组织方法

Methods The levels of MMP2 and TIMP2 of 25 patients in decidua and villii from 25 patients with URSA were determined by immunohistochemical method.

以免疫组化染色方法检测原因不明复发性流产患者25例蜕膜和绒毛组织中MMP2、TIMP2的表达。

MethodsThe model of cerebral ischemia and anoxia in mice was established by decollation and ligating common bicarotid artery the effect of BVF on the survival time of the mice,and the time of catching their breath after mice decollated,and mouse brain capillary vessel permeability were observed.

方法采用小鼠断头、结扎双侧颈总动脉等脑缺血缺氧模型，观察苦菜总黄酮对脑缺血缺氧小鼠存活时间、断头小鼠喘息时间及脑毛细血管通透的影响；TBA法、NBT法测定小鼠脑缺血缺氧后脑组织MDA含量和SOD活性。

Histopathological changes of lung tissue were observed under light microscope. The protein expression of MMP (MMP-2, MMP-9) and TIMP (TIMP-1) in lung tissue were determined by immunocytochemistry. RESULTS: Decompensate respiratory acidosis (pH =7.20±0.04, PaCO2 =7.84±0.15) developed in group B.

用ELISA法测定大鼠血清MMP-2、MMP-9、TIMP-1活性及NE活性；弹力纤维染色标本进行弹性纤维相对含量的测定；免疫组化方法检测肺组织中MMP-2、MMP-9及TIMP-1的表达及光镜下进行病理学观察。

Background: Atrial septal defect is the most common congenital heart diseases .Exactly assessment of the site, shape, size and anatomic relation of the defect with its surrounding structures is essential for the clinical management of the patient.

研究背景：房间隔缺损(Atrial septal defect，ASD)的发病率在各种先天性心脏病(Congenital heart diseases ，CHD)中居首位，准确诊断缺损的部位、形态、大小及与周围组织的毗邻关系很大程度上决定了治疗方法的选择及治疗效果。

To build a Marxist learning party, it is necessary to overcome learning difficulties, enrich learning content, adopt a scientific learning method, establish an effective learning system, delayer the organizations, create a learning atmosphere, build an organization of learning party and cultivate leaders of a learning party.

建设马克思主义学习型政党，要努力克服学习智障，丰富拓展学习内容，坚持科学的学习方法，建立有效的学习制度，构建扁平化的组织结构，营造浓厚的学习氛围，建设学习型党组织，建设学习型领导干部队伍。

Objectives: To collect information of demology, sociological and epidemiology about female sexual workers in top grade entertainment place of Chengdu downtown district and to understand sexual health requirements of FSW related to their knowledge, attitudes and behavior, in order to carry out appropriate behavioral intervention.

目的：了解成都中心城区内高档次娱乐场所内商业性性服务组织形式及性交易特点，掌握该类场所女性性工作者特征，找到影响其认知、态度、行为的各种因素，了解其健康需求，探索接近该类型女性性工作者并对其进行性病/艾滋病健康教育和行为干预的可行方法。

The theoretical analysis and demonstrational analysis are combined in the thesis, and the market structure theory and the scale economy theory in industrial organization theory are applied in the study of the concentration ratio of highway transport market.

摘要本文主要采用理论分析与实证分析相结合的方法，将产业组织理论中的市场结构理论和规模经济理论运用到道路运输市场集中度的分析中。

Methods The MIN in 50 cases of laryngeal cancer were detected by the methods of PCR denature PAGE polymorphous markers BAT 26 and BAT 40. Results Twenty six percent of patients with laryngeal cancer had MIN.

采用 PCR-变性聚丙烯酰胺凝胶电泳等分子生物学方法用微卫星多态标记物 bat - 2 6和 bat - 4 0检测 5 0例喉鳞癌组织的 MIN。

Results After articular cartilage injury, the degeneration and denature of the cartilage occurred, and the cluster expressed collagen type Ⅹ and apoptosis appeared.Conclusion Apoptosis may play a key role in the pathogenesis and prognosis of osteoarthritis.

为进一步探讨骨关节病的发病机制，我们收集了骨关节病的临床标本，采用原位杂交及原位末端标记的方法，观察Ⅹ型胶原和凋亡细胞在骨关节病关节软骨组织中的分布，并探讨凋亡与骨关节病的关系。

Methods The cDNA retro-transcribed from equal quantity total RNA derived from rectal carcinoma or normal rectal mucosa were labeled with Cy5 or Cy3 fluorescence as probes respectively. The mixed probes were hybridized with the BioStar 4096 cDNA microarray.

分别抽提直肠腺癌和正常直肠粘膜的总RNA；通过逆转录方法将Cy3和Cy5两种荧光分别标记到两组cDNA上，制成探针；利用这种cDNA探针与含有4096条人类基因PCR产物的表达谱芯片进行杂交，扫描芯片荧光信号图像，计算机分析后比较两种组织中差异表达的基因。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。