组织细胞

Except on the testis tissues,the expression of ZFY gene were found on the kidney tissues of human embryo or rat, brain vesicles〔rat embryo 13 days〕,and sclerotome cells〔rat embryo 13 days〕 It is possible that because kidney and genital system come from same origin,ZFY gene expressed on the kidney tissues too, the expressive function of ZFY gene on the embryonic brain vesicles and sclerotome cells is not yet clear.

除了在睾丸组织上表达外，ZFY基因还在肾脏组织及鼠胚脑泡(胚胎13天)，生骨节细胞(胚胎13天)上表达。在肾组织上表达可能是肾脏与生殖系统起源相同有关。在胚脑泡及生骨节细胞上表达其作用不清楚。

P＜0.01. 28d, q=4. 0452, p＜0.05 . Corresponding pathologic result shows that the corneal stromal of height edema increase thickness to have infiltration of inflammation cells with hyperplasia of fibroblast cell and large vessel. The collagen fiber in stromatic layer is dissolved and destroyed seriously, suggesting that in addition to HSV-1's direct destructuring effect, inflammatory cells that might play an important role in the pathology of HSK induce the immune-pathological reaction.

相应的组织病理学研究结果显示（UV-B 14天）：病毒对照组角膜基质层高度水肿、增厚，并有大量中性粒细胞等炎性细胞浸润、成纤维细胞和新生血管增生，胶原纤维肿胀、分解等，说明除HSV-1对角膜组织直接破坏外，炎性细胞介导的免疫病理反应在复发性HSK发病机理是重要的因素。

Recently ICAM-1 has also been shown to be the cellular receptor for the major group of rhinoviruses and subtertian malaria infected red cell.

目的：检测细胞间粘附分子-1(ICAM-1)在正常肝组织和肝脏肿瘤组织及细胞中的表达情况，进行人细胞间粘附分子-1的cDNA克隆及建立稳定的转染细胞株。

The changes in the weight, exponential, structure of the tissue, cell apoptosis in testis and epididymis and the changes in the sperm tectology in epididymis were measured on the day 8, 13, 21 and 35 of the treatment,respectively,by the method of morphological examination.

分别在热应激持续到8d、13d、21d和35d时，应用形态学观察法，研究受试鼠睾丸和附睾重量及指数、组织结构、细胞凋亡和附睾精子形态学的变化；应用免疫荧光细胞化学和蛋白质印迹分析的方法，研究睾丸和附睾组织中热休克蛋白70(HSP70)的表达；应用比色法检测睾丸和附睾组织内活性氧和丙二醛、抗氧化物酶(SOD、GSH-Px、CAT)的活力或浓度的变化；应用交配试验，检测雄鼠受精能力及其致孕雌鼠的产仔数的变化，用以揭示热应激对雄鼠生殖机能的影响。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中，雏鹅临床表现为高发病率和高死亡率，食欲和饮水减少，拉白色、黄绿色稀粪，伴有神经症状；常规组织学观察，脾脏和胰腺肿大，有白色坏死斑点，肠道出血和坏死，肝脏肿大变性，肾脏肿大变性有尿酸盐沉着等；用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究，结果表明：胰腺中发现病毒包涵体，肠道中大量纤维素以及胶原纤维增生，肾脏、肺脏大量含铁血黄素沉着，免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少；电镜下多种细胞呈现线粒体水肿，多种实质细胞的染色质固缩，染色质边集，特别是细胞内容物如脂肪滴增多的病理变化特征。

ZHAO Yede*,HE Qinglian.Department of Plastic Surgery, Navy 411 Hospital, Shanghai 200081Objective To investigate the expression level and intracellular distribution of calreticulin in hypertrophic scar-derived fibroblastsand normal skin-derived fibroblasts.

增生性瘢痕(Hypertrophic scar， HS)作创伤及外科手术后常见且十分棘手的皮肤组织疾病，瘢痕组织内成纤维细胞异常活化和细胞外间质过度沉积既是HS的重要组织学特征，也是HS发生、发展的物质基础。

The coil spring were kept constant and recorded for 0hr, 1 hr, 2 hrs, 4 hrs, 8 hrs, 12hrs, ldays, 3days or 7days. Upon completion of experiments, the maxillae were removed. The specimens were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer for 24 hrs and decalcified in 10% ethylene diamine tetraacetic acid at room temperature for 5 wks.

