组织方法

Model of animals were established by putting rats in a container with slippy inner wall and half-full of water in it, letting them swimming freely till died of being drowned. Each rat from different groups was killed by breaking its neck except drowning group, then BW and SO_2 was detected, and its chest was cut open rapidly, lung was taken out and weight was measured accurately to count lung coefficient. Its lung appearance was observed and lung tissue was fixed with formalin, which was made into pathology slices with HE staining, and pathology change of the lung tissue was observed;Expression of IL-8 and IL-10 in lung tissue was detected with Immunocytochemistry, and correlation between them was analyzed .

动物模型制作采用将大鼠放入盛有黄河水的内壁光滑的容器内，让其自由泳动自然溺死，除溺水致死组外，各组动物分别在规定的时间点断头处死，行体重和血氧饱和度等指标检测，并在冰盘中迅速开胸取肺，准确称重以计算肺系数，观察各组大鼠肺脏外观，取肺组织福尔马林固定，制成病理切片HE染色，光镜下观察肺组织病理改变；用免疫组化的方法检测肺内IL-8、IL-10的表达，并对其相关性进行分析研究。

Current, the various organization of the party had had soberer and soberer knowledge to this one job, the organization that taking cogenter and cogenter and effective new method, new move to strengthen and improve party of the basic level in enterprise of blame state ownership is built.

目前 ，党的各级组织对这一工作已经有了越来越清醒的认识，正在采取越来越切实有效的新方法、新举措来加强和改进非公有制企业中基层党的组织建设。

Commassie brilliant blue staining can clearly display the different structures of Sphaerotheca fuliginea, such as germinating conidia, initial germtubes, hyphae and conidiophores, and hardly stain the host tissue, presenting a striking contrast between fungus and host tissue.

考马斯亮蓝组织透明染色方法可以清楚观察到白粉病菌的5个发育阶段，即萌发的分生孢子、初生芽管、胞间菌丝、分生孢子梗和后期菌落。考马斯亮蓝几乎使寄主组织不着色，不产生背景色干扰，而菌体变深蓝色。

Up to now, ultrasound indentation technique has been widely used for assessing for the stiffness of tissue including residual limbs, diabetic feet, fibrotic neck induced by radiotherapy, spinae , and articular cartilage, which indicated that the technique can provide a quantitative measurement of tissue stiffness.

应用超声印压(Ultrasound indention，US indention)方法，已成功检测了糖尿病人足底、残肢、放射治疗前后颈部、脊柱、关节软骨等组织的硬度变化，表明用该法可定量检测因组织病变而引起力学特性改变。

But programs set, time of course, amount of curriculum, teaching condition, and teaching form are still insufficient.4The reasons why other universities and colleges do not set elective physical education course are mainly due to leaders" consciousness, quantity and quality of physical education teachers, the field and equipment.5The reform and development of elective physical education course in universities and colleges should incarnate the guideline "health first. people oriented". Teaching content should be pertinent, scientific, arranged, and systematic, so as to build up a teaching content system combined theory, skills and abilities. Set programs on base of students" choice, at the same time, give attention to the requirement of social development and students" interest, and make differential treatment according to gender. The classroom teaching should be sportful, joyful, and successful. The organizing of course could be mutual choice, compensating service, both in and out classroom. The score could be graded synthetically assess knowledge, innovation, and effect.6Strengthening the management to elective physical education course, improving teachers" comprehensive quality, making full use of fields and stadiums are all supporting conditions for the development of elective physical education course.

但是开设的项目、开课的时间、教学时数、教学条件、教学组织形式还难以满足学生学习和掌握知识和技能的需要。4、未开设公共体育选修课的高校其主要原因与领导的认识程度、体育教师的数量和质量、体育场地器材等有关。5、高校公共体育选修课的改革发展应体现&健康第一、以人为本&的指导思想：教学内容突出针对性、科学性、层次性、系统性，构建理论、运动技能、能力培养相结合的内容结构体系；运动项目设置以大学生对体育的选择性为基本前提，同时兼顾社会发展的需要和学生兴趣的需要，注重性别差异与因材施教；教学方法力求教懂、教会，体现课堂教学的娱乐性、快乐感和成功的喜悦：教学组织实施双项选择、有偿服务、课内课外相结合等多种形式；学习成绩实施知识+创新+效果的综合考核评价方法。6、加强公共体育选修课动态管理，提高教师综合素质，挖掘现有体育场馆设施的潜力，充分提高利用率是公共体育选修课改革发展的基本保障条件。

objectiveto evaluate and explore the reconstruction technique of straddling region reversal flow axial composite auricular island flap with blood-supply from supraorbital artery or supratrochlear artery for repairing large alar defects.methodsfrom may of 2004 to macth of 2008, 6 patients with the lager alar defects were studied.

目的：探讨以眶上动脉或滑车上动脉为供血源的跨区反流轴型耳廓复合组织岛状瓣，修复大面积鼻翼缺损的临床应用特点。方法：从2004年5月～2008年3月间，我科选用游离眶上动脉或滑车上动脉与颞浅动脉额支的吻合支为供血源所营养耳廓复合组织瓣，经皮下隧道转移至鼻翼缺损区，ⅰ期修复6例大面积的鼻翼缺损的患者。

By using integration classification systems, subject thesauri and semantic metadata, we can realize the knowledge organization of digital library.

利用集成分类法、主题词表和语义元数据构造数字图书馆知识组织系统的方法，可以实现数字图书馆的知识组织。

To investigate the expression of T lymphoma invasion and metastasis inducing factor 1 (Tiam 1) in tissues of thyroid carcinoma, and to analyze the relation between its expression and invasion and metastasis of thyroid carcinoma.

王化修，甲状腺癌中Tiam 1的表达及临床意义目的探讨T淋巴瘤侵袭转移诱导因子1的表达与甲状腺癌侵袭转移的关系。方法采用免疫组织化学S-P法检测Tiam1在89例甲状腺癌及其淋巴结转移癌的石蜡组织标本中的表达。

Objective To observe expression of T lymphoma invasion and metastasis inducing factor 1 (Tiam 1) and proliferating cell nuclear antigen in gastric cancer and investigate their relationship with clinicopathological parameters.

目的探讨T淋巴瘤侵袭转移诱导因子1(Tiam1)和增殖细胞核抗原在胃癌中的表达及其与临床病理参数的关系。方法采用免疫组织化学法对60例胃癌组织及其相应正常胃黏膜组织标本进行检测。

Design one primer pairs for EphB2 receptor and one primer pairs for GAPD as internal control. The RT-PCR was used to analyze the expression of EphB2 receptor tyrosine kinase in gastric cancer, colon cancer, lung cancer, liver cancer, kidney cancer, spleen cancer and abdominal wall cancer. The results were analyzed using Bio-RAD UV transilluminator with Quantity One software system.

采用RT-PCR方法，分别检测胃癌、结肠癌、肺癌、肝癌、肾癌、脾癌、腹壁癌7种肿瘤组织及相应邻近正常组织中EphB2受体的表达，应用Bio-RAD凝胶成像系统及在Quantity One软件辅助下分析测定表达结果。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。