组织方法

Serial cryostat sections of five hearts and seven atria of rats were prepared.

利用荧光组织化学和组织学染色连续在同一切片上显示的方法，观察了5只大鼠整个心脏和7只大鼠心房的连续切片。

In this paper,a new method of using cyclic temperature difference for the viability evaluation was proposed,and preliminary experiments were performed to demonstrate the new approach.

本文提出了循环温差法测定生物组织活性的新方法，并进行了初步的实验研究，给出了定量评价生物组织活性的数学关联式。

On the basis of in-depth study of the OAI-PMH protocol, this paper analyses and compares the deficiency of existing index and classification methods, and proposes a clustering method to organize the mass metadata which is harvested by service provider of OAI-PMH. This provides a new effective way for the rational organization of the metadata in the service provider.

本文在深入研究了OAI-PMH协议的基础上，分析比较了现有的索引方式和分类方式的不足，提出了用聚类方式来组织OAI-PMH协议中服务提供者收割回来的大量元数据的方法，为服务提供者中元数据的合理组织提供了一种新的有效途径。

Methods］ A total RNA from 10 cases withovarian papillary serous cystadenocarcinoma and normal ovarian tissues were extracted by a single step method of gene chip. The cDNA retro?鄄transcribed from equal quantity mRNA derived from 10 cases with ovarian papillary serous cystadenocarcinoma and normal ovarian tissues. The cDNA strand was labeled with fluorescent Cy5 and Cy3(normal ovarian epithelial cells) as probes.

方法］ 采用基因芯片技术，按一步法分别抽提10例卵巢浆液性乳头状囊腺癌患者的癌组织和正常卵巢组织的总RNA并纯化mRNA；逆转录合成以Cy5荧光标记卵巢癌细胞和Cy3荧光标记正常卵巢上皮细胞的cDNA作为探针，在含有8 464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

It has long been known that the poor corrosion resistance of brass in certain environment is due to the phenomenon of dezincification.

采用显微组织分析和脱锌腐蚀实验方法，研究了不同铋含量对黄铜的微观组织以及黄铜脱锌腐蚀性能的影响。

The differential expression of the desired gene in the target tissue is essential for gene therapy.

目的基因在靶组织中的差异表达在基因治疗中很重要，一种方法是用组织特异性启动子驱动治疗基因表达。

In this paper, a new photon migration approach for modeling fluorescence in tissue is described in detail based on diffusive reflectance light Monte Carlo model.

该文在组织漫反射光MonteCarlo仿真的基础上，提出了一种新的荧光光子迁移方法，并由此实现了对组织自体荧光的仿真。

Results 1. For the normal group,20.2%(101/500) specimens were detected positive forHPV infection. 1512 women were included in the abnormal group and 523(34.6%)specimens were detected positive for HPV DNA, including 407(77.8%) one-typeinfection, 82(15.7%) diplex infection and 34(6.5%) multiple infection.

收集宫颈癌组织标本，针对HPV16亚型分别设计L1、E6和E7基因特异性引物，用PCR方法从宫颈癌组织中获得各基因的全长序列，并构建克隆载体pMD18-T，进行序列分析，同时分别以L1、E6和E7基因序列进行进化分析。

RESULTSdiabetic rats showed typical symptoms involving elevated serum glucose and weight loss (2)The rats in DM+VaD group reacted slower and made more error numbers in Y-maze than the rats in VaD group did, with a prolonged total reacting time in a whole day.(3)More neurons with serious damages, such as edema, ischemia or pyknosis could be found in DM+VaD group than in VaD group. More disarrangement of cone neurons, obvious glia proliferation, and more neuron apoptosis were found in CA area of hippocampus. CONCLUSIONS (1)2-VO in STZ-induced diabetic rats succeeded in establishing VaD animal models.(2) The cognitive dysfunction induced by VaD rats had been deteriorated by diabetes mellitus.(3)Morphology evidences proved that diabetes mellitus aggravated brain damages induced by VaD.PART II Effect of diabetes mellitus on the cholinergic nervous systemin CA1 area of hippocampus of VaDOBJECTIVE To estimate the role of cholinergic nervous system in hippocampus during the process of cognitive dysfunction aggravated by diabetes mellitus in DM+VaD group. METHODS Observe the changes of ChAT protein by immunohistochemistry stain. Chose three rats randomly from each group at each pointat two week, four week, and eight week, then sepa

经腹腔注射链脲佐菌素诱导慢性实验性糖尿病，1周后永久性结扎双侧颈总动脉(2-VO)制作VaD模型记录术后2周、4周和8周各组大鼠的体重及血糖；应用Y-迷宫检测空间定向学第二军医大学博士论文中…丈摘…要1ia；一色恤~﹁染澎糕行HE染色观察组织病理学孪叱；胶质细胞原纤维酸性蛋白protellZ，Cf冰尸标记观察星形胶质细舱的激活情况、橄声软公点娜育票票众糯黑橄居端粗默孺篇筑价井袱第二部分糖尿病对血管性痴呆大鼠海马c心区胆碱能神经系统的影响探讨海马CAI区胆碱能神经系统在糖尿病加重确D一大鼠认知障碍中所实验动物及分组同第一部分，应用免疫组织化学染色方法现察海马CAI{介狱牛第晕军l袭拔常博粗{论义

Courses combine theory and practice through teaching methods so that students master the incoming supply organizations, professional knowledge and expertise to use these characteristics to analyze the incoming market, know how to market supply and demand forecast survey, how to organize supply , how to make freight plans; control incoming commercial business and operations, total revenue costs, freight quality management expertise to apply this knowledge in an integrated shipping companies, port terminal operators, cargo handling companies, freight forwarders, warehousing companies, incoming logistics companies, business service management companies related to cargo management, port-site management, cargo handling operations, cargo transfer agency business operations and management of import and export of container cargo business operations and management, port yard management, transport business management , dangerous goods transport business operation and management, domestic water transport document operations, cargo handling accidents and customer service.

课程通过理论与实践相结合的教学方法，使学生掌握港航货源组织的专业知识，能够运用这些专业知识去分析港航市场的特点，懂得如何进行货源的市场调查与需求预测，如何进行货源组织，如何制定货运计划；掌握港航商务业务与作业、费用计收、货运质量管理的专业知识，能够综合运用这些知识在船舶运输公司、港口码头公司、理货公司、货运代理公司、仓储公司、物流公司等港航商务业务管理相关的公司进行货运管理、码头现场管理、理货作业、货运代办中转业务的操作与管理、集装箱货物的进出口商务作业与管理、港口库场管理、联运业务管理、危险品运输业务的操作与管理、国内水运的单证操作、货运事故的处理、客户服务等。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。