组织方法

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

Using immunohistochemical and eosin stain, expression of ERK1/2 and Elk in the lung of asthma model was observed.

三、应用免疫组织化学和伊红复染方法，观察了在体哮喘模型肺内ERK1/2和Elk的表达。

Method］reversed sural neurofasciocutaneous flap with muscle was applied for repairing the soft tissue defect simultaneous open fracture of tibia lower section 6 cases,the soft tissue defect simultaneous chronic osteomyelitis of tibia lower section 5 case,the soft tissue defect of sole of foot simultaneous calcaneus epicarp 3 case, the soft tissue defect of sole of foot simultaneous calcaneus osteomyelitis or dead space 2 case. the scope of the flap was 18 c m×13 cm～11 c m×7 c m and that of the gastrocnemius flap was 4 cm×3 cm～9 c m×6 cm,the thickness was 1～3 cm,the skin flap was bigger than the muscle flap.

方法］自2004年6月以来，应用带肌肉的腓动脉穿支蒂腓肠神经营养血管皮瓣修复小腿下段及足踝部软组织缺损16例，其中胫骨下段开放性骨折伴软组织缺损6例，胫骨下段软组织缺损伴慢性骨髓炎5例，足后跟足底软组织缺损伴跟骨表层组织缺损3例，跟骨骨髓炎伴窦道2例，皮瓣面积30 cm×10 cm～6 cm× 4.5cm，切取的腓肠肌瓣面积4 cm×3 cm～9 cm×6 cm，肌肉厚度1～3 cm，皮瓣面积比肌瓣的面积要大。

Methods Immunohistochemistry was used to evaluate AQP4 expression in testis and epididymis of rat.

采用免疫组织化学的方法，检测AQP4在睾丸和附睾的表达分布。

Preadipocytes from epiploon of newborn calf were cultured by monolayer adherent method, and total RNA of the preadipocytes was extracted on day 4,6,8,10,12,14 and 16 post-culture, respec-tively. The abundance of ADPN mRNA and HSL mRNA was detected by real-time fluorescent quanti-tative RT-PCR during differentiation period from preadipocyte to adipocyte.

选取犊牛大网膜脂肪组织，进行牛前脂肪细胞的单层贴壁培养，在细胞接种后第4、6、8、10、12、14、16d分别提取细胞总RNA，采用实时荧光定量RT-PCR方法，检测了体外培养的犊牛前脂肪细胞不同时期ADPN基因mRNA和HSL基因mRNA的表达情况。

The comparison between experimetal and calculated results indicated that the simulation model and equilibrium phase diagram calculation can exactly describe the elements distribution law and predict microstructure variation near interface.

计算结果表明：该模拟方法能够准确描述热等静压扩散焊接过程中的元素浓度分布规律，同时能够预测扩散反应层内的微观组织变化。

In order to study the mechanism by which estrogen effects on bone, the effect of estrone on expression of c-myc protein in the epiphyseal plate of fetal mouse long bone was detected by immunohistochemistry procedure in vitro.

为初步探讨雌激素对骨的作用机制，以体外培养的小鼠胚胎长骨作为模型，通过免疫组织化学方法检测雌酚酮对骺板静止区、增殖区和肥大区c-myc蛋白表达的影响。

Objective: To explore a feasible method and its significance of 3 - dimensional reconstruction in nasal cavity and ethmoid sinus.

三维重建技术是指通过图像处理和分析方法由二维生成和摄取人体三维组织结构，目前多用于CT 和MRI影像学。

The microstructure changes of pearlitic eutectoid steel induced by cold rolling were investigated by using SEM, TEM and XRD etc.

用SEM、TEM及XRD等方法研究了共析钢中珠光体经40％-90％的冷轧变形后所产生的显徽组织变化。

The clinical and pathological case information of CRC high-risk individuals were gathered and a population based case-control study was performed between and within three colonoscopic examinate groups of field screening, health examination and surveillance, and clinical symptomatic consultation. The distributions of age, gender and detective conditions of pre-cancerious alterations and CRC, as well as the relationship of pathological conditions and ages were analyzed between and within the three groups to evaluate the existent strategies of colonoscopy for CRC high-risk population.

2应用所建立的CRC高危人群临床病例信息科学采集与处理方法，对现场普查、体检复查和因症就诊三种不同来源的结肠镜受检人群进行成组资料的人群对照研究，分析比较三组受检人群年龄、性别构成特点以及对CRC癌前病变和CRC的检出情况、病理组织类型和分布特点，并对现有CRC及其高危人群结肠镜检查策略进行分析评价。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。