组织方法

The uncovered tracts and the liver wound surface were managed by some of the methods combined such as high frequency electrocoagulation, titanium clamp, suture, hemostatic gauze oppression, argon-beam coagulation, protein glue adherence, and Endo-GIA switcher. The total operative outcome, operating time, blood loss, postoperative complications and postoperative hospital stay were summarized in order to assess feasibility and safety of the clinical laparoscopic liver resection.Results: 1. Laparoscopic liver resection with clamp dissection method needs only the ordinary laparoscopic equipment. It has a low cost but causes a little more bleeding. 2. Laparoscopic liver resection operated by microwave tissue coagulation method had very little bleeding when dissecting hepatic parenchyma. The operative field is clear and the cost is low. It is a safe, convenient and effective method. 3. Ultrasound dissector is a relatively ideal appliance for laparoscopic liver resection at present because ofless bleeding during operation. But the speed is slow thus prolongs the operation time. Its separating intensity is not strong enough, and it can only be applied to patients without hepatic cirrhosis. 4. Ligsure has clamp dissection and electrocoagulation functions. It can carbonize hepatic tissue including the tracts whose diameters are less than 7mm. It has some advantage in dissecting. However, it has the shortcomings of the clamp dissector during coagulating. 5. Hand-assisted laparoscopic liver resection can make use of the flexibility and feeling of the operators left hand.

结果：1、钳夹分离切肝法不需特殊仪器设备，只要具备常规的腹腔镜器械即可实施，成本低，肝断面较易渗血；2、微波固化切肝法肝实质出血很少，视野清晰，成本低，操作简单，是一种安全、简便、有效的方法；3、超声刀是目前较为理想的切肝器械，术中出血少，但是，切割速度慢，手术时间长，而且，切割强度有限，适用于无肝硬化的肝切除；4、Ligsure具有挤压粉碎及高频电凝止血两大功能，可使包括7~以下管道的肝组织碳化、形成焦痴，具有一定的优势，但是，也存在分离钳电凝止血的缺点；5、手辅助腹腔镜肝切除可以利用术者左手的灵活性及手感，便于术中显露、分离、控制出血等，可随意协助右手及助手的主要操作，缩短了手术时间，大大提高了腹腔镜肝切除的安全性，李朝龙等的改良方法，同样达到了手辅助目的，又节省了费用；6、小切口腹腔镜辅助切肝法可以使用剖腹肝切除常规器械及剖腹肝切除技术，操作简单、可靠，适用于肝左外叶切除及右肝第V段切除；7、Endo一GIA切肝法在离断肝组织的同时闭合管道结构，多用于肝左静脉、门静脉分支、管径较大胆管的切割，缺点是不能用于较厚肝组织的切割，而且价格昂贵；8、临床巧例腹腔镜肝切除均获得成功，手术时间最短1.5h，最长sh，平均125 min，多数在100 min左右，手术出血量最少50 ml，最多500 ml，平均1 78 ml，除2例合并严重肝硬化的原发性肝癌患者术后出现少量腹水，1例术后发生胆漏外，其余无并发症，发生胆漏的1例患者术后住院40d，多数在术后一周左右出院，术后住院时间5一40d，平均gd。

Histological structure of hair root and limed skin was separately observed by optical microscope,scanning electron microscope and by tissue section method during different phases of enzymatic dehairing,which could be used to interpret mechanism of action by different depilatory techniques.

用光学显微镜、扫描电镜及组织切片法分别对酶脱毛不同时间段内的毛根组织结构及不同脱毛方法的灰皮进行组织结构观察，结果可以解释说明不同脱毛方法的作用机理。

Perspective of the thesis is from the social development and enterprise progress, to enhance competitive advantage of technological enterprises as the goal, describe the necessary to resolve the issue of the organizational structure by a comparative analysis, as well as in practice, optimization of the organizational structure has practical significance, the thesis is on the basis of optimization analysis of organizational structure of Shenzhen MG technology company, and uses the four-cycle perspective to describe the cyclical dynamic reconfiguration of the organization optimization problem, through the planning, startup, diagnosis, design, reconstruction, monitoring , as well as steps to improve the optimization of organizational structure, provide a new way of thinking and problem-solving approach to improve the efficiency of organization for similarity technology enterprises.

本文从社会发展、企业进步的视角出发，以提升科技企业竞争优势为目标，阐述了解决企业组织结构问题在理论上的必要性，以及组织结构优化在实践中所具有的现实意义。本文在对深圳MG科技公司组织结构现状进行分析的基础上，采用四循环法这种以周期性的观点描述企业动态组织优化问题的方法，通过策划、启动、诊断、设计、重构、监控、以及改进等步骤逐步实现对深圳MG科技公司的组织结构优化，所得的研究成果为类似科技企业进行组织结构优化提供了新的解决思路和方案。

To investigate the method of RNA isolation from human embryonic tissues and the factors influencing the quality of RNA, the RNA from human embryonic tissues obtained with drug-induced labor or non-drug induced labor were isolated by using grind with liquid nitrogen or homogenizer without liquid nitrogen.

