组织方法

MTT method was used to detect the proliferation of the cells. Cell alkaline phosphatase activity and expression of collagen type I were assessed.

另以组织化学和免疫组织化学方法检测传代培养的SD大鼠骨髓基质成骨细胞在PMMA驻极体表面的增殖和分化情况。

By means ofthe analytical method of institution transformation and cost-benefit of thenew-institutional economics,the author makes deliberate studies on theseinducing factors from the emergence and transform of the industrialorganization and the bank organization.

作者运用新制度经济学有关制度变迁、成本收益的分析方法，重点从产业组织和银行组织产生和变迁的角度对这一诱因进行了深入考察。主要结论和创新是

1、The Entropy、Smoothness、Uniformity and Relation (base on co-occurrence)are significantly difference between the patients and volunteers(p.05).2、The Contrast (base on co-occurrence matrices) and Distance (based on autocovariance coefficients) tend to be higher volunteers, but not reaching statistical significance.3、Among all the thirty-nine features, the most significant difference was obtained using Relation with the pixel distance varied from 1 to 3 pixels.

本实验使用纹理分析的方法研究正常膀胱壁组织和膀胱赘生物组织的纹理特征，并筛选出四种存在显著统计学差异的纹理特征，为后续利用纹理特征研究膀胱肿瘤浸润肌层深度提供了依据。另外，如前所述，目前膀胱肿瘤的诊断主要依靠膀胱镜，但作为一种侵入性操作，膀胱镜并不适合人群疾病普查和患者的治疗后随访。

The battel for presidential nomination is even more unpredictable of the stage ,former Akansa governor Mick continue to surge in Aiwaa and other early contest state south,states ,Kakabi's rise maybe come after of former New York city mayor Juli Ania ,July Anian contiune to lead among most republican's most national polls ,but traill behind the early primary states 's of Aianwa , July Anian continue to enphasize on the tough pose to terrorist ,involed to take hardline to against the prospect of the Iran to developing a nuclear weapon capabilty , he was interviewed in a press programm ,the generary thied voting Aiawa will begins a conpressed monthes of talkes and primary , ewhen more than 20states will vote for the presidential presisi in both parties the nominatee for the both parties will be confirm to the the party conventions later in a years

中情局人员被怀疑在审问过情中使用了严酷的审问方法。中情局局长在一封致全体CIA员工的信中说，这些录像对CIA职员的安全造成严重的威胁。录像带有可能暴露CIA人员的身份，CIA职员及其家属可能会受到基地组织及其家属的报复。民间自由组织和一些资深政治家不接受CIA的解释，他们指出，CIA曾被要求上交录像带。比如在911事件事，他们否认录像带的存在。

Primers used in PCR were designed based on the sequence of mitochondrial 16S rDNA (AJ250642 and AF312718) and beta-actin (AY910691) of Eriocheir sinensis which had been deposited in GenBank. PCR analysis using total DNA from muscle, branchia, testis and sperm indicated that 16S rDNA exhibited different expression in the four samples. Beta-actin gene was detected plentiful in all the four tissues/cells and exhibited the same expression pattern. 16S rDNA gene amplification was detected at high levels in muscle and branchia, appreciable low in testis and very low in sperm. The PCR products of 16S rDNA gene were sequenced and aligned with the sequence of 16S rDNA from GenBank (AJ250642 and AF3 12718), and the alignment result showed there was no difference between them.

通过GenBank中的中华绒鳌蟹线粒体16S rDNA序列设计引物，利用PCR扩增方法，以beta-actin做内参，检测了中华绒螯蟹肌肉、鳃、精巢和精子中线粒体16S rDNA扩增情况，发现各组织或细胞beta-actin扩增片段大小、条带宽度和亮度基本一致，表明各模板DNA量基本一致；而在同一DNA浓度下，16S rDNA的扩增片段长度虽一致，但其产物量存在明显差异，精子16s rDNA产物量显著低于其他3种组织，其条带宽度和亮度很弱；16S rDNA扩增产物经测序分析及比对证明与已有序列完全吻合。

Adopting orthogonal polynomial regression method,the mathema tical model of the effects of NAA on bulbil induction, and that of 2,4ˉD on callus induction f rom leaflet on oriental lily cv."Tiber" were established.

借助正交回归多项式建立数学模型的方法，研究了NAA对东方百合"蒂伯"鳞片诱导小鳞茎及2，4ˉD对小叶片诱导愈伤组织的影响，以探讨东方百合组织培养中NAA及 2，4ˉD 的用量范围及最佳用量。

METHODS: Autologous whole blood was injected into the vitreous cavity of albino rabbits after canalization, thus induced experimental PVR model. Concentration of PDGF.

采用眼穿通伤+注血法制备PVR模型，进行眼底及常规组织病理学观察，ELISA法检测玻璃体中血小板源性生长因子的浓度，用免疫组织化学方法观察增生膜与血管内皮细胞表面血管细胞粘附分子-1（VCAM-1）及细胞间粘附分子-1（ICAM-1）的表达。

In this paper, callus culture, derived from leaf or petal, and capitulum culture combined with irradiation of γ-ray for mutation were introduced.

菊花有30%品种为变异种，且菊花组织培养之分化与逆分化非常容易调控，因此本文介绍利用叶片或花托癒伤组织、或花托培养等方法，配合γ-射线照射进行诱变育种。

Methods:stimulating the animal model of rats with the experimental prostatitis by injecting carrageenin into rat prostates.We compared the model group with other groups which had taken Nanyanxiao granule,and made pathological examination of prostate.

采用向大鼠前列腺组织内注入角叉菜胶的方法复制大鼠前列腺炎模型，并分别对模型组及口服男炎消冲剂后的大鼠组进行前列腺组织病理学检查。

The invention provides a method for producing transgene sugarcane quickly with pmi genes. The method primarily comprises the steps of establishing a mannitose screening system; transformation of a gene gun; acquisition of resistant callus; differentiation and screening of the resistant callus; acquisition and rootage of resistant plantlets; molecule detection of resistant plantlets regenerated and detection of pmi gene expression by the chlorophenol red detection method.

一种利用pmi基因快速获得转基因甘蔗的方法，主要内容包括：甘露糖筛选体系的建立，基因枪转化和抗性愈伤组织的获得，抗性愈伤组织的分化筛选，抗性苗的获得和生根，抗性再生苗的分子检测和氯酚红法检测法检测pmi基因表达。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。