组织方法

Extract total proteins from these two tissue samples respectively and then employ a SDS-PAGE method to identify the most differentially expressed protein between them and estimate the molecular weight and relative abundance ratio of the protein.

从这两个组织中分别提取总蛋白并定量，然后采用 SDS-PAGE 方法识别出在这两个组织中差异表达最为明显的蛋白质并且估计它的分子质量以及相应的丰度。

a simple and stable method for isolation and cultivation human adipose-derived adult stromal cells is successfully established , which provides the foundation for future usage of human adas cells in tissue engneering.

我们建立了一种自人体脂肪组织分离、培养人脂肪基质细胞简便稳定的方法，为其应用于组织工程提供了基础。

A bioselective membrane electrode sensistive to adrenine has been prepared and made in flow type by means of banana tissue covering an oxygen electrode.

将香蕉组织偶合氧电极研制成肾上腺素生物组织电极，并装制成流通型式。文中对此电极的响应特性进行了研究，探讨了各种实验条件及有关参量的影响。并将电极用于肾上腺素原粉的含量测定，结果与标准方法基本一致

Rt-PA(5mg/kg) dissolve bolt gives treatment after the 3h that be short of blood, associated group gives vein injection at the same time Yidala is acted according to (the 24h after 3mg/kg) dissolve bolt undertakes nerve function grading and cerebral stalk die concentration percentage by volume leads an analysis, determine blue content of the thread of ideas in writing of the Yi in cerebral tissue, with immune organization chemical method evaluates dissolve bolt afterbrain to organize the change of hematic head protective screen.

缺血3h后给予rt-PA(5mg/kg)溶栓治疗，联合组同时给予静脉注射依达拉奉(3mg/kg)溶栓后24h进行神经功能评分和脑梗死体积百分率分析，测定脑组织中伊文思蓝含量，用免疫组织化学方法评估溶栓后脑组织血脑屏障的变化。

The paper mainly regards western economic theory as guidelines, using the basic method that the theory combines with reality, systematically studies the relationship between Agricultural Industrial Organization and System of Agricultural Cooperation, emphatically describes the function of the Innovation of SAC during the optimization to AIO.

论文主要以西方经济学理论为指导，运用理论与实际相结合的基本方法，系统地研究了农业产业组织与农业合作制度的关系，着重论述农业合作制度创新对农业产业组织优化的作用机理。

Methods The expressions of survivin albumen in 50 cases of gastric cancer and 20 normal people were detected immunohistochemistry method and its relation with the clinical-pathological parameters of gastric cancer was analyzed.

方法应用免疫组化法检测Survivin蛋白在50例胃癌组织和20例正常胃黏膜组织中的表达情况，并分析Survivin蛋白与胃癌临床病理指标的关系。

To obtain DNA noninvasively from the silkworms Bombyx mori and Philosamia Cynthia ricini, we collected the sloughs of fifth instar and the wings of moth, and then extracted DNA by SDS and the DNA extraction kit of animal tissue and purified them.

对家蚕和蓖麻蚕的蚕蜕和蛾翅分别采用SDS裂解法和动物组织基因组试剂盒提取法提取DNA ，并与常规方法以蚕蛹提取的DNA一起进行RAPD扩增图谱比较、分析，结果表明用非损伤性方法提取的DNA的RAPD图谱和用常规方法提取的DNA的RAPD图谱基本一

This article aims to explore the basic principles and methods in writing online learning resources. It is divided into four parts. The first part introduces the characteristics and significance of online learning resources. The second part elaborates the basic principles in terms of choice, writing, organization, layout, presentation of the learning content. The third part describes how to write online learning resources including use of language, rubrics and graphics, etc. The fourth part describes the uses of the principles through a case study.

本文的目的是介绍和探讨网上学习材料撰写的基本原理和方法，文章分为四个部分：第一部分说明网上学习材料撰写的特点和重要性；第二部分阐述网上学习材料撰写的基本原理，从学习内容的编排、学习内容的撰写、学习内容的选择、学习内容的组织、学习内容的呈现五个方面进行了分析；第三部分描述网上学习材料撰写的方法，包括语言使用、动词使用、图表使用等；第四部分通过案例，描述网上学习材料撰写的原理和方法的实际应用。

This paper gives several methods to validate these new methods by analyzing images of stage micrometer, metallurgical sample, and images that consisting of definite area in geometric graph, Several kinds of validation methods including of traceability of a quantity value, printing microstucture graph, comparing microstucture photo with standard photos, length meansurement, and area meansurement are discussed in this paper.

通过分析显微镜刻度尺图像、金相样品图像，及给定特定图形且已知面积的图像，依据ISO/IEC 17025检测和校准实验室能力的通用要求，并结合金相检验标准，阐述了金相显微镜利用新方法的方法确认，其中包括量值溯源、金相组织输出、图谱比较、长度类测量和面积类测量的确认方法。

At the same time, pattern recognition has been used for abstracting characteristics、predicting the life and evaluating the properties of engine. This research contributes toward global diagnosis for the diesel engine in eight ways.(1) Put forward"the Arithmetic of distinguish the period automatically using auto-correlation method";(2) Separate the waves of continuous knocks using WV distribution;(3) Propose"the logarithmic spectrum approximate method"to analysis the response of the knock between the steel and steel;(4) Discuss the relationship between the time-frequency distribution and timescale distribution in detail, and give the principle of classification;(5) Use the wavelet method to analysis the pressure signal of exhaust、suction;(6) Use the shape of vibration signal to analyse the working situation of cylinder;(7) Use the pattern recognition to select the good samples and abstract the effective characteristics;(8) Develop the model of total properties evaluation and life prediction.

本文在广泛的、深入研究的基础上，应用多种信号处理手段来分析处理数据，同时利用模式识别方法对故障的特征提取、寿命预测以及性能评价作了探讨，本文进行了以下几项工作：（1）提出了基于自相关自动周期识别算法的柴油机振动信号预处理策略；（2）提出了基于WVD分布的连续撞击响应信号的分离策略；（3）提出了基于对数谱拟合方法的钢—钢撞击响应求解模型；（4）提出了基于波形分析的非正常燃烧振动信号的诊断模型；（5）提出了基于小波细节信息的进气压力信号和曲轴箱压力信号的诊断模型；（6）提出了基于小波近似的喷油正时信号中燃烧位置的自动定位；（7）提出了基于统计模式识别方法的故障诊断知识的自学习与自组织策略；（8）提出了基于特征压缩思想的柴油机总体性能评价及寿命预测模型。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。