组织方法

decrease or absence expression of pten and fhit protein play an important role in the genesis and development of gastric carcinoma; the two proteins can be used to judge carcinomatous biological behaviors and prognosis.

本研究采用免疫组织化学方法检测56例胃腺癌组织及10例正常组织中pten和fhit基因蛋白的表达情况，探讨其在胃腺癌发生、发展中的作用。

With research results from foreign scholars', a questionnaire of entrepreneurial organizational learning assessment has been established. Based on the 193 qualified samples from more than 53 entrepreneurial enterprises, confirmative factor analysis was conducted.

第一个子研究结合了March（1991）组织学习理论中探索式学习和开发式学习概念界定和分类方法，以及认知学习和行为学习的理论基础，提出一个整合的组织学习内容构思框架，将研究一得到的五种组织学习要素整合到这个构思框架中。

Methods①The animal model of braincontusion caused by free drop hammer was established.②The injuredtissue of rat brain were stained by TUNEL for apoptosis,immunohistochemistry for Caspase-3 and Feulgen"s for DNA. Image analysistechnique and the statistical method were employed to explorate thetemporal changes of injury time.③The DNA was extracted from ratcontusive tissue of brain and assayed by gel electrophoresis toinvestigate the relationship between the DNA fragmentation and injurytime.④One handred and seventeen cases of death from traumatic braininjury were retrospective researched to investigate the characteristicof TBI in forensic medicine.⑤The contusive tissue of human brain werestained by TUNEL, Caspase-3 immunohistochemistry and Feulgen"s methodsin the same way to analyze and disclose the linear relationship betweentemporal changes with injury time.

方法①建立大鼠自由落体打击脑损伤模型；②对不同损伤时间组的大鼠脑挫伤组织进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色，结合图像分析技术和统计学分析，探讨脑挫伤后神经细胞凋亡、DNA片段化和含量的时序性变化；③提取大鼠脑挫伤组织DNA进行琼脂糖凝胶电泳分析，观察DNA片段化随损伤时间的变化特点；④对117例颅脑损伤致死案例进行回顾性分析，探讨其法医学特点；⑤选择不同损伤时间的人脑挫伤组织同样进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色和分析，观察上述指标与损伤时间的关系。

Methods Immunohistochemical streptavidin-peroxidase method was used to detect the expression of MMP-2, MMP-9, TIMP-1, TIMP-2 in 20 cases of normal human cytotrophoblast cells,15 cases of partial hydatidiform moles, 55 cases of complete hydatidiform moles, 15 cases of invasive moles and 8 cases of choriocarcinoma.

应用链霉菌抗生物素蛋白-过氧化物酶免疫组织化学方法对20例正常早孕(2周)绒毛组织、15例部分性葡萄胎、55例完全性葡萄胎、15例侵蚀性葡萄胎、8例绒毛膜癌组织中MMP-2、MMP-9、TIMP-1、TIMP-2进行检测。

This paper presents a study of Eucalyptus dunnii tissue culture, using its seeds as explants. The results reveal that MS medium is a suitable basic medium for its seeds to germinate and grow; that MS+KT1.0 mg/L+2, 4-D2.0 mg/L+Homocysteine30mg/L is a good medium to induce its seeds to dedifferentiate directly into callus; that H+6-BA2.0 mg/L+IAA0.2 mg/L is a good medium to make its bud on the seedling reproduce more buds; that MS+6-BA2.0 mg/L+NAA0.2 mg/L is a better medium to induce its root on the seedling into callus; that B5+6-BA2.0 mg/L+2, 4-D0.2 mg/L can induce its under-hypocotyl to differentiate into buds; and that B5+Ad2.0 mg/L+IAA0.2 mg/L can induce its under-hypocotyl into callus to generate normal buds.