为探讨人类胚胎组织完整RNA的分离方法及其影响因素，采用液氮研磨及匀浆器两种制备组织匀浆方法，对不同引产方式的人类胚胎组织进行总RNA分离，并对RNA质量进行鉴定。

Objective To study the significance of expression of cyclooxygenase-2(COX-2)and vascular endothelial growth factorin breast cancerand its correlation.Methods Paraffin imbedding tissues from 60 cases with BC were detected for expression of COX-2 and VEGF by immunohistochemistry,the relationship of its expression with clinical pathological parameters were analyzed.

目的 探讨环氧化酶-2（COX-2）和血管内皮细胞生长因子在乳腺癌组织中的表达及其表达之间的关系方法 S-P免疫组织化学方法检测60例乳腺癌石蜡包埋组织中COX-2和VEGF的表达，分析其表达之间的关系及其与临床病理参的关系。

The wound surfaces in the experimental group were treated by arnebia root oil and those in control group were treated by petrolatum gauze.

用组织学、组织化学、电镜等方法观察创面的组织结构和愈合率、用Western-blot，RT-PCR方法观察2组的bFGF及其mRNA表达。

In view of the management strategy of Publishing House A and its present human resource management situation, which is characterized by human resource department still functioning mostly in just giving advice, organization structure unadaptable to the companys development strategy, lack of systematic personnel training, short of viable performance appraisal system, unsound motivation of compensation system, the author, by using the theories and methods of the subjects such as human resource management, organization behavior, accounting, strategy management, investigation and data analysis and statistics, brings forward 6 human resource strategies: to establish human resources management type playing strategic guidance, to change the organization structure from a decentralized line-of-business organization structure to amatrix one, to establish a learning organization, a scientific performance appraisal system, an all-round motivation mechanism and a human resources precaution and alarm system.

本文根据A出版社的经营战略，针对A出版社人力资源部门的作用仍然停留在职能、参谋部门；组织结构不能适应组织的总体战略要求；培训体系尚不完善；缺乏一套切实可行的绩效考核体系；薪酬体系的激励作用不明显的实际情况，运用人力资源管理、组织行为学、会计学、战略管理学、调查与数据分析、统计学等其他边沿学科理论和方法，笔者提出了六条人力资源战略：建立战略导向型人力资源管理；直线职能制组织结构向矩阵式组织结构的转变；建立学习型组织；建立科学的绩效考核体系；建立全方位的激励机制；建立人力资源预警系统。

Methods: cell biological assay, histochemical staining of senescence associated-β-galactosidase and immunohistochemical staining of proliferating cell nuclear antigen wereused. Results: chondrocytes exhibited various types of amitotic figures.

应用细胞生物学方法、衰老相关-β-半乳糖苷酶组织化学染色和增殖细胞核抗原免疫组织化学方法，研究正常成人和正常成体大鼠气管软骨组织动力学。

Exfoliated cells from nasoparynx of patients with definite or suspected nasopharyngeal carcinoma were examined for EBNA by anticomplement immunoenzymatic method.

本文用抗补体免疫酶法检查鼻咽部脱落细胞的EBNA，结果①79例鼻咽癌病人全部阳性，其阳性检出率比细胞学(87.3％)和组织学(91.1％)方法高；②4例初次检查EBNA阳性，但细胞学和组织学阴性者，3个月内复查也找到癌细胞；③18例头颈部其他肿瘤病人和21例胎儿全部阴性，并为细胞学和组织学方法所证实。

Objective To study the relationship between IL-1,MMP-13and pathogenesis of knee osˉteoarthrosis.Methods IL-1and MMP-13were measured concurrently in synovial fluid samples from23patients with mild OA,28patients with severe OA and6patients with disciform meniscus.The determinations of positive rate of cells in genual synovium with IL-1and MMP-13were carried out using immunohistochemical techonology.

目的 探讨白细胞介素-1（IL-1）、基质金属蛋白酶-13（MMP-13）在膝关节骨性关节炎发病中的作用方法提取23例OA病变轻微患者、28例OA病变严重患者、6例对照者的滑膜组织及关节液，采用免疫组织化学方法检测膝关节滑膜组织中IL-1、MMP-13的阳性细胞率及通过夹心ELISA法测定膝关节滑液中IL-1、MMP-13含量。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。