组织方法

Objective To investigate the expression and significance of Ki67,cytotoxic proteinsTIAI,GranzymeB in nasal lymphoma.Methods Paraffin-embedded tissues of34cases of nasal NK/T-cell lymˉphoma,8midline B-cell lymphomawere studied using immunohistochemical method.

目的 探讨核抗原Ki67和细胞毒蛋白TIAI、GranzymeB在鼻腔淋巴瘤的表达及意义方法以NK/T淋巴瘤34例石蜡组织标本研究对象，同部位B细胞淋巴瘤8例为对照，行免疫组织化学染色。

Leave;phenol content changes in different parts of Pistacia vera L. and P.chinensis Bge in the growth process;phenol extracte, identificate;browning change in cell ultrastructure of the tissue.

其内容包括阿月浑子总酚提取方法，不同组织中的总酚在生长过程中的动态变化；酚类物质的提取、鉴定；褐变发生时组织细胞超微结构的变化。

METHODS A semi-quantitative reverse transcription polymerase chain reaction were performed to evaluate the expression of myostatin in the skeletal muscle of 5 Polymyositis patients and 4 healthy controls.

采用半定量RT-PCR方法检测5例多发性肌炎肌肉组织和配对非肌肉病对照者肌肉组织Myostatin mRNA表达及其与临床病理特征的关系。

The distribution of γ-aminobutyric acid and serotonin (5-HT) immunoreactive neurons in the protocerebrum and deutocerebrum of the butterfly Minathyma schrenckii were studied with the Colophony-paraffin embedding serial section technique and the Streptavidin-peroxidase immunohistochemical method.

采用树脂石蜡(Colophony-Paraffin，CP)组织包埋切片技术和链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-Peroxidase，SP)免疫组织化学方法，首次研究了 GABA 和5-HT 免疫阳性神经元在白斑迷蛱蝶前脑和中脑中的分布。

To observe the change of the SP- and CGRP- immunoreactive fibres in the pulpal tissue of rat by immunohistochemical method.

利用免疫组织化学方法观察大鼠牙齿开髓后2h、4h和急性牙髓炎2h、4h组牙髓组织中SP、CGRP免疫阳性纤维的变化。

Methods: The fibroblasts companying human hepatoma were primarily cultured with explant culture method, first passaged after they spread to the bottom of the culture bottle, and pured by enzyme digestion and repeated strapping the cells were identificated through observing the morphologic change using invert microscope and detecting expression of vimentin, keratin by iMmunohistochemical method.

应用组织块培养法进行人肝癌伴生成纤维细胞的原代培养，细胞铺满培养瓶底后首次传代，通过胰酶消化法和反复贴壁法进行成纤维细胞的纯化；通过倒置显微镜观察细胞形态、免疫组织化学方法检测细胞膜波形蛋白、角蛋白表达情况，对细胞进行鉴定。

Methods We reported a case of PXA located in the cerebellum of a young adult in view of its extremely unusual location. Morphologic observation, immunohistochemical staining using DAKO EnVision system , histochemical stains including hematoxylin eosin and Gordon Sweet's reticulin methods were used.

采用免疫组织化学DAKOEnVison法、组织化学网质纤维染色和HE染色及形态学观察的方法，报道 1例极罕见于年轻人小脑的多形性黄色星形细胞瘤，探讨其临床病理特点。

Objetive:To investigate the histopathological features and detect the mutation of JAK2V617F in PV、ET and IMF;to analyze the effects of the stages of myelofibrosis and the mutation on the number of leucocyte in PV and ET patients;Methods: the morphological features of 69 patients who dignosed in the first time were studied by HGF and Gomori stains ; the mutation of JAK2V617F was detected in 38 blood or marrow samples of PV、ET and IMF patients by real-time PCR;the effects of the stages of myelofibrosis and the mutation on leucocytes were analyzed;Results: Different stages of reticulin were observed in 15 PV and 15 ET patiens'marrow sections,and the cytosis、the pleomorphism and the bone trabecula side location of megakaryocytes were observed in all PV、ET and IMF patients;the means of leucocyte in patients with and without JAK2V617F were 20.4×109/Land 11.4×109/L respectively;the P value was 0.367 as comparing the leucocytes among different stages of reticulin increasing.

目的：探讨PV、ET、IMF患者的骨髓病理特点并且检测JAK2V617F突变的发生率，分析骨髓中纤维增生程度以及JAK2V617F对PV、ET患者白细胞数的影响；方法：69例初诊患者的骨髓切片经HGF染色以及Gomori染色后进行形态学研究；并利用real-time PCR法测38例患者的骨髓或者外周血的JAK2V617F突变；分析骨髓切片中纤维组织增生程度以及JAK2突变对患者白细胞数的影响；结果：15例PV和15例ET患者的骨髓切片中可以观察到不同程度的纤维组织增生，所有PV、ET、IMF患者骨髓病理中可见到巨核细胞增多、巨核细胞多形性改变以及巨核细胞定位于骨小梁旁；12例PV、4例ET、4例IMF中检测到JAK2V617F；有突变和无突变患者发病时白细胞数均值分别为20.4×109/L和11.4×109/L，比较不同纤维增生程度的PV、ET患者的白细胞数均值，P值为0.367。

Methods:Under the guidance of computer stereotactic equipment, the biopsy needle was introduced into the lesion in question which was impalpable but recognizable roentgenologically .

它是在常规乳腺X线片观察分析的基础上，对临床触不到，而X线片显示的乳腺小结节，微小钙化，局限致密浸润，通过电子计算机立体定位仪指导，将乳腺穿刺针直接刺入可疑病变区，取得活体组织标本，进行组织病理学检查的方法。

The adipose derived stem cells of inbreds train of rat wereinduced to differentiate into schwann-like cells and the cells were injectedinto the acellular nerve allograff.The proliferation or adhersion of these cells on the graft were observed by inverted microscope or ScanningElectronic Microscope. Activity of cells were detected using MTT. 10mmnerve gap of inbreds train of rats in two group were bridged by tissue-engineered peripheral nerve or autogenous nerve, the effect wereappraised by naked eye, recovery rate of sciatic function index,nerve-electrophysiological, histology, Transmission ElectronMicroscopy and quantitative analysis of recovery rate of myelinated fiberpopulations,diameter of myelinated fiber and thickness of myelin sheat.

按照前述方法培养并诱导近交系大鼠脂肪干细胞向类Sehwann分化，将诱导分化的脂肪干细胞悬液注入已制备的去细胞同种异体神经支架管中，倒置显微镜观察细胞生长情况；扫描电镜下观察细胞在材料上附着情况；四唑盐比色试验测定细胞活性；取两组近交系大鼠，分别用组织工程化神经和自体神经桥接10mm神经缺损，通过大体观察、坐骨神经功能指数恢复率的测定、神经电生理的测定、组织学光镜观察、透射电镜观察、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定等指标评价实验效果。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。