组织细胞

Expressions of ChAT mRNA and protein were enhanced in 9 of the 18 lung tumor specimens; and expressions of ChoK mRNA and protein were increased in 14 of 18 lung tumor specimens compared with those of normal lung tissues. 8 of 18 lung cancer specimens were obvious increased in expressions of both ChoK and ChAT.

结果 18例肺癌的PET/CT图像均显示胆碱放射性摄取明显增高，18例肺癌组织细胞中，14例Chok mRNA及蛋白质的表达比正常肺组织升高，9例肺癌组织细胞ChAT mRNA及蛋白质表达升高，其中8例Chok及ChAT表达均升高。

Beijingensis under three droughttreatments, normal, moderate and severe stresses. The results showed:(1) the canker disease ofboth cultivars was serious gradually with increased drought;(2) the bark tissue cells sufferedplasmolysis, more evident with the severity of drought stress;(3) under the drought andinoculation with B. dothidea, cells of two cultivars damaged at different degree, mailyrepresented in the changes of organelles, such as chloroplast swollen and distorted, number ofmitochondria increased and membrane system indistinct; then organelles suffered furtherdamagement with inoculation time, thinned mitochondrias stroma, decreased cristae, crumpledand partly broken membrane of chloroplasts with stroma exosmosis. At last, the chloroplastspartly disorganized;(4) the hyphae growed mainly intercellular in resistant cultivar and notonly intercellular but also intracellular in susceptible cultivar, which directly caused thenecrosis of cells;(5) under the severe drought, the damage of cells enhanced the infection ofpathogen and drought and pathogen stressed together and promoted the disease development;the damage from pathogen on cells was more serious than that from drought.

结果显示：(1)随着干旱胁迫程度的增加，2种杨树溃疡病害发生渐趋严重；(2)干旱胁迫下，杨树树皮组织细胞发生质壁分离，并随胁迫程度的增加而严重；(3)干旱胁迫下接种病原菌，2种杨树细胞发生不同程度的损伤，主要表现为细胞器发生较大变化，如出现叶绿体肿胀变形、线粒体数量增多，质膜模糊不清等现象；随接种时间的延长，细胞器受到进一步损伤，叶绿体被膜折皱，严重时局部破裂，基质外渗，并部分最终解体；(4)毛白杨中的菌丝主要在细胞间隙中穿行，而北京杨的菌丝除在细胞间隙中生长之外，侵入细胞内部也较多，直接导致细胞的解体；(5)干旱胁迫下细胞的损伤促进了病原菌的侵染，干旱和病原菌的双重胁迫加剧了病害的发生程度，并且病原菌侵染对细胞的破坏程度大于水分胁迫。

CT displayed the structure of femoral head was vague. A piece of coloboma could be observed clearly on the cartilage of a femoral head. Medullary canal was almost filled with white adipose tissue. Osteoblasts and osteocytes decreased. Empty bone lacuna increased. Thrombus distributed widely in the bone tissue microcirculation.

MA组病理检查肉眼可见股骨头软骨面局部缺损，坏死面暴露，髓腔内大量脂肪组织堆积；光镜下成骨细胞、骨细胞均减少，空骨陷窝增多，微小血栓广泛分布，变形扩大的脂肪细胞挤压正常骨髓组织细胞，骨小梁变细破裂明显。

Therefore, this study would calculate and survey the following indexes as the quantitative indexes for evaluating the degrees of cell senescence in hepatic and brain tissues, including numbers of neurons and dendrites in brain tissues; numbers of normal hepatocytes, dikaryotic hepatocytes, swollen hepatocytes as well as flake necrosis.

为此，本研究将计测脑组织神经元与树突数量；正常肝细胞，双核肝细胞、肿胀肝细胞数目、肝组织小片坏死个数作为评价脑神经及肝组织细胞衰老程度的量化指标。

Furthermore,the changes of cell organelles realized different cell functions, which fiber for mechanical support,parenchyma cell for preservation and vessel for transportation.3.FE-SEM and DCR technique revealed the arrangements of CMfs on cell wall of fiber, parenchyma cell and vessel.Firstly,fiber cell formed multilamellate concentric structure with thin and thick lamellae alternate.