本研究通过建立正畸大鼠牙齿移动动物模型和体外细胞培养，结合细胞力学试验方法和分子生物学测试技术，拟从组织、细胞水平以及分子生物学角度探讨ATF4在正畸牙齿移动过程中对牙周组织改建的影响及作用机制，进一步为正畸牙齿移动提供理论和实践依据。

Results For callus induction of leaf explants of C. orchioides, dark treatment gave better results compared to light treatment; among the media tested, the suitable phytohormone combinations were 2.0 mg/L 2, 4-D or 6-BA 1.5 mg/L 2, 4-D 2.5 mg/L, and 300 mg/L CH 0.2% AC was good for plantlet regeneration from leaf explants. The callus from leaf explants mainly originated from midrib. The parenchyma cells near epicuticle of midrib firstly were initiated to division. Then the parenchyma cells of vascular bundle sheath and mesophyll cells on each side of vascular bundle were also divided to form callus.

结果对仙茅叶片的愈伤诱导，黑暗的效果好于光照；在所试验的培养基成分中，适宜的激素配比是2.0mg/L2，4-D或6-BA1.5mg/L 2，4-D2.5mg/L，并且附加300mg/L水解酪蛋白和0.2%活性炭，对于仙茅叶片的离体成苗较好；培养后，愈伤组织主要由叶片的中脉产生，位于中脉上表皮内侧的薄壁细胞首先启动分裂，随后维管束鞘薄壁细胞及其外侧的叶肉细胞也启动分裂，参与愈伤组织的形成。

Methods The Fas, FasL and DR4, DR5, TRAIL were assessed in 45 normal cervices, 52 cervical intraepithelial neoplasia gradeⅢ and 51 cervical cancers by immunohistochemical method. The number of apoptotic cells was determined by morphological criteria and the number of proliferating cells by counting Ki-67-positive cells. Results Proliferation and apoptosis percentage was markedly increased with increasing severity of neoplasia.

应用免疫组织化学法检测45例正常宫颈组织、52例宫颈上皮内瘤变Ⅲ级(cervical interaepithelial neoplasia Ⅲ， CINⅢ)和51例宫颈鳞状上皮细胞癌组织中Fas、FasL、DR4、DR5、TRAIL的表达，并以显微镜定位表达位置，以Ki-67阳性细胞记数增生细胞数。

Compared with the control, the lungs of 3-day-hyperxia-exposure group developed into edema, hemorrhage and inflammatory infiltration and this condition aggravated at the 7th day. The pulmonary septum was significantly widened at the 14th day, the interstitial fibrocytes as well as alveolar epithelial cells proliferated obviously, and the tendency of lung fibrosis was also presented. The results mentioned above showed that the modle of hyperoxia lung injury was successfully established, and the acute inflamationm was its early symptom while the fibrosis was its late manifistation. There was no positive cytes of IFN-γ in the lung of nomal rats, The positive cytes of the groups under hyperxia-exposure for 3, 7, 14 day, which climbed to the climax at the 7th day and began to decline from the 14th day, were found in alveolus epithlia,pulmonary interstitial, bronchoepithlia and macrophage.

高氧14天（5.46±0.30）虽有所下降，但同空气组比，差异仍然具有显著性（P.05）；BALF中蛋白含量高氧3天（0.45±0.10）、7天（0.66±0.08）、14天（0.79±0.14）显著高于空气组（0.18±0.08，P值均。05）；肺通透系数高氧3天（6.61±2.05）、7天（10.88±1.54）、14天（12.75±2.20）均明显高于空气组（2.99±1.27，P值均。05）；同时病理组织学改变也表明与空气组比较，高氧3天时肺组织水肿、出血、炎性细胞浸润，7天时进一步加重，14天时肺间隔明显增宽，间质纤维细胞和肺泡上皮细胞明显增生，出现肺纤维化倾向；以上结果均表明我们成功地建立了高氧肺损伤动物模型，且急性炎症反应是其早期表现，纤维化改变是其晚期表现。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。