组织方法

This topic investigate the change of epidermic cell in suspension and in simplex after they were preserved in defferent ways. The junction between cells and the adhesion between cells and matrix have important roles in the hypothermic impairment of tissue.Their existence affect the recovery of cells under hypothermia. Consequently,the cytoskeleton system may contribute to the impairment caused by junctions.

本研究利用体外培养的表皮细胞单层和单细胞悬液，采用不同的保存方法进行保存，观察到组织细胞间的连接以及细胞与细胞基质的连接对组织冷冻的损伤有重要的作用，它们的存在直接影响组织细胞的活力，其原因与细胞骨架系统有关。

To obtain precise result, itis necessary, in the aspect of hardware, to set up one stable optics system with highSNR, only if so, can we do the measurement with the steady NIR. To do so, we designthe optics system using AOTF, and take some measures to increase the light energyand the system SNR, which includes the three layers structure of the light chamber,the equipments to cover the useless light, polarizing to reduce the influence of 0 gradelight and focalize the light with lens. Apart from the hardware aspect, according to theexperiments, we can see that the different temperatures between at setting up themodels and at predicting the results will affect the prediction results in a way, and theaffection extent will be increased along with the increase of the temperaturedifference. Moreover, the light energy that can be detected will be different with thecontact pressure, and the match of the refractive indexes between the tissue and thesurroundings will enhance the light transmission depth, which will take moreinformation of the tissue. So, during the measurement, we should ensure the sametemperature between measuring and predicting, the same contact pressure at everymeasurement and the match of the indexes between the tissue and the surroundings aspossible as we can.

要提高测量精度，从硬件方面就必须有一个稳定的、信噪比较高的分光光路系统，以此得到稳定的近红外光来进行无创检测，针对这个目的，我们设计了 AOTF 分光光路系统，并在系统中采用一定的措施如三层灯室结构、遮光筒以减小杂散光、起偏和检偏来减小 0 级光影响、透镜汇聚等等来提高出射光的能量和系统的信噪比；从其他影响因素方面，通过实验，我们得到不同的建模温度对于预测结果有一定的影响，且影响随着温度的差值越大而越大，另外散反射光谱的能量也随着接触压力的不同而不同，组织的折射率与环境匹配将加大光的入射深度，从而能携带更多的组织信息，所以在测量时我们尽可能保证同一温度、保证同一接触压力以及利用物理或者化学的方法使组织与环境折射率匹配等。

Methods Realtime fluorescence quantitive RTPCR was used to detect the expression level of Tiam1 mRNA in gastric cancer tissues of 66 cases, paracancerous tissues (over 3cm) of 66 cases and gastric benign lesion tissues of 11 cases. Meanwhile, CK18 was selected as the hallmarker for lymph node micrometastasis to detect the level of CK18 mRNA expression in 199 lymph nodes from 24 cases of gastric carcinomas diagnosed negative by pathologic examinations.

采用TaqMan探针实时荧光定量PCR方法，定量检测66例胃癌患者癌组织、癌旁组织及11例胃良性病变组织中的Tiam1 mRNA拷贝数，同时将CK18作为淋巴结微转移的标志基因，定量检测24例病理诊断无淋巴结转移的胃癌患者199枚淋巴结的CK18mRNA拷贝数。

Methods The expressions of PCNA were checked by means of immunohistochemistry in the tissue of hydatidiform mole of 32 cases.

葡萄胎组织32例，应用免疫组织化学方法染色检测葡萄胎组织的PCNA表达。

On the Mechanism of Populating d~3∏ State of NaK by LIF Method;2. We apply the technology of LIFto diagnose hepatic fibrosis which is induced by different matter.

本文应用激光诱导荧光的方法，对不同物质诱发白鼠的肝纤维化的肝组织进行了荧光光谱分析，发现经过注射不同物质的白鼠的肝组织与正常白鼠的肝组织的荧光光谱有明显不同。

Methods: The immunohistochemistry was used to investigate the protein expression of p27 in 46 cases of osteosarcoma and 16 cases of osteochondroma. The expression of p27 and histological category and differentiation levels of osteosarcoma were observed.

应用S-P免疫组织化学方法检测46例骨肉瘤和16例骨软骨瘤组织中p27的表达，并观察p27表达在骨肉瘤组织学分型、分化程度等临床指标之间的差异。

Objective To research effect on tendon healing and adhearing after tendontransplanted with vascular vessel and protect paratenonMethods 30 rabbits were regarded as study objects ,the double Achillestendons were cut off and anastomosd in 24 ,the left Achilles were anastomosedwith protecting paraneton and circulation under microscope as theexperimental group; the right Achilles were handled as the left exceptresecting the peritendineum as the control group; the another 6 as thenormal group without any surgical intervene. All the animals wereimmobilized with plaster 3 weeks.

目的 研究吻合血管、保留腱膜肌腱移植对肌腱愈合及粘连影响方法以30只健康中国白毛红眼兔为实验动物，双后肢自身对照，实验组取左后肢于镜下保护好腱周血供，在腱周组织表面用尖刀片轻柔作一约0.5cm切口，挑出一束跟腱刀片横断，3-0无创缝线改良Kessler法缝合，再间断缝合腱周组织；对照组取右后肢，游离并切除腱周组织，余操作同实验组；空白组：不予任何外科干预。

Roentgenographic and histologic observation confirmed that bBMP had efficient bone morphogenetic activity.

论文第一部分参照杨连甲的方法从小牛骨基质中提取bBMP，经SDS-PAGE电泳分析，bBMP保留有BMP活性蛋白成分，植入小鼠股部肌肉后的X线及组织学观察发现，所提取的bBMP具有较高的骨诱导活性，诱导成骨的时间和过程与以往的报道一致。bBMP诱导骨形成过程呈现连续又具显著的阶段性，本研究结合组织学和组织化学观察的特点，将其全过程分为四个时期。

Methods: Immunohistochemistry was applied to examine the expression of PTEN, P27 and cyclin D1 in 58 cases of human colorectal carcinoma, 58 cases of tumor sideward mucosa and 14 cases of normal colorectal mucosa.

应用免疫组织化学方法检测58例大肠癌组织、58例癌旁组织和14例正常大肠黏膜中PTEN、 p27和cyclin D1蛋白的表达情况。

Positive expressive rate of cyclin D1 in colorectal carcinoma was 60.34%（35/58）, which was higher than that in tumor sideward mucosa and normal colorectal mucosa.

应用免疫组织化学方法检测58例大肠癌组织、58例癌旁组织和14例正常大肠黏膜中PTEN、 p27和cyclin D1蛋白的表达情况。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。