纤维、基本薄壁组织细胞、导管分子的终端分化就是PCD；并且PCD过程是一个具有较长周期的过程，周期长短比较为基本薄壁组织细胞＞纤维＞导管分子；三类细胞最后的凋亡决定于细胞内原生质体完全降解的时刻。

The list of possible causes of diabetes insipidus in this case and many others is extensive and includes infection (tuberculous pachymeningitis and Whipple's disease), malignant neoplasms (germ-cell tumor and lymphoma), infiltrative processes (hemochromatosis and amyloidosis), autoimmune diseases (Wegener's granulomatosis, lymphocytic infundibulitis, and sarcoidosis), and histiocytosis (Langerhans'-cell histiocytosis and non-Langerhans'-cell histiocytosis).

这例病例其他许多病例尿崩症的可能病因广泛，包括感染(结核性脑脊髓膜炎和Whipple病)，恶性肿瘤，变性疾病，自身免疫病（Wegener肉芽肿，垂体茎淋巴细胞瘤和粒细胞肉瘤）和组织细胞增生症（Langerhans细胞和非Langerhans细胞组织细胞增生症

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Results VEGF-C and VEGFR-3 are all expressed on both mesenchymal cells and vascular endothelium of the embryos during early period. In middle and latter period, VEGFR-3mRNA is mainly expressed on cell plasm of alimentary canal, but it has no expression on vascular and lymphatic endothelium; The expression of VEGFR-3 is extensive and positive expression is seen on the vascular and lymphatic endothelium.

在胚胎中、后期消化道组织中VEGF-C主要表达于消化管壁组织细胞以及肠系膜内，在血管和淋巴管内皮细胞则无表达；VEGFR-3亦表现为广泛的表达，于血管和淋巴管内皮细胞可见阳性表达，并随着胚胎的发育，其在淋巴管内皮细胞的阳性表达率明显增加。

Observations from the paraffin dissection showed that, the anatomical structures of treated upland rice root tip cells changed obviously, such as the arrangement confusion of cells from apical root meristem, quiescent center and root cap, increased middle lamella, the increased width and reduced elongation of cortex parenchyma cells, and the large numbers of slough off epidermis cells.

石蜡切片的结果表明，和对照相比，化感物质处理后的旱稻幼苗根尖的解剖结构发生了明显的变化，主要表现在顶端分生组织、静止中心和根冠的细胞排列混乱，细胞间隙加大，皮层薄壁组织细胞变得短而粗，以及表皮层细胞大量脱落等。

Second is studying the traumatic condition changes in a choosey pressure at different post-PT phases. Moreover, we detected model PLA_2 activity, express of PLA_2 subtype mRNA by fluorescent quantitative RT-PCR technique, protein express and site by Western blot and IHC at different post-PT phases. At last, we studied the PLA_2 inhibited effects and model protection of Variabilin in vivo.

研究在不同压强的外力作用下，模型指定时相点的一般情况和存活率、大体、光镜及电镜下胰腺组织的病理变化和常规酶学变化，得到稳定建模致伤压强；然后，固定致伤压强，研究胰腺创伤后不同时相点的伤情变化(包括一般情况、胰腺组织的病理变化、AMS和LPS活性变化、血常规、血生化、胰腺细胞凋亡以及胰腺组织细胞生长周期变化情况)；再次，采用3H-花生四烯酸标记大肠杆菌膜作为底物的方法检测大鼠胰腺创伤后不同时相点的PLA_2活性，利用荧光定量RT-PCR技术检测其亚型mRNA的表达，利用Western blot和IHC技术检测其蛋白表达量和蛋白组织定位；最后，将Variabilin体内应用于急性胰腺创伤大鼠模型，研究其对模型的保护作用和对PLA_2的抑制效应。

It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为！