以邓恩桉种子为外植体，探讨用最少种子快速繁殖最多幼苗的方法，结果表明：MS培养基是较适合邓恩桉种子萌芽和生长的基本培养基；诱导种子直接脱分化成愈伤组织的较佳培养基配方为MS+KT1.0 mg/L+2，4-D2.0 mg/L+半胱氨酸30 mg/L；以邓恩桉实生苗的芽来繁芽的较理想培养基配方为H+BA2.0 mg/L+IAA0.2 mg/L；邓恩桉实生苗的根诱导愈伤组织的较佳培养基配方为MS+6-BA2.0 mg/L+NAA0.2 mg/L；诱导邓恩桉下胚轴分化芽较佳培养基配方为B5+6-BA2.0 mg/L+2，4-D0.2 mg/L；诱导邓恩桉下胚轴脱分化为胚性愈伤组织的较佳培养基配方为B5+Ad2.0 mg/L+IAA0.2 mg/L。

There are several decision-making problems in the design of strategetic performance management system. A manufacturing company is given in this article to illustrate the way, we use Nash Equilibrium to establish a supervise model to determine system structure, we use AHP to determine the key business process of the company. Also it gives the brief clarification to classify and delaminate the KPIs.

战略性绩效管理系统的设计中存在很多决策问题，本文以一家制造业公司为例，介绍了应用Nash均衡建立绩效监察博弈模型，确定组织的绩效系统规模；通过AHP方法的创新应用，确定组织的关键业务领域，同时对组织关键业绩指标分类分层设计的思路做了简要阐述。

Methods: A sepsis model of mice made with cecum deligation and perforation was established,and a radioimmunoassay for FABP and 96-well spectrophotometry assays for myeloperoxidase and superoxide dismutasewhich were related with clearance of free radicals,were used to detect their levels in lung and intestine ho...

建立小鼠盲肠结扎致脓毒症模型，采用放射免疫分析法和96孔分光光度分析法分别检测肺肠组织匀浆液中FABP及髓过氧化物酶、超氧化物歧化酶等与自由基清除相关的酶的水平、同时以HE染色方法观察肺肠组织病理组织学改变。

Methods HA and TGFβ1 were measured concurrently in synovial fluid samples from 23 patients with mild OA, 28 patients with severe OA and 6 patients with disciform meniscus. The determinations of positive rate of cellsin genual synovium with HA and TGFβ1 were detected by using immunohistochemical techonology.

提取23例OA病变轻微患者，28例病变严重患者，6例对照者的滑膜组织及关节液，采用免疫组织化学方法检测膝关节滑膜组织中HA、TGFβ1的阳性细胞率及通过夹心ELISA法测定膝关节滑液中HA、TGFβ1含量。

Methods IL-1and MMP-13were measured concurrently in synovial fluid samples from23patients with mild OA,28patients with severe OA and6patients with disciform meniscus.The determinations of positive rate of cells in genual synovium with IL-1and MMP-13were carried out using immunohistochemical techonology.

提取23例OA病变轻微患者、28例OA病变严重患者、6例对照者的滑膜组织及关节液，采用免疫组织化学方法检测膝关节滑膜组织中IL-1、MMP-13的阳性细胞率及通过夹心ELISA法测定膝关节滑液中IL-1、MMP-13含量。

The first chapter introduces the background、significance、framework and methods. In the second chapter, it analyzes basic theories. The third chapter presents the imbalanced strategy of Tianjin Unicom in all-business operation. The fourth chapter introduces organizational structure reform. In the fifth chapter, it studies the lever which promotes structure reform. In The last chapter, the shortage and discussible questions are given.

本文共六章节，第一章介绍了论文的选题背景和意义、研究思路和研究框架、研究方法和研究内容；第二章阐述了本研究所用到的相关背景知识及理论；第三章以看不见的资产动力论为框架，论述了天津联通在全业务运营条件下的&不均衡&经营战略；第四章分析了与经营战略相适应的组织架构及组织形态，第五章深入研究了撬动天津联通组织变革的杠杆，第六章在总结前文基础上，提出了不足和有待讨论的问题。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。