组织方法

It is timesaving and economical to extract mtDNA and nDNA simultaneously from the same tissues, which is a better way for DNA study.

同一组织中能同时提取mtDNA和nDNA，既节省组织又节省时间和费用，为DNA的研究提供了较好的实验方法。

Results: The result is that PCNA positive appeared 6 hours after transplantation, increase unit time pass by, and reach peat at 1 to 2 week. After 2 weeks, PCNA positive of SMC in the tunica media begin to decline and recover to the level 6 hours after the operation at the 8 weeks.

本实验的第三部分，我们将于液氮固定的移植静脉段经组织匀浆后用于研究ECE和ET-1的表达情况，我们分别从mRNA水平和蛋白质水平，采用RT-PCR和免疫组织化学方法，对ECE和ET-1在移植静脉的表达情况进行分析。

Methods Expression of TIG1 mRNA in normal tissues, uninvolved skin tissues and psoriatic lesions was detected by in situ hybridization.

用原位杂交的方法检测正常组皮肤组织、银屑病组未受累皮肤组织和皮损中TIG1m RNA的表达。

Methods:TIG2 protein and mRNA in normal skin tissues,uninvolved skin and SCC lesions were detected by immunohistochemistry and hybridization in situ.

用免疫组化和原位杂交的方法检测正常皮肤组织，未受累组织及鳞癌皮损中TIG2蛋白和mRNA的表达变化？

Intimal areas were measured using morphometric analysis of perfusion-fixed vein graft specimens, and intimal thickness was calculated using circumferential measurements. The SMC proliferation was studied by the immunohistochemical detection of proliferating cell nuclear antigen. Expression of MMP-2、MMP-9 mRNA in vein grafts and unoperated control Vein grafts was detected by reverse transcription polymerase chain reaction. Substrate gel zymography was used to determine the proteolytic activity.

分别行HE染色、Verhoeff弹性纤维染色观察组织病理变化，计算机病理图象分析系统测量新生内膜厚度及面积，免疫组织化学方法检测静脉壁增殖细胞核抗原表达以观察细胞增殖情况，半定量逆转录-聚合酶链反应检测静脉壁基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)mRNA的表达，明胶酶谱法检测MMP-2、MMP-9活性，比较各组之间的差别。

Methods Twenty-two adolescent SD female rats were selected. All rats were killed by dislocation of cervical vertebra, the eyeballs were in paraffin imbedding and made to a series of sections, using immunohistochemical method; ERα and ERβ distribution were investigated in uvea tissue of rats; and quantitied by Tanaka scores analytical method. The uteri of rats was used as positive control and PBS as negative control.

选择青春期SD雌性大鼠22只，采用颈椎脱臼处死大鼠，取眼球作常规石蜡包埋，连续切片， SP免疫组织化学方法显示ERα、ERβ在葡萄膜组织的表达；采用Tanaka记分法对ERα、ERβ进行定量分析，并设正常大鼠子宫作阳性对照；PBS代替一抗作阴性对照。

Determination of relative tumor vascularity in the tumor tissue using FITCdextran adsorption can be used as an antiangiogenic model in vivo.

和藻酸盐包被试验相比，FITCdextran测定肿瘤组织血管相对密度是一种简单有效的量化测定肿瘤组织血管生成水平的方法。

MethodsExpressions of SP and vasoactive intestinal peptide in tongue base tissues of 49 patients with OSAHS were analyzed by immunohistochemical staining.

方法通过免疫组织化学染色对49例OSAHS患者的舌根组织的P物质及血管活性肠肽的表达进行分析。

Methods 34 cases were invesigated under the light microscopic l e vel and were studied by immunohistochemical technics.

报告34例鼻腔圆形细胞恶性肿瘤，观察其病理组织学表现，用免疫组织化学方法标记肿瘤。

To take up the mRNA from 46 cases with brain glioma and 12 cases with brain contusion,the WAF 1/CIP 1 RNA are carry out re frans formation PCR.

方法取46例胶质瘤组织及12例脑外伤组织，提取其mRNA，用WAF1／CIP1作为模板进行逆转录PCR。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。