组织方法

The result showed that high quality genomic DNA could be got from both fresh and frozen tissues except scale by common method. Tissue fixed with 70% alcohol and 10% formaldehyde could also extract high quality genomic DNA after effective pretreatment. Noteworthy high quality DNA could be got from scale with fresh and some other fixation methods.

结果表明，除鳞片外，新鲜组织、-20℃冰冻组织以常规方法可提取出较高质量的DNA；保存于70%酒精或10%甲醛溶液中的组织，经预处理，亦可得高质量DNA；新鲜、冰冻、70%酒精或短时间10%甲醛固定的鳞片经适当预处理也可提取出较高质量的DNA。

Histone deacetylase 6(HDAC6) is microtubule and HSP90 associated protein, which control genetic transcription and change cellule motor ability by deacetylased microtubule and HSP90,and then participate tumorigenesis, growth, metabasis, and the production of tumor vessel.

研究方法： 1。应用激光捕获显微切割术联合逆转录-聚合酶链反应技术，检测36例行根治性切除的原发性肝细胞癌中心处非坏死癌组织及24例正常肝组织的HDAC6 mRNA在组织中的表达，并对HDAC6与肝癌发生发展过程中相关的临床病理因素间相关性进行研究。

Methods 210 cases of MB and 9 cases of small cell tumor of cerebrum were observed with histologic and immunohistochemical techniques.

采用组织病理学和免疫组织化学方法对210例MB和9例大脑原发性小细胞肿瘤的组织形态特征和标志物表达进行比较研究。

The deduced amino acid sequence of red sea bream MHC classⅡB 1(Chma-DAB*0101) had 87.1%,85.1%,87.1%,90.4%,87.1%,90.8% identity with those of red sea bream classⅡB 2, 3, 4, 5, 6, 7(Chma-DAB*0201-Chma-DAB*0701), respectively, and had 75.2%, 74.5%, 55.9%, 55.1%, 34.3% and 30.4 % identity with those of striped sea bass, cichlid, rainbow trout, Atlantic salmon, mouse and human, respectively.

以正常鱼和鳗弧菌感染后真鲷组织为材料，以反转录合成的第一链cDNA为模板，应用RT-PCR半定量检测方法对真鲷的MHCⅡB基因组织表达特点进行了研究，结果表明，在正常真鲷组织中，其中较强的表达于鳃、脾、头肾、肾、胃、小肠和心脏中，在肌肉和血中表达最弱。

Methods: 1. Experimental laparoscopic liver resection: We selected pigs as experimental models aiming to approach human beings physiological and anatomical condition. After CO2 pneumoperitoneum was established, the ligaments around liver were dissociated. Livers were cut off by the methods such as clamp dissection, microwave tissue coagulator, ultrasound dissector, Ligsure dissection, hand-assisted, short abdominal incision laparoscopy-assisted, and Endo-GIA switcher. The operation time and bleeding volume were compared between different methods of laparoscopic liver resection. The advantages and limitations of different methods were summarized. 2. Clinical laparoscopic liver resection: 15 cases with tumors in segment V of anterior right lobe or segments II, III of left lateral lobe or segment IV of left internal lobe were selected in this study. The liver diseases included hepatocellular cancer, hepatic cavernous hemangioma and hepatic focal nodular hyperplasia. The ligaments around liver were dissociated after CO2 pneumoperitoneum established. Laparoscopic liver resection were carried out by some of the methods combined such as clamp dissection, microwave tissue coagulator, hand-assisted, short abdominal incision laparoscopy-assisted, ultrasound dissector, rotation and suction dissector.

1、实验性腹腔镜肝切除：以猪为实验动物，旨在接近人体的生理、解剖环境下进行实验，建立操作空间后游离肝脏周围韧带，采用钳夹分离切肝法、微波刀切肝法、超声刀切肝法、Ligsure切肝法、手辅助腹腔镜肝切除法、小切口腹腔镜辅助切肝法、Endo-