组织方法

Methods: Immunohistochemical method was used to observe the distribution of LN and FN in normal chorion, hyditidiform mole and choriocarcinoma.

用免疫组织化学方法观察层粘连蛋白和纤维粘连蛋白在正常绒毛膜、葡萄胎和绒毛膜癌组织中的表达。

Methods 105 cases of GTD tissues and 20 cases of normal chorion villi specimens were examined by immunohistochemical techniques using EGFR monoclonal antibody.

采用免疫组化方法，检测石蜡包埋的105例妊娠滋养细胞肿瘤组织和20例正常早孕绒毛组织EGFR蛋白表达水平。

With the corm and scale of A. albus in Zigui County in Hubei Province as tested material, MS was used as the basic medium with different hormones to respectively match 10 kinds of media for induction, differentiation for tissue culture of A. albus The induced clumpy buds were cut into single plants and were put into the rooting medium of MS+NAA 0.1-0.5 mg/L and the effects of different hormone matching on callus formation, bud differentiation and rooting were studied.

方法］以湖北省秭归县的花魔芋球茎和鳞片作为外植体，以MS培养基为基本培养基，添加不同激素，分别组配成10种诱导培养基和分化培养基进行组织培养，然后将诱导的魔芋丛芽切成单株后接入MS+NAA0.1~0.5 mg/L生根培养基，研究不同激素配比对愈伤组织形成和芽分化以及生根的影响。

Immunohistochemical staining was used to detect the protein levels of Bcl-2 and Bax in the cochlea after 3 months.

在成模后3个月时采用免疫组织化学方法，对大鼠耳蜗组织进行Bcl-2和Bax的表达检测。

Objective To explore a new method of cochlear sectioning to get high qualitative cochlear section catering for immunohistochemistry of cochlea.

目的 探索一种新的耳蜗组织切片方法，以获得高质量的切片，满足其免疫组织化学染色的需要。

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

The functional imbalance between the normal and abnormal side may bring about dislocation and malformation of the base of nasal columella as well as the septal structures.

另10例患者术中经鼻小柱中下1/3处横切口，做冠状薄层组织全层切除，10%福尔马林固定，组织学观察方法同前。

The dissertation,under the guidance of historic materialism and by means of combiningtheoretical and positivist analysis,employs the method of analysis in institutional economics so as todissect the composition and interaction of social forces and displays the changes of social structure andthe process of social institutions in rural China of different periods.

论文以历史唯物主义为指导，借鉴制度经济学的分析方法，通过理论分析和实证分析相结合，深度剖析不同时段（以公社体制突破后为重心）乡村中国的社会力量组合和交易情形，动态地展示乡村中国的社会结构变动和社会组织演进过程，力图揭示乡村组织演进中的困境和走出困境的路径。

This dissertation consists of the following eight chapters: the first chapter is the guiding part of the thesis, which introduces the study background of this dissertation, and expounds the theoretical and practical significance of HeHe Management, writing framework and research method; the second chapter is the literature summarizing part, which expounds some important concepts related to HeHe Management including"ren wei"(self-cultivation),"wei ren","ren wei wei ren"(considerateness-oriented) and"HeHe", and summarizes the related literature; the third chapter systematically constructs the theoretical foundation of HeHe Management, and explores the support of HeHe Mangement in terms of philosophy, culturology and management; the fourth chapter analyses the relation among"ren wei"(self-cultivation),"ji shen he he" and self-management, and expounds the functional mechanism in psychology, main content and realizing approaches of self -management; the fifth chapter analyses the relation among"wei ren","qun ji he he" and"guanxi"management, and expounds the functional mechanism, main content and realizing approaches of"guanxi"management; the sixth chapter analyses the inherent relation among"ren wei wei ren"(considerateness-oriented),"zu zhi he he" and HeHe Management, expounds the theory of HeHe Management and its mode under the perspective of"ren wei wei ren"(considerateness -oriented) of Eastern Management, and discusses the value viewpoint of HeHe and the construction of corporation HeHe culture; the seventh chapter expounds the applying target, applying means and applying space of HeHe Management, and explores the practices of HeHe Management in corporational organizations by the way of case study from multi-layers and multi-angles; the eighth chapter expounds the new development of contemporary managerial practices and theories, explores the new tendency of contemporary management development, i.e.

本文共分八章：第一章是论文的导论部分，介绍本文的研究背景，阐述和合管理的理论与实践意义、写作框架和研究方法；第二章是文献综述部分，阐述包括"人为"、"为人"、"人为为人"与"和合"在内的与和合管理有关的重要概念，对相关文献进行综述；第三章系统构建和合管理的理论基础，探寻和合管理在哲学、文化学、管理学方面的支撑点；第四章分析"人为"、"己身和合"与自我管理的关系，阐述自我管理在心理学上的运行机制、主要内容及其实现途径；第五章分析"为人"、"群己和合"与关系管理的关系，阐述关系管理的运行机理、主要内容及其实现；第六章分析"人为为人"、"组织和合"与"和合管理"的内在关系，阐述东方管理"人为"观下的和合管理理论及其模式，探讨和合价值观及企业和合文化建设；第七章阐述和合管理的应用目标、应用方式及应用空间，通过案例研究的方式多层面、多角度探讨和合管理在企业组织中的实践；第八章阐述当代管理实践与管理理论的新发展，探讨当代管理学理论发展的新趋势——东西方管理的融合与发展，认为和合管理是管理理论发展到哲学阶段的产物，是企业管理的新发展。

It was indicated that the highlevel expressions of PCNA and VEGF might corporately accelerate the progression of gastric cancer.

本研究旨在利用免疫组织化学染色方法检测正常胃和胃癌组织中PCNA、VEGF的表达，探讨二者胃癌发生、发展中的作用。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。