组织方法

Methods ①In situ hybridization was applied to observe the changes ofhypothalamic CRHmRNA positive cells after PX and melatonin supplementation;②Immunohistochemistry was used to observe the changes of CRH positive cellsin hypothalamus and CRH staining in median eminence of neurohypophysis after PXand melatonin supplementation;③ELISA was applied to detect the changes of CRHconcentration in serum after PX and melatonin supplementation.

①运用CRHmRNA 原位杂交的方法来观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRHmRNA 阳性细胞的变化；②应用免疫组织化学染色的方法观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRH 阳性细胞和神经垂体正中隆起CRH 阳性染色的变化；③运用ELISA 法检测松果体摘除及补充褪黑素后血清CRH 浓度的变化。

Most of the experiment rats whose autonomic nerves controlling uterus were cut off couldn′t pregnancy,nidation was delayed in the other rats.

选用成年雌性 Wistar大鼠，采用外科手术方法分别在妊娠前和妊娠后切断支配大鼠子宫的植物性神经，观察了切断神经对 Wistar大鼠着床的影响，并用常规组织学方法观察了子宫肥大细胞数的变化。

Computer image analysis was used to measure the average optical density of synaptophysin.

方法采用免疫组织化学方法，观察突触素在不同周龄的人胎儿海马中的表达水平，利用计算机图像分析技术测量不同周龄阶段海马突触素表达的平均光密度。

Methods The biopsy specimens in paries anterior vaginase were obtained from 88 subjects undergoing hysterectomia,tension-free vagina tape,Burch procedures,who were assigned into control,pelvic organ prolapse(POP 28 cases) and SUI groups30 c...

方法采用免疫组化方法，测定30例压力性尿失禁患者、28例盆底器官膨出患者阴道前壁组织中TGF-β1及ER阳性率，并选择同期30例非卵巢功能性肿瘤和宫颈病变患者作为对照。

The method learns groovy method to undertake to two kinds of honeysuckle configuration histology contrast studies by crude drug, microscopical to the part the feature undertakes photomicrography.

方法按生药学常规方法对两种忍冬进行形态组织学对比探究，对部分显微特征进行显微摄影。

This paper reviewed the popular methods used in age determination,including the use of hard tissue and length frequency.

综述了目前应用较广泛的一些年龄鉴定方法，包括硬组织鉴定法和长度分析法两大部分方法，并分析了它们的优点和缺点。

But specifications written in formal are prolixity and boring, so it needs a structured way to decompose and organize for writing and understanding more easily.

但用形式化方法书写的系统规格说明往往是冗长而乏味的，为了帮助开发人员书写和理解形式化说明，需要一种结构化的方法来分解、组织形式化说明，使之具有灵活性和可管理性并具有良好的风格。

The reason of color deterioration, and the relation between tissue structure and rehydration properties were also analyzed.

总结了不同乾燥方法对产品颜色变化的影响结果及其原因，乾燥方法对产品复水性能的影响以及复水性能与产品组织结构内在的必然联系。

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

To search for the significance of immunohistochemical markers for the differential diagnosis of rhabdomyosarcoma and the clinical impact of different subtypes of rhabdomyosarcoma on prognosis.

目的 ：探讨横纹肌肉瘤的诊断和鉴别诊断中免疫组织化学标记的意义及不同类型横纹肌肉瘤对预后的影响。方法：应用病理形态学和免疫组化方法对 47例横纹肌肉瘤进行研究。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。