组织方法

Sixty-three cases of AFP-positive gastric carcinoma were divided into four subtype: hepatoid type 22 cases (34.9％), fetal gastrointestinal type 15 cases (23.8%), and mixed type of the hepatoid type with enteroblastic differentiation to be 24 cases(38.1%), and yolk sac tumor-like type 2 cases (3.2％).

应用光镜、电镜、组化和免疫组织化学方法，对87例AFP阳性胃癌中临床病理资料较完整的63例进行病理组织形态观察，并用2例3个月妊娠的胚胎胃肠组织以同样方法行相关的对比研究。

The third chapter aims to discuss the self-organization essence, self-organization growth mechanism, the pattern of self-organization evolving growth.The fourth chapter aims to discuss particularly the correlative mechanism between clusters dynamic core capacity and clusters sustainable growth, and the obtaining of cluster sustainable competitive advantage basede on dynamic core capacity and the fostering and newing of cluster dynamic core capacity based on discussing the rigid competitive advantage. The fifth chapter aims to explain the effecting mechanism of government influencing cluster self-organization-evolvement and the fostering and upgrading of cluster dynamic capacity based on the theory gist of government surpports .The sixth chapter aims to analyse the High-tech industrial clusters of Silicon Valley in American and Low piezoelectricity cluster of Liu shi in zhengjiang province and explains to how self-organization , dynamic core capacity and government surpports effect industrial clusters sustainable gowth.The dissertation's main innovation lies in the following :mechanism model of cluster sustainable growth is constructed under dynamic environment based on self-organization theory and dynamic core capacity theory .

本文共分6章，第1章主要阐述本论文的研究背景、研究问题和研究意义，综述相关的国内外文献，并提出本论文的研究逻辑思路、研究方法和主要内容；第2章在对产业集群本质再认识和集群持续成长内涵分析的基础上，从自组织理论和动态核心能力理论视角，构建了动态环境下集群持续成长机制的概念模型；第3章主要探讨了集群持续成长的自组织本质、自组织成长机理和自组织演化成长模式；第4章在分析集群竞争优势刚性的基础上，提出集群动态核心能力的概念，分析了集群动态核心能力的构成要素及其对集群持续成长的作用机制，探讨了集群动态核心能力的演进与产业集群持续成长的关联机理，并进一步探讨基于集群动态核心能力集群持续竞争优势的获取问题，最后是关于集群动态核心能力的培育与更新的探讨；第5章在论述政府对集群支持的理论依据的基础上，分别探讨了政府对集群自组织演进和对集群动态核心能力的培育和提升的作用机制；第6章实例研究，论文选取了美国硅谷高科技产业集群和浙江柳市低压电器产业集群进行实例分析，研究了自组织、动态核心能力和政府支持三大机制在集群持续成长过程中的作用。

Methods Malignant tumor tissues were obtained from 11 patients with colon cancer, 15 patients with cervical cancer, 10 patients with breast cancer, 9 patients with esophageal cancer, 12 patients with lung cancer and 16 patients with gastric carcinoma. Nonmalignant tissue was used as negative control. Three tumor cell lines were also studied. Hfgl 2 was examined both at the level of mRNA and protein by immunohistochemistry as well as RT-PCR.

采用免疫组化方法检测了11例结肠癌、15例宫颈癌、10例乳腺癌、9例食管癌、12例肺癌、16例胃癌患者的病理组织切片中hfgl 2及纤维蛋白的表达，分析二者的组织学关系，用RT-PCR方法检测了部分肿瘤组织与肿瘤细胞系中的hfgl 2 mRNA。

Methods: By reverse transcriptase polymerase chain reaction and immunohisto-chemical assay, the expression of Surviving gene and bcl-2 protein were detected in 16 cases normal tissue of bladder, 12 cases papillate tumor and 59 cases transitional cell cancer of bladder.

采用逆转录PCR方法检测了16例正常膀胱组织、12例膀胱移行细胞乳头状瘤和59例膀胱TCC组织Survivin基因的表达，同时用免疫组化学方法检测以上组织中bcl-2蛋白的表达，并将结果进行相关分析。

MethodsPrimary nasal pterygial tissues(15 eyes) and normal human conjunctival tissues(8 eyes) were determined by immunohistochemical staining, using monoclonal and polyconal antibodies specifically against MMP-3, LN and col Ⅲ. The intensity of reaction on individual sections was semiquantitatively graded.

方法应用S-P免疫组织化学染色方法检测15例翼状胬肉组织及8例正常球结膜组织中MMP-3、LN、col Ⅲ的免疫学定位及表达情况，用Masson及VG染色法检测各标本中胶原纤维及弹力纤维的分布情况。

Methods Sixty adult healthy male Wistar rats were enrolled in the experiment. PTSD-like rat model was established by single prolonged stress. There was no SPS stimulation in the control group and the rats of the other five groups were undisturbedly raised for 24 hours, 4 days,7 days,14 days and 28 days respectively after SPS stimulation.The expressions of glucocorticoid receptors in the locus ceruleus nucleus of all groups were detected with Nissl staining, immunohistochemistry,Western blotting and PCR, and image analysis and statistical analysis were performed.

成年健康雄性Wistar大鼠60只，使用无连续单一应激方法建立PTSD大鼠模型，分别取SPS处理后24h、4d、7d、14d和28d大鼠蓝斑组织，同时取正常蓝斑组织作为对照，应用尼氏染色观察蓝斑形态，采用免疫组织化学、免疫印迹和RT-PCR方法分别观察及检测各组蓝斑神经元GR表达的变化，并进行图像分析和统计学处理。

Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

Results 1.After treated by:program freezing,the maximum load of the allografts has no evident decrease and the cell activity of allografts was preserved well,the frozen hurt after program freezing was more slight than －80℃ deepfrozen,2.No evident reject reaction can be seen during the healing process after deep frozen and the reject reaction declined as the time pass by.3.Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

Material and Method: 40 paraffins of prostate carcinoma from 1996 to 2005 were collected.

材料和方法：搜集昆明医学院附属第一院和第三院1996年～2005年间临床资料完整的40例未经治疗前列腺癌患者的前列腺癌组织标本，应用免疫组织化学方法检测雄激素受体的表达。40例病例根据肿瘤的组织学形态进行病理分级。40例病例术前均行了盆腔前列腺CT扫描。

A multi-distance measurements method is employed in this paper. A single parameter fitting method and a double parameters (reduced scattering coefficient and amplitude factor) fitting method on the basis of the least square Newton fitting algorithm is used to derive the optical parameters respectively. For the finite-thickness homogenous tissue, it can be concluded that the rest terms in expressions of diffusion reflectance and transmittance for the corresponding tissue geometries that subtract the exponential term contained source-detector distance depend on the detected position weakly.

在多程测量的基础上，使用单参数最小二乘牛顿法拟合方法和双参数（约化散射系数、幅度因子）最小二乘牛顿法拟合方法，得到有限厚均匀组织的反射率分布和透射率分布公式中除去包含测量位置的指数项的其它部分对测量位置皆具有弱依赖性的结论，并对拟合结果进行了分析讨论；而对于有限厚平板状双层组织，可根据对反射测量结果及透射测量结果进行拟合所得的约化散射系数间的差异及常数因子的正负判断双层组织的可能结构分布。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。