组织方法

The coil spring were kept constant and recorded for 0hr, 1 hr, 2 hrs, 4 hrs, 8 hrs, 12hrs, ldays, 3days or 7days. Upon completion of experiments, the maxillae were removed. The specimens were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer for 24 hrs and decalcified in 10% ethylene diamine tetraacetic acid at room temperature for 5 wks.

本研究通过建立正畸大鼠牙齿移动动物模型和体外细胞培养，结合细胞力学试验方法和分子生物学测试技术，拟从组织、细胞水平以及分子生物学角度探讨ATF4在正畸牙齿移动过程中对牙周组织改建的影响及作用机制，进一步为正畸牙齿移动提供理论和实践依据。

Enzyme immunoassay was used to detect Apelin in plasma and myocardia. RT-PCR was used to analyze the relative amount of mRNA of Apelin and APJ in the myocardia.

酶联免疫法测定血浆和心肌中Apelin含量，RT-PCR法测定心肌组织Apelin、APJ mRNA水平，Western blot方法测定心肌组织中APJ蛋白水平。

The influence of overlaying tissues on the measurement of the buried tissue was analyzed by experimental method.

实验研究了外层覆盖组织对埋藏组织监测的影响，并验证了利用归一化检测灵敏度修正这种影响的方法。

Our results show that the frequent finding of HPV 6/11 DNA in oral SCP had close association between oral papillomas and HPV 6/11 infection.

结论原位杂交方法可以检测口腔SCP组织中HPV DNA的存在并能准确组织定位，进一步支持HPV 6/11感染与口腔SCP的发生密切相关。

Ultrasonic tissue characterazation can assess pathological changes of myocardial tissue based on detecting and quantitating the acoustic properties of myocardium .

超声心肌组织定征技术(ultrasonic tissue characterization，UTC)是通过对心肌物理状态的变化即声学特征改变的检出和量化来判断心肌细微组织病理变化的方法。

Through investigating and studying some representative enterprise.the result shows that the current situation of software development in our country is not optimistic.Some software development organization can produce a few successful software.the reason owes to the endeavor of some outstanding individual and group but not to reduplicating using successful software process.

通过对国内一些中小软件企业的调研，结果显示我国的软件开发现状十分不容乐观，一些软件开发组织之所以能生产出个别优秀的软件产品，往往归功于软件开发组的一些杰出个人或小组的努力，而不是通过成功的软件过程的重复使用，开发组织大都没有总结出经过自身验证的开发方法和软件过程。

In this thesis, the phase diagram of Zn-Al binary system on 10~69.5 w% was reinvestigated by using differential scanning calorimeter, X-ray diffraction and pushrod dilatometer. The process of isothermal transformation during the aging of high-Aluminum-Zinc-based alloy was studied by using differential scanning calorimetry, and the aging behavior of the alloy was analyzed at the aspect of thermal analysis kinetics. The change of microstructure which the alloy was being heated was observed on high-temperature metallurgical microscope. The microstructure and wear-friction properties were compared among the alloys treated with several different heat treatment processes, and a model for heat treatment processes was proposed based on artificial neural network,so as to predict the relationship between friction coefficient and parameters of aging treatment such as aging temperature and time.

本文采用高温X射线衍射、示差扫描量热分析、热膨胀等方法重新测定含锌量范围为10％～69.5％的Zn-Al系二元相图，利用示差扫描量热分析法，研究高铝锌基合金时效等温转变过程，并从热分析动力学的角度分析高铝锌基合金合金时效行为；使用高温金相显微镜观察合金加热时的组织变化；比较几种不同热处理工艺对高铝锌基合金显微组织与耐磨性能的影响并利用人工神经网络建立高铝锌基合金热处理工艺模型，预测合金摩擦系数随时效温度及时间变化规律。

Is there any way for local human rights organizations - real, operational, organizations that are not globally oriented - to substantiate their particular focus without reneging on universal demands?

地方人权组织--实在的、能有效运作的、非全球性的组织--有何方法在不违背普遍的要求的前提下来实现他们特殊的关注？

The tumor-bearing mice received corresponding treatments for 15 consecutive days, after which their thymuses and spleens were isolated and weighed to calculate the thymus and spleen indices. The tumor mass was also weighed and the tumor inhibition rate calculated.

连续用药15d。第15天处死小鼠，计算胸腺指数、脾指数；剥离瘤块称重并计算抑瘤率；用免疫组织化学方法检测各组荷瘤小鼠肿瘤组织的增殖细胞核抗原的表达强度。

Methods: Immunohistochemicl method was used to detect the expression of Ki -67 in human villi and GTD.

采用免疫组织化学的方法，观察正常早期妊娠绒毛和妊娠滋养细胞疾病组织中Ki-67的表达。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。