组织细胞

Japonicus and analyzed the role of animal process of oocyte during ovulation and oocyte maturation. What's more, we studied the role of microtubules and microtubule organizing center upon GV migration. The oocyte of A. japonicus connects to the follicle via animal pole process. When placed in normal sea water, ovulation started spontaneously.

利用DTT诱导刺参卵母细胞成熟，综合运用光镜、电子显微镜及激光共聚焦技术观察卵母细胞成熟过程，分析了卵母细胞表面动物极突起在卵母细胞脱滤泡中的作用，以及卵母细胞动物极突起和微管组织在生发泡移动过程中所起的作用。

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

Quantitative chromatics analysis of large bowel tumorous adenoma and adenocarci-noma was made by the methods of computer image processing.

本文通过对大肠腺瘤和腺癌组织进行计算机图像定量分析，表明大肠腺瘤和腺癌的腺上皮细胞以正常杯状细胞和柱状细胞的色度学特征分布为基准可划分为两大类：杯状细胞性腺上皮和柱状细胞性腺上皮。

ResultsYAG laser could healing the pigment pathological changes, chromatophore present vacuolus.But the Vallate frame remaining Integrity.The surrounding tissue of chromatophore have not injury and still survival. Macrophage was heightened, pigment granule appear in the cell.

结果：YAG激光治疗色素病变可以消失，色素细胞呈现空泡状，但色素细胞轮廓的框架仍可完整保留，色素细胞周围组织并未受损伤仍可存活，并可见巨噬细胞增多，该细胞内出现色素颗粒。

Results Osteoblasts from human bone marrow were successfully induced and differentiated; the cuttlebones were reshaped in cuttlebone hydroxyapatite for the first time; and some tissue engineering bones, such as the osteoblasts/...

结果 人骨髓细胞培养中诱导、分化出成骨细胞；首次将乌贼骨改性为乌贼骨羟基磷灰石；构建出成骨细胞/ CHA5 0 0 R、成骨细胞/乌贼骨羟基磷灰石、成骨细胞/聚羟基丁酸酯等组织工程骨。

Myconrrhizal fungus infected the cortical cells and formed pelotons in the cells, after it penetrated the exodermal passage cells through velamen cells. It is interesting that strain AR-18 also penetrated exodermis from the cortical cell next to it, where mycelium grew vigorously and infected inner cortical cell, even a few mycelium penetrated endodermis while there was no mycelium in the fibrovascular bundles.

真菌AR-15与金钗石斛一起培养时，真菌菌丝与根被细胞密切接触并侵入根被细胞，也可通过侵入根毛进入根被细胞，由根被继续侵入外皮层通道细胞，进一步向皮层细胞侵染；部分根被真菌危害，根部组织内有大量真菌孢子和菌丝。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生；HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润，纤维细胞轻度增生，硬膜外神经根内有炎细胞浸润及炎性渗出；特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变；免疫组化染色，给药后四周及八周时，硬膜内外均有Ⅱ型胶原纤维增生；固兰染色B、C、D三组未见有明显脱髓鞘改变，与A组相比无明显异常改变；电镜观察B、C、D三组在给药后的四周及八周时，表现为硬膜外神经根内髓鞘厚薄不一，形状不规则，可见成纤维细胞，神经内膜中可见有巨噬细胞；粗大的有髓神经纤维变化明显，无髓神经纤维受累较轻；八周时电镜下D组改变较B、C两组为轻。

Through using the hot effect of covering, 60 various microelements and mineral elements are absorbed by the skin, which can prevent the cell from aging, promote the cell regenerate and metabolism of the epidermis horniness cell, as well as reduce fat and slim body.

利用包裹的热效应；60多种微量元素和矿物质敷泥膜时被皮肤吸收，可以防止细胞组织老化，促进细胞再生，表皮角质细胞的代谢，达到降脂瘦身的作用，还有作用于细胞内的酶，赋予细胞活力，肌肤富于弹性。

During the course of culture in vitro, the biological function of human nasal septal cartilage gradually degrade in accordance with increasing times of passage, chondrocytes after the third generation are not suitable for human cartilage tissue engineering seeds or studying cytobiology.

人鼻中隔软骨细胞在体外培养过程中，随传代次数的增加而功能活性逐渐降低，第3代以后的细胞不适于作为人软骨组织工程种子细胞和用于细胞生物学等方面研究关键词：葡萄糖氨基浆糖类；软骨细胞；细胞外基质；生物材料

Results 1、 Generally, we can see the original blue and white, shiny, no cracks in the articular surface of the cartilage after the stress increases gradually yellow, surface roughness, cracks appear; when the pressure decreases, the yellowing, rough, the color of the fracture restore gradually and become shiny.2、the shiny smooth surface can be seen under a light microscope, formation, cell distribution, tidy, clear the level of cartilage at the articular surface stress increases, the surface roughness changes, defects, disordered cells, uneven dyeing ; when the articular surface of the pressure gradually decreased, the cartilage gradually repair and the surface of cells at the surface appear only disorder.3、immunohistochemical observation can be seen throughout the observation period, cartilage cells are type Ⅱ collagen expression and expression after 3 weeks gradually weakening, when the seventh week begin to strong gradually.4、 electron microscopy shows that when stress increases the articular surface, the cartilage cells became flat, the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders; and when stress decreases the articular surface, cartilage cells gradually returned normal, cytoplasm in the endoplasmic reticulum, Golgi body gradually restore quantity; collagen fibers with a gradual rules.

结果：①大体观察可见到原本蓝白色、有光泽、无裂纹的软骨在关节面压力增大后，逐渐呈灰黄色，表面粗糙，出现裂隙；当压力逐渐减小后，变黄、粗糙、有裂隙的软骨颜色逐渐恢复，变得有光泽②光镜下可见表面光滑、平整，细胞分布均匀、整齐，层次清楚的软骨在关节面压力增大后，表面变粗糙、缺损，细胞排列紊乱、染色不均；当关节面压力逐渐减小后，软骨表面逐渐修复，细胞仅在表层排列紊乱③免疫组织化学观察可见整个观察期内软骨细胞胞浆内均有Ⅱ型胶原表达，术后3周内表达逐渐变弱，从第7周时开始逐渐变强。④电镜下可见当关节面压力增大后，软骨细胞逐渐变扁，胞质中内质网膜、高尔基体减少，胶原排列紊乱；当关节面压力减小，软骨细胞形态逐渐恢复正常，胞质中内质网膜、高尔基体数量逐渐恢复；胶原纤维排列逐渐有规则。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。