组织方法

objective:our aim is to investigate the relationship of vegf c,vegf d and lymph node metastasis in pancreatic cancer,and to elucidate the role and significance of the cancerous peripheral lymphatic tissue.methods: the expression of vegf c and vegf d was assayed by means of immunohistochemistry in 30 pancreatic carcinomas.results:the positive rates of vegf c、vegf d were 73%(22/30)、57%(17/30) respectively in pancreatic cancer.the expression of vegf c、 vegf d in cancerous invasive edge was significantly higher than that in the center of cancerous tissues.there was no correlation between the expression of vegf c、vegf dand the site,differentiation,histology types.

目的：探讨胰腺癌中vegf c、vegf d蛋白表达与淋巴结转移之间的相关关系，阐明癌周淋巴管增生在胰腺癌淋巴转移中的作用及意义。方法：免疫组化检测30例胰腺癌组织中vegf c、vegf d的表达及其与临床病理、淋巴结转移的关系。结果：胰腺癌vegf c、vegf d蛋白表达阳性率分别为73%(22/30)、57%（17/30），肿瘤周边部位显著高于肿瘤中心部位，其表达与肿瘤的部位、分化程度、组织学类型无关，与肿瘤的tnm分期有关，ⅲ~ⅳ期显著高于ⅰ~ⅱ期。

Then histochemical, electron microscopic, enzyme histo cytochemical and confocal laser scanning microscopic methods were employed to study the distribution of vinculin, cathepsin Dand nematolysosomes ...

结果在正常对照组神经元，组织蛋白酶D与纽蛋白分布于胞质及突起内；在CD及PMA处理神经元，纽蛋白及组织蛋白酶D的分布呈向心性移动，但集聚的部位不同；电镜酶细胞化学方法显示CD组及PMA组神经元内线状溶酶体均增多。

Objective To investigate the relationship between the infection of high risk-human papillomavirues and lung cancer in north of Henan province,and preliminarily definitize the role of high risk-HPVs in carcinogenesis of lung cancer.

采用聚合酶链反应方法检测肺癌组织中高危型HPVs的感染情况及分型，并分析相关高危型HPVs感染与肺癌组织学类型的关系。

Materials and MethodsMaterials; 10% formalin fixed, paraffin -embedded tissue samples, including ; MF 59 cases, neurodermatitis 20 cases, chronic eczema 20 cases, parap-soriasis 40 cases, all taken from laboratory of dermatopathology in No.

材料和方法实验材料：来自中国医科大学附属第一床医院皮肤科病理室，10%福尔马林固定、石蜡包埋组织标本：蕈样肉芽肿59例、神经性皮炎20例、慢性湿疹20例、副银屑病40例、临床及病理都符合其疾病的诊断标准；正常皮肤组织标本20例：来自中国医科大学附属第一医院整形外科手术切除的正常人正常皮肤。

However, the molecular mechanisms underlying premature aging up 8-MOP/UVA are as yet unknown. In our studies, dermis of the non-lesion back skin of vulgar psoriatic patients who had been treated by PUVA and the cultured foetus dermic fibroblasts were selected to be observed.

本课题以PUVA治疗寻常型银屑病患者背部非皮损区真皮及培养的胎儿真皮成纤维细胞为对象，采用HE染色、Verhoeff染色、电镜、酶组织化学、免疫组织化学、原位杂交、流式细胞计数仪、三引物PCR、PCR-SSCP技术及PCR产物直接测序等技术和方法，研究了8-MOP/UVA对皮肤光老化形成的影响及芳维A酸乙酯的拮抗作用。

Then clarify the role of apoptosis of BPH mechanism.Methods:Tissues of 25 BPH patients through TURP were embedded in paraffin,which cystourethroscope show those protate growed severely dissymmetric.

采用自身对照设计，应用免疫组织化学的方法，对caspase3蛋白在25 例不对称BPH中增生明显叶与不明显叶组织中表达水平进行检测。

METHODS The mechanism and condition of thrombus formation by copper needle were studied in vivo and in vitro using electrophysics, atom absorption spectrophtometry, histological, and histochemical methods.

以体外实验和大鼠体内实验为模型，用电子物理学、原子光谱吸收法、组织学和组织化学等方法研究铜针通电后血栓形成的机制和条件。

To observe the growth of embryon heart by histological method.

并通过组织胚胎学方法对胚胎及胎儿心脏组织发生进行研究。

To investigate the effects of moderate intensity endurance training (55%—75%VO 2max )on biochemical responses of diphragm,gastrocnemius and heart in normal and emphysematous rats, SD rats were randomly divided into two groups first.

在末次运动锻炼结束后 48～ 72小时处死大鼠，用组织化学方法染色，光密度分析测定膈肌肋部、腓肠肌和心肌纤维琥珀酸脱氢酶和乳酸脱氢酶活性，评价相应肌组织的氧化代谢能力。

Methods Rat spinal cord injury models were established by aneurysm clip compression, and nestin expression and proliferation of ependymal cells at different times were shown with pathological and immuno-histochemical staining.

应用动脉瘤夹压迫建立大鼠脊髓损伤模型，通过组织病理学及免疫组织化学方法检测不同时段室管膜细胞的反应性增生和神经外胚层多潜能细胞特异性抗原nestin的表达。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。