组织方法

Methods Gastric cancer tissues, adjacent tissues and normal gastric mucosal tissues in 75 patients were studied, The expression of ubiquitin and 26S protesome activity were analyzed by Wes tern blot and fluorogenic technique, res pectively.

对75例胃癌患者分别在胃癌、癌旁及远端正常黏膜组织取样进行研究。采用免疫印迹方法分析泛素表达，免疫荧光方法分析265蛋白酶体活性。

The expression characterization of gibel carp Caghira gene in different tissues and stages of embryogenesis were analyzed by RT-PCR and whole mout in situ hybridization.

采用RT-PCR方法并结合整体原位杂交的方法分析了银鲫Caghira在不同组织中和胚胎发育不同阶段中的表达特征。

Methods The surviving was detected by immunohistochemical assay in urine sediments samples of the 75 cases of bladder tumor, 2 Glandulose cystitis, and 10 normal control.

方法用免疫组织化学方法检测膀胱肿瘤75例、腺性膀胱炎2例、正常对照10例的尿沉渣Survivin蛋白的表达。

Hemarthrosis and destruction of the disc played an important role in the pathogenesis of traumatic diseases in TMJs. Part 3 A comparative study of the histological changes in the TMJ after different treatment of unilateral fractures in the lambs The histological changes were recorded in the TMJ after different treatment of unilateral fractures.

实验三 创伤后应用不同方法治疗单侧髁状突骨折后TMJ组织病理学改变的比较研究本实验采用颏部撞击法造成幼年山羊的双侧TMJ损伤后，在左侧髁颈部切断髁状突，模拟单侧髁状突骨折，并应用不同的治疗方法进行处理，观察其关节结构的变化情况。

Methods Homozygous deletion of p16/MTS1 gene was examined by PCR methods in 68 cases of colorectal carcinoma.

方法采用聚合酶链反应方法，对68例结直肠癌组织中p16/MTS1基因纯合缺失进行检测。

Methods The au- thors selected 15 patients whose primary breast volume was less than 70ml.During augmentation mammaplasty on these patients,the inframammary creases were reconstructed by lifting and fixation un- der the pectorales major.

方法选原乳房体积单侧小于70ml 的15例求术者，在隆胸的同时，合理设计胸大肌下分离间隙，利用上提、固定乳房下分组织的方法重建乳房下皱壁。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

This classifier finished TM landcover by fuzzy logic reasoning, and the unclassified pixels increase K adaptively to be classified; It improved 2.7% and 2.9% in overall accuracy and Kapp coefficient compared with MLC, deceased 1% in Kapp coefficient and no change in overall accuracy compared with self-organizing network.

对拒分类的像元，自适应增加K值使其可分。该方法所得分类精度及Kapp系数与最大似然分类方法结果相比分别提高了2.7%和2.9%；与自组织网络相比，总精度相差不大，而Kapp系数低1%。

Serum estradiol level of rats was detected by means of radioimmunoassay. The positive staining and intensity of lipid in the meibomian gland alveolus was detected by Sudan Ⅲ staining.

实验评估：采用放射免疫分析方法检测血清雌二醇水平；采用组织化学苏丹Ⅲ染色方法对睑板腺腺泡上皮做脂质染色阳性和强度检测。

Methods In both malformed embryos induced by excess retinoid acid and normal KM mice,expressions of Dishevelled2 and Vangl2 in the epithelium of the palatal shelves and the mesenchyma were determined by immunohistochemistry and image analysis.

方法采用免疫组织化学及图像分析方法，检测维甲酸致畸小鼠和相应对照组小鼠腭板中Dishevelled2和Vangl2蛋白的表达状况。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。