组织方法

METHODSUsing the MTT assay, transmission electric microscope, DNALadder method, TUNEL method and immunocytochemistry, the inducing APO and antiproliferation of the extract of Rubia Cordifolia L for MGC803 cell line of gastric carcinoma were studied in morphology, biochemistry and immunocytochemistry.

方法］利用组织细胞培养技术及MTT法，通过电泳分析及多种细胞染色等方法，观察茜草提取物对胃癌MGC803细胞株的凋亡及抗增殖情况。

Methods Seral anti-P 53 antibody was detected with immuno-PCR.The expression of P 53 in tissue was detected with enzyme imunohisto-chemistry technique.

采用免疫PCR方法检测血清抗P 53 蛋白抗体，酶免疫组化方法检测组织P 53 蛋白表达。

Methods: thirty nine full term placentae of hbsag sero positive pregnant women were collected, in which 23 were detected as hbsag positive by sabc immunohistochemistry staining.

收集血清学hbsag阳性孕妇足月胎盘组织39例，经sabc免疫组化方法在其中23例中检测到hbsag，随机选取7例采用免疫荧光双标记方法结合激光共聚焦技术检测hbsag/annexin v。

At second, this paper base on marketing and the case of Sier Company and gives the analysis the project in detail by using a lot of market research and statistic information.

接下来从市场营销的角度出发，以思尔健康管理公司为案例，运用市场营销、管理学知识，通过大量的市场调研，运用了丰富、翔实的统计资料，通过PEST方法详细地分析了思尔公司的外部环境因素对公司营销策略的影响，并从组织结构、管理能力、人员状况等方面进行了深入地研究和探讨，并对其不足之处提出了改进的方法。

This experiment which adopting technology of examination of muscle, utilizing modern organized chemical method, using scanning photometer, doing a eight-week staminal training towards six ordinary examinee, analyses the samples of the vastus lateralis of the right leg before and after the experiment.

采用肌肉活检技术，运用现代组织化学方法，结合扫描显微分光光度计定量的方法，对6名普通受试者进行8周耐力跑训练。

Simultaneous determination of N, N-Dimethylaniline and phenol in wastewater by high-performance liquid chromatography with chemiluminescence detection; 2 rapid determination of rudimental trace aniline in indigotine by reverse phase HPLC; 3 determination of the levels of tetracycline and oxytetracycline residues in liver tissue by HPLC-CL; 4 rapid determination of Shikimic acid in taxodium distichum by ion-suppression reversed-phase HPLC.

第二节是高效液相色谱及其与化学发光柱后检测技术在药物分析中的应用，研究了四环素、土霉素的HPLC-CL方法，应用于动物肝组织中四环素、土霉素残留量的测定；并研究了用离子抑制-反相高效液相色谱法快速测定落羽杉中的莽草酸的新方法。

Methods Rats aging 4 weeks were immobilized by limb-tail fixation for 2 and 4 weeks respectively, and then the wet weight, gray weight, bone mineral density of femurs and tibiae were measured, the histopathological and histomorphological parameters also were detected, which were used to evaluate the effect of immobilization on bone content and bone structure of femurs and tibiae in growing rats. The VDR expression of femurs and tibiae in the growing rats was evaluated by immunohistochemistry and image analysis.

通过腿－尾固定法对4周龄幼鼠分别进行右后肢2周和4周制动，采用股骨湿重、灰重、骨密度测量，骨组织病理学和骨组织形态学参数测定等方法对比评价制动对幼鼠制动侧股骨和胫骨的骨量、骨结构的影响，采用免疫组织化学和图象分析方法检测制动对幼鼠骨VDR表达的影响。

The cleaner unhairing and liming technology was applied in pig garment leather and sheep garment leather. The physical and chemical properties of the resultant leather were tested, and the fiber separation was examined by SEM and Vis microscope analysis. All the results showed that this cleaner liming process is feasible.

论文最后将清洁化脱毛浸灰工艺在猪正面服装革和羊皮服装革的生产上作了应用，测定了成革的理化性能，并用组织学方法和扫描电镜观察方法检查了胶原纤维的松散情况，结果表明实行清洁化脱毛浸灰工艺是完全可行的。

The expressions of VEGF、bFGF、IL-1βin myocardium were measured by RT-PCR and ELISA. Other steps and methods were same with the second part. Results: 1. The cells in 2Gy, 4Gy groups and unirradiated group grew well. All cells in 8Gy and 12Gy groups were dead after irradiation.

30只大鼠随机分为注射上清液组和培养基组，除了采用RT-PCR和ELISE两种方法检测VEGF、bFGF、IL-1β在心肌组织内的表达外，其他实验步骤、方法及检测指标同实验第二部分。

Under the International Organization for Standardisation method, similar testing methods were adopted in Europe and many other countries.

在国际标准化组织的方法之下，类似检测方法已在欧洲和许多其它国家采用。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。