组织细胞

Under optical microscope, the cell of NS arrayed as nest, the nuclear was big, deep-stained, thickened-karyotheca, chromatin granulated, Pathologic karyokinesis can be seen.

DDP组癌巢周围可见少量纤维组织增生、局灶性坏死，并可见炎细胞浸润；LNT组镜下见少量细胞凋亡，凋亡细胞变小，变圆，核固缩，胞浆浓缩，染色加深，凋亡细胞周围未见炎症反应；LNT+DDP组与LNT组镜下大体相同，但可见到较多量的凋亡细胞。

Each group with drug had effect on inhibiting Lewis lung cancer tumor,LNT DDP had the best effect,46.2%,the rate of inhibitory effect of LNT、DDP was 35.6%、39.7%respectively.3.Under optical microscope,the cell of NS arrayed as nest,the nuclear was big,deep-stained,thickened-karyotheca,chromatin granulated,Pathologic karyokinesis can be seen.

DDP组癌巢周围可见少量纤维组织增生、局灶性坏死，并可见炎细胞浸润；LNT组镜下见少量细胞凋亡，凋亡细胞变小，变圆，核固缩，胞浆浓缩，染色加深，凋亡细胞周围未见炎症反应；LNT DDP组与LNT组镜下大体相同，但可见到较多量的凋亡细胞。

He type of ossein in group B was mainly type Ⅰ collagen while that in group A type Ⅱ and it exhibited as a process of fiber formation in group C. The reason for why TGF-β〓 can promote ossification induced by BMP is that can promote the differentiation and proliferation of mesenchymal cells to supply more target cells for BMP, promote the differentiation and proliferation of mature osteocytes and chondrocytes, inhibit metalloproteinase to decrease the destruction of bone tissue and promote calcification of bone matrix.

GF-〓能促进BMP骨诱导作用主要因其能促进间充质细胞的分裂增殖为BMP提供丰富的靶细胞、促进成熟骨及软骨细胞的分裂增殖、促进骨细胞产生Ⅰ型胶原而抑制软骨细胞产生Ⅱ型胶原，抑制金属蛋白酶的活性以减少组织破坏、促进基质钙化等作用而使骨生成能力增加，提示它和BMP具有协同作用。

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

Histological and cellular research on rachis of healthy and inoculated spikes of the mutants at the different stages showed that, structure and developed tendency of cortical sclerenchyma tissue and green tissue and fibrovascular tissue in the rachis were alike between the mutants and their donors at anthesis stage, which also showed no apparent difference in the inoculated spikes'rachis between the mutants and their donors, major difference was that the mutants had less hyphae number than their donors.

对抗赤霉突变体的健康穗及接种穗的穗轴细胞组织学研究结果显示，抗性突变体与其感病亲本的花期健康穗轴在皮层厚壁组织、绿色组织及维管组织结构上基本一致，不同发育时间的变化趋势也一致；接种处理后，抗性突变体与其感病亲本的穗轴组织在各方面的变化趋势相一致，变化量也无明显差异。

But the chondrocytes lost primary characteristics after several passages.Conclusion A great of chondrocytes can be got from xiphoid, and the chondrocytes can grow in suspension and keep primary characteristics for a long time when they are cultured with polyHEMA in vitro. In this way, good seed cells are provided for experiment in vitro, and for the study of tissue engineering, and for clinical study.

从剑突可获得大量软骨细胞，使用PolyHEMA试剂体外培养的软骨细胞，其软骨细胞能长期以悬浮状态生长，并能使软骨细胞长期保持原有的生物学特性，为体外实验及组织工程学的研究提供更优质种子细胞，为临床的进一步研究提供理论基础。

The quantitive value of DNA was 1.82% and 1.92% respectively in nonembryonic cells and the embryonic; during bicellular stage, quadracellular stage and multicellular stage, content of DNA increase slowly, and reach the peak at pear-shap ed embryonic stage keep high level at mature embryonic stage.

非胚性细胞与胚性细胞期的量化值分别为1.82%和1.91%；在二细胞胚、四细胞胚、多细胞胚时期DNA缓慢增长，随着胚性愈伤组织的发育，DNA的含量在梨形胚时期达到高峰；成熟胚的DNA含量虽有所下降，但仍维持较高水平。

The quantitive value of DNA was 1.82%and 1.92%respectively in nonembryonic cells and the embryonic; during bicellular stage, quadracellular stage and multicellular stage, content of DNA increase slowly, and reach the peak at pear-shaped embryonic stage keep high level at mature embryonic stage.

非胚性细胞与胚性细胞期的量化值分别为1.82%和1.91%；在二细胞胚、四细胞胚、多细胞胚时期DNA缓慢增长，随着胚性愈伤组织的发育，DNA的含量在梨形胚时期达到高峰；成熟胚的DNA含量虽有所下降，但仍维持较高水平。

Using acupotomology incise and separate the hip joint articular capsule and ligament and the periosteum on the collum ossis femoris and the soft tissue nearby the joint can cause slight hurt and haemorrhage. The hurt factor is release from the local tissue. Human self-rebuilding system is activated, Building bone function of osteoblast is accelerated, new bone is built quickly. New blood vessel and nerve ending is rebirth. Local tissue's blood supply and nerve nourishment is improved. The developing of bone necrosis is prevented. The necrotic part is re-blood vessel. The conditions of the necrotic's absorbing tissue and the new bone's replacing are established.

针刀治疗股骨头无菌性坏死是通过针刀对髋关节囊、关节囊韧带、股骨颈的骨膜和髋关节相邻周遍组织切割分离铲拨松解可以产生轻微创伤、出血、局部组织释放创伤因子，激发人体自我修复重建反应，激活成骨细胞造骨功能，加速新骨生成，促进新血管和神经终端组织再生与重建，使局部组织血流加速，改善血液供应和神经营养状况，阻止坏死进一步发展，使股骨头颈坏死区域再血管化，为坏死组织吸收和为新骨再生爬行替代过程创造条件。

Then the cell proliferation activity was detected using MTT colorimetrics, the cell cycle and apoptosis after LPS stimulation using flow cytometry, and expressions of caspase3, Bcl2, Bax using immunocytochemical staining.

mg/L的LPS作用于细胞，采用MTT染色法检测LPS对细胞增殖的影响；采用流式细胞术检测细胞周期及凋亡；采用免疫组织化学染色技术检测细胞的caspase3、Bcl2、Bax表达变化。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！