组织方法

objective to investigate whether hypoxia preconditioning exist in adipose tissue,and construct the hypoxemic model of adipose tissue.methods after injected different density cocl2 to rat's subcutaneously of groin,use immunohistochemical method detect hif-1α protein,and use rt-pcr detect hif-1α mrna.results after injected different density cocl2,the adipose tissue of rat's groin express different degree of hif-1αprotein and hif-1αmrna.,and reach the peak when the density is 20-40 mg/kg and the time is 3 h.conclusion hypoxia preconditioning exist in adipose tissue,and the effect of preconditioning is most evidence when the density is 20-40 mg/kg and the time is 3 h.

目的 探讨脂肪组织中是否存在缺氧预适应现象，构建cocl2致缺氧的缺氧模型。方法不同浓度的cocl2大鼠腹股沟区皮下层浸润注射，分别用免疫组织化学法和rt-pcr法检测该区脂肪hif-1α蛋白和hif-1α mrna的表达情况。结果用不同浓度的cocl2行化学缺氧后在不同时间点观察有不同程度的hif-1α蛋白和hif-1α mrna表达，其中，在浓度为20～40 mg/kg，时间为3 h时脂肪组织中hif-1α表达达高峰。结论脂肪组织存在缺氧预适应现象；cocl2的浓度选择20～40 mg/kg，时间选择3 h时缺氧预适应效果较佳。

Methods: The expression of P57(superscript kip2) was examined in proliferating, involuting human hemangiomas and normal skin tissues by using immunohisto-chemical technique. Immunohistochemical technique for factor Ⅷ-related antigen was used to identify that the cells which expressed P57(superscript kip2) were endothelia.

采用免疫组织化学方法检测人皮肤血管瘤增生期、退化期及正常皮肤组织中P57(上标 kip2)的表达水平，并结合第Ⅷ因子相关抗原的免疫组织化学染色证实表达P57(上标 kip2)的细胞是血管内皮细胞。

Breast tissues were taken at surgery from patients with breast adenoma, nodule of lobuli glandulae mammariae and breast cancer. Normal samples adjacent to breast cancer were used as control. NIS mRNA and protein were assessed in these samples by reverse transcription polymerase chain reaction , Western blot and immunohistochemistry methods.

1。乳腺组织取自乳腺纤维瘤、乳腺小叶增生以及乳腺癌患者，另取癌旁正常乳腺组织作为对照，分别运用半定量逆转录聚合酶链反应、Western Blot方法和免疫组织化学技术检测NIS的基因和蛋白表达情况。

These animals were killed after 24 hours of treatments and instillation of meconiums. The measurements included lung tissue wet/dry ratio,lung injury score,the expression and level of AngⅡ,lung myeoperoxidase activity and TNF-α level.

模型制备后24h取材，观察其肺组织病理损伤并检测湿/干重比及进行病理评分；检测AngⅡ在肺组织的表达情况及用图像分析方法检测其含量；测定肺组织中肿瘤坏死因子-α的含量及肺匀浆髓过氧化物酶活性。

Methods Immunohistochemistry and double-immunofluorescence techniques were used to detect the expressions of MMPs and TIMPs in 5 normal prostate and 34 prostate carcinoma tissues.

采用免疫组织化学、免疫荧光双重染色、形态定量分析方法对5例正常前列腺组织和34例前列腺癌组织MMPs及TIMPs进行定性、定位、定量检测。

Fifteen one-layered fabrics with different texture, density of fabric and fiber disposition were designed and woven before the designation of medical barrier fabrics.

在设计医用屏蔽织物之前，设计与试织了十五种具有不同织物组织、经纬密度和原料配置的单层织物，通过对它们进行性能测试和分析，探讨织物组织结构对其拒液性和透湿性的影响，得出了织物组织、纤维细度、织物紧度、织物达到高密的方法等几个因素影响这两种性能的主要规律，为医用屏蔽织物的开发奠定了理论基础。

The foundational method to construct Command and Control Relationships Network Model is discussed.Entity elements of organizations are divided into Scouters,Decision Makers and Actors.Two dimensions of the power,"influence" and "domination",are studied in the point of sociological view,the conception of C2 relationships is redefined.

研究了构建C2组织的指挥控制关系网络模型的基本方法，将组织的实体要素划分为侦察单元、决策单元和执行单元三类；从社会学的角度分析了C2组织中指挥控制权力的&影响&和&支配&两个维度，并重新定义了指挥控制关系；将实体单元和单元之间的指挥控制关系抽象为网络模型的点和边。

Using immuno-histochemical, tissue cultural and immuno-electron microscopic methods, based on nerve-endocrine-immune net, the dynamic changes of 4 brain-gut peptides and 6 inflammation factors of excised saccalus rotunds of rabbit stimulated by germs, LPS, exotoxin were observed.

本研究以神经-内分泌-免疫网络学说为依据，运用免疫组织化学的方法，结合组织培养技术、免疫电镜技术等，对离体兔圆小囊受到大肠杆菌等4种细菌、内毒素、外毒素等抗原刺激后，其组织内的4种脑肠肽和6种炎症相关因子表达量的动态变化进行了系统观察研究。

MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标：应用光学显微镜、电子显微镜观察脊髓形态的变化，采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌，以改良的Gale联合行为评分法对脊髓功能进行评定，结果：免疫组织化学检测显示，嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度，延缓白质神经纤维的减少，促进髓鞘的修复与再生。

Each 1 μL site of spinal cord receiving 109 /L OECs at the speed of 1μL/ min. MAIN OUTCOME MEASURES: Morphology change of spinal cord was detected by light microscope and electronmicroscope, secreted brain-derived neurotrophic factor was measured by immunohistochemistry and PT-PCR, and the combine behavioral score was used to assess the functional recovery of spinal cord. RESULTS: Immunohistochemistry result showed that OECs transplantation could partly reduce the number of apoptosis cells in spinal gray matter, delay cells reduction in spinal white matter, and accelerate the medullary sheath repair and regeneration.

主要观察指标：应用光学显微镜、电子显微镜观察脊髓形态的变化，采用免疫组织化学、PT-PCR 的方法检测脊髓组织脑源性神经营养因子的分泌，以改良的Gale联合行为评分法对脊髓功能进行评定，结果：免疫组织化学检测显示，嗅鞘细胞移植能部分改善大鼠脊髓灰质神经细胞的凋亡程度，延缓白质神经纤维的减少，促进髓鞘的修复与再生。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。