组织方法

As we all known, with the founding of Euclidean geometry in ancient Greece, with the development of analytic geometry and other kinds of geometries, with F.Kline" s Erlanger program in 1872 and the new developments of geometry in 20th century such as topology and so on, man has developed their understand of geometry. On the other hand, Euclid formed geometry as a deductive system by using axiomatic theory for the first time. The content and method of geometry have dramatically changed, but the geometry curriculum has not changed correspondingly until the first strike from Kline and Perry" s appealing.

纵观几何学发展的历史，可以称得上波澜壮阔：一方面，从古希腊时代的欧氏综合几何，到近代解析几何等多种几何的发展，以及用变换的方法处理几何的埃尔朗根纲领，到20世纪拓扑学、高维空间理论等几何学的新发展，这一切都在不断丰富人们对几何学的认识；另一方面，从欧几里得第一次使用公理化方法把几何学组织成一个逻辑演绎体系，到罗巴切夫斯基非欧几何的发现，以及希尔伯特形式公理体系的建立，极大地发展了公理化思想方法，不管是几何学的内容还是方法都发生了质的飞跃。

In this study, the classification of Chinese Babesia spp. infective to cattle were carried out by the classical methods and molecular biotechnology, and investigated the vector ticks of an unidentified Babesia sp. isolated from Xinjiang province by the experimental transmission, and observed the developmental forms of Babesia sp. in the engorged Hyalomma anatolicum anatolicum females;We developed the indirect hemoagglutination test for Babesia bigemina, and evaluated its effect in the sero-survey;We made slow-released injection of imidocarb, and estimated its efficacy on the treatment and prevention of Babesia bigemina infection by the laboratory work and the field tests. The major results were presented as following:1. Discovery of a bovine Babesia sp.

本研究以牛的巴贝斯虫为研究对象，采用传统技术与分子生物学技术相结合的方法对在我国已分离的牛巴贝斯虫病主要病原进行了分类学研究，在实验室内运用蜱传播试验对分离于我国新疆的牛的巴贝斯虫未定种的媒介蜱及其传播方式进行了详细的研究，采用组织抹片方法对牛的巴贝斯虫未定种在小亚璃眼蜱饱血雌虫体内的发育过程进行了观察；建立了间接血球凝集试验诊断方法，并对其在血清学调查中的应用效果进行了考察；研制了咪唑苯脲缓释注射剂，并通过实验室工作和田间试验对防治效果进行了评价。

This article examines the dairy industry in the light of the analysis frame of the industrial chain with industrial organization\'s SCP analysis model, the game theory analysis method, the value chain analysis method, the case analysis and the econometric analysis method and has carried on a systematically theoretical discussion of and the empirical analyses of five key links of the longitudinal dairying industry chain and their cooperation mechanisms.

本文把奶业纳入产业链的分析框架，运用产业组织的SCP分析范式、博弈论分析方法、价值链分析方法、案例分析和计量经济分析方法对乳业纵向产业链的五个主要环节及其协作链接方式进行了系统的理论探讨和实证分析。

For many years,the training of nurses just stops at the procedures of planning,task assignments and examinations,at performance of administration,restriction and forcing and at forced-feeding teaching method in the full course for the routine training courses so that the training methods are dull and ineffective.

多年来医院对护士的训练大多停留在制订计划-布置任务-组织考核等几个环节上，全程实行管、卡、压，方法比较陈旧，做法多是满堂灌，内容基本是常用科目。教育训练的方法不新颖，缺乏吸引力，效果不佳。针对其薄弱点，对教育训练的方法进行了改革。

To evaluate the safety of the DNA vaccine pVAX1/F1-V against plague, the purity of plasmid DNA pVAX1/F1-V was detected by SDS-PAGE and gelose electrophoresis method; pasmid DNA was detected by PCR in tissues of BALB/c mice immunized with the plasmids intramuscular injection.

为了研究鼠疫DNA疫苗pVAX1/F1-V质粒的安全性，试验运用SDS-PAGE电泳和琼脂糖电泳等方法检测了鼠疫DNA疫苗pVAX1/F1-V质粒的纯度，并以小鼠为动物模型进行了质粒pVAX1/F1-V的急性毒性和长期毒性试验，用PCR方法检测外源基因在小鼠组织中的分布情况，用ELISA、ELISPOT方法检测了其自身的免疫反应。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段，应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段；在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法；检测方法的特异性试验结果表明，该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应；检测方法的敏感性试验结果表明，通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg；对20份临床样品检测中，有10份为AIV阳性，其中有5份为H5亚型病毒阳性。

Direct difference is sensitive to noisy data, so a threshold limitation is given, which can not only filter the result from noisy data but also pick edge points up according men's desire. On the other hand, artificial intelligence is used for segmentation in reverse engineering. Because the essence of segmentation is clustering similar characteristics and self-organizing feature mapping is one function of neural network, SOFM network is used for segmentation in the thesis, coordinates and normal of data points is took as the characteristics vector.

第二种方法，基于双向角度微分的边点提取法，针对三维正交模型采用基于截面线的方式组织数据，此方法比基于曲率半径方法更简洁，不用进行曲面拟合，大大提高了计算速度；计算过程中由数据点的直接差分逼近微分，但直接差分对数据误差敏感，为此，本文提出了阈值过滤的方法，一方面过滤了数据误差在计算结果中引起的噪音，另一方面可以按照设计意图控制边点的提取；来决定是否提取光滑交接的边点。

The study at the undergraduate stage will provide students with strong backgrounds in theoretical fundamentals and systematic expertise of materials science and engineering, help students learn about the trend of development in this field, master necessary experimental and computational methods, skills, and have a good command of a foreign language, educate students to develop engineering problem solving skills or enable them to develop new materials, products, processes, and equipment, and capacitate them to sort out problems in practical applications by methods of analyzing chemical composition and structure of materials, controlling material manufacturing process and quality, and testing and analyzing material properties, and by techniques of synthesizing materials and processing material products.

学生通过本科阶段的学习，牢固掌握材料科学与工程学科理论基础和系统的专门知识；了解本领域的发展动向；掌握必要的实验、计算方法和技术；精通一门外国语；具有解决工程问题或从事新材料、新产品、新工艺、新设备的开发能力；能运用材料化学成份和组织结构的分析方法、材料的制造过程和质量控制方法、材料性能检测和分析方法、材料的改性技术、材料制品的加工工艺和技术等解决实际应用问题。

RT-PCR was a sensitive and stable method. It would be useful for large-scale diagnosis.A length cDNA of CEVd had been cloned into plasmid vector. Biotinylated probes were prepared from these plasmid DNAs. CEVd were examined from the positive and netative materials by Dot-blotting hybridization and imprint-hybridization. The result was exposed to x-ray film. Singles were detected for positive materials spots probed with probe, indicating that under the reaction conditions the probe hybridized with the target viroid specifically.

核酸杂交法检测：利用已经克隆的美国柑桔裂皮类病毒cDNA序列，制备生物素标记的核苷酸探针，采用斑点杂交的方法和组织印迹的方法分别对阳性材料进行杂交检测，通过胶片曝光显示杂交信号，发现在两种方法的检测结果中阳性材料都有强的反应，而阴性材料无反应或较弱，该方法安全可靠、快速准确，适合大量样品的检测。

Methods The expression of TP and MVD was detected by immunohistochemistry method.

采用免疫组织化学方法检测 2 5 1例癌组织和相对应的 92例正常组织TP和微血管密度的表达。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。