组织方法

By pathology examination, the atherosclerotic plaques were found at the intima in Group I.

VEGF在动脉粥样硬化组织中蛋白及基因表达的研究应用免疫组化方法，观察动脉粥样硬化组织中VEGF蛋白表达的变化。

Objective To expiore the pathogenic mechanism of hemorrhagic fever with renal syndrome.Methods 2-3 days old inbred Balb/C suckling mice were inoculated intracerebrally with tissue suspension of soft parate petechia of HFRS patients and the HFRS virus RNA was tested in brain, lung and kidney tissues of sucking mice by nested PCR.

目的 探讨肾综合征出血热的发病机理方法 2～3天的近交系Balb/C乳鼠，脑内接早期HFRS重症患者软腭粘膜出血点组织悬液，用套式聚合酶链式反应检测乳鼠脑、肺及肾组织中的HFRS病毒RNA。

Methods: 21 buttons of keratoconus, 7 buttons of normal cornea were detected.

材料与方法：取角膜移植术钻下的圆锥角膜组织21例，无眼病的尸体角膜7例，全部标本为石蜡包埋，免疫组织化学采用SABC法。

Methods Immunohistochemistry was utilized to measure the expression of AQP3 in tissue samples from 30 patients with seborrheic keratosis, 15 patients with Bowen′s disease, 32 patients with squamous cell carcinoma, 17 patients with malignant melanoma and 15 normal human controls.

应用免疫组织化学方法检测30例脂溢性角化病、15例Bowen病、32例鳞状细胞癌、17例恶性黑素瘤及15例正常人皮肤组织中AQP3的表达。

Objective To explore the effect of high serum concentration of bile acids on energy metabolism of liver.Methods Bile acids were continously infused into the Wistar rats to simulate the serum concentration change of patients with obsturctive jaundice.Serum bile acids concentration,arterial blood ketone body ratio and energy charge of liver were determined.Results Aterial blood ketone body ratio and energy charge of liver decreased with the increase of serum bile acids concentration.Conclusion High serum concentration of bile acids plays an important role in hepatic fumction injury caused by biliary obstruction.

目的 探讨高胆汁酸血症单一因素对肝脏能量代谢的影响方法经Wistar大鼠颈内静脉持续给予外源性胆汁酸，升高其外周血清中胆汁酸浓度，模拟阻塞性黄疸患者外周血清中胆汁酸的变化规律，观察腹腔动脉血酮体比值及肝组织能荷变化，时间一周结果随外周血清中胆汁酸浓度的升高，腹腔动脉血酮体比值、肝组织能荷均呈进行性下降结论高胆汁酸血症时肝脏能量代谢受损，表明在阻塞性黄疸患者肝功能损害中高胆汁酸血症起了重要作用

The effect of solution treatment on microstructure and mechanical properties of 7B04 aluminum alloy were studied by optical microscope,tensile test,X-ray diffraction phase analysis and refinement of lattice parameter of α matrix.

通过显微组织观察、拉伸力学性能测试、XRD衍射物相分析以及α基体点阵常数的测量等方法研究了固溶处理对7B04铝合金组织和性能的影响。

Methods The MAL gene expression in 45 cancer patients was detected with RT-PCR and semi-quantitative RT-PCR technique.

采用RT-PCR和半定量RT-PCR方法检测45例食管癌组织和癌旁食管黏膜组织中MAL基因的表达。

MATERIALS AND METHODS: The protein expressions of MMP-7 and MMP-9 were evaluated by immunohistochemistry in 18 normal villi. 49 hydatidiform moles, in which 8 were malignantly transformed moles.

材料与方法：采用免疫组织化学PV-6000法测定49例葡萄胎（其中8例恶变，列为葡萄胎恶变组）和18例正常绒毛组织中的MMP-7和MMP-9蛋白表达量。

In this paper, two methods of marbling score and grey space correlation matrix were used to analyze the image features of fat and no fat structures in chilled beef.

分别运用大理石纹和灰度空间相关矩阵两种方法对含脂肪组织和不含脂肪组织冷却牛肉的图像特征进行了分析。

From image of fat structure, the information on the distribution and quantity features of marbling score structure was gathered.

在含脂肪组织牛肉图像中提取了能反映牛肉大理石纹结构分布和数量信息的特征量，在不含脂肪组织牛肉图像中提取出 3个反映牛肉纹理粗细的特征量，并用线性回归方法分别建立了特征量与肉嫩度等级间的数量关系。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。