组织方法

The dynamic changes of the number of T or B lymphocytes and the content of immunoglobulin G, IgM, IgA in peripheral blood, the number of CD〓, CD〓 lymphocyte subpopulation, T〓 cells and IgG, IgM, IgA antibody-producing cells, the ratios of CD〓 and CD〓 T cells in immune organs (thymus, bursa of Fabricius and spleen) and immune tissues (cecal tonsils and Harderian glands), the content of IgA, IgM, IgG in local fluid (tear, tracheal fluid, intestinal fluid and bile) of one-day chicks infected with chicken infectious anemia virus and CIAV-infected chicks immunized with Newcastle disease vaccine were determined respectively by immunohistochemistry, immunogold-silves and immunoenzyme staining in order to reveal mechanism of immunopathogenesis of chicks infected with CIAV and influence of immune responses of CIAV-infected chicks to ND vaccine.

中文题名雏鸡感染鸡传染性贫血病毒后免疫变化和对新城疫疫苗的应答及其免疫机理副题名外文题名 Immune changes of chicks infected with chicken infectious anemia virus and immune response and mechanism to Newcastle disease vaccine immunity 论文作者郑世民导师刘忠贵教授学科专业基础兽医学研究领域\研究方向学位级别博士学位授予单位东北农业大学学位授予日期2002 论文页码总数105页关键词鸡传染性贫血病雏鸡新城疫疫苗馆藏号BSLW /2003 /S858 /9 本项研究采用免疫组织化学、免疫胶体金及免疫酶方法，首次对CIAV感染1日龄雏鸡和CIAV感染雏鸡ND疫苗免疫及vNDV攻击后，其外周血液的T细胞、B细胞数量和IgG、IgM、IgA含量；中枢免疫器官胸腺和法氏囊，外周免疫器官脾脏及呼吸道和消化道局部粘膜免疫组织哈德尔腺和盲肠扁桃体的ANAE〓、CD4〓、CD〓T细胞、IgG、IgM、IgA抗体生成细胞数量和CD〓与CD〓T细胞比值以及泪液、气管液、肠液、胆汁的IgA、IgM、IgG含量的动态变化规律和CIAV感染对ND疫苗的免疫保护率等进行了全面系统地研究。

Purpose Histological, histomorphological analysis and biomechanical test methods were used to analyze the bony ingrowth and shear strength conditions in the interface of bone and implants of 4 groups.

目的 通过对骨-植入体界面进行组织学和组织形态学的比较分析和生物力学测试，分析BMP及各种非骨水泥固定方法在促进骨长入骨-和植入体界面、增强固定强度方面的作用。

Methods:Computer-assisted quantitative image analysis was used to determine the histomorphology of 7 specimens of NP and 30 specimens of BPH with HE staining.

应用HE染色和计算机辅助图像分析的方法，对 7例正常前列腺和 30例前列腺增生患者前列腺组织进行组织形态学定量分析。

Objective To evaluate the role of histopathologic examine in cutaneous vasculitis.

目的：评价组织病理学在血管炎诊断中的价值。方法：本文对180例经组织病理诊断为皮肤血管炎的病例，进行系统的对照分析。

Objective To establish histopathological methods which can identify opportunistic fungi in tissues.

目的探索在组织中鉴定重要条件致病真菌的组织病理学方法。

The expression of Bcl-2 and Bax and Bak protein were examined with immunohistochemical method in the experimentation, the relationship between the protein expression and the clinical features of laryngeal carcinoma, as well as the relationship among them were discussed. We could probe into the safe incisal range of removing laryngeal cancer determined from the albumen balance of cells to conduct our clinical work.

本实验旨在通过免疫组织化学方法，检测Bcl-2、Bax和Bak蛋白在喉鳞状细胞癌组织中的表达情况，探讨它们与喉癌的临床指标的关系，探讨三者之间的关系，并从蛋白质水平探讨喉癌手术的安全切缘的范围为临床治疗提供参考。

Under the frame of inframarginal analysis, this thesis applies the transaction cost theory and the social capital theory to analyze peasants' choice of the different organizations and the various levels of the organization, also it combines questionnaire investigation and case interview to make an empirical research.

论文在超边际分析的理论框架下，运用新制度经济学的交易费用理论和社会学的社会资本理论，对农民配置人力资本的组织形式选择及农民普遍选定的经济组织内部的层次差异进行理论分析，并采用问卷调查与个案访谈相结合的方法对其进行了实证研究。

It also puts forward some methods, such as the introduction of negative entropy flows, improvement of organization mechanism and application of the passive factors, to strengthen the activeness, creativeness and inheritage of information consultation so as to insure the steady development of information consultation under the condition of unbalance.

同时，根据耗散结构理论的指导，提出通过进一步引入负熵流、改进组织机制、激活系统内部积极因素等方法，不断增强信息咨询的主动性、创造性和继承性等，以加强信息咨询系统开放、创新、协同和自组织等功能，保证信息咨询在非平衡态能够稳步发展。

With the development of new high-flux experiment technology—proteomics, we could wholy、 dynamically and interrelatedly investigate the differentially expressed proteins among chronic sinusitis, nasal polyps and normal nasal mucosa tissue.

随着蛋白质组学这一新兴科研方法的出现，使我们能在同一实验体系中高通量筛选慢性鼻窦炎、鼻息肉、鼻息肉病和正常鼻粘膜组织间差异表达的蛋白质，从而探索不同病变组织之间生命活动的差别。

Methods Five groups (n=24) of SD rats were randomly assigned to received intrapleural injection of dexamethasone, dipopolysaccharide, erythromycin, hypertonic saline and normal saline, respectively. The AQP1 protein in pleural was detected with immunohistochemistry. The mRNA expression of AQP1 under stimulations at different time points was measured by real time RT-PCR.

SD大鼠按实验设计分为5组(n=24)：地塞米松组、内毒素组、红霉素组、高渗盐水组及对照组，通过免疫组织化学染色测定AQP1在胸膜上的表达和定位；采用Real-time RT-PCR方法对不同刺激因子作用下不同时间胸膜组织AQP1的基因表达进行定量分析。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。