养，在细胞接种后第4、6、8、10、12、14、16d分别提取细胞总RNA，采用实时荧光定量RT-PCR方法，检测了体外培养的犊牛前脂肪细胞不同时期ADPN基因mRNA和HSL基因mRNA的表达情况。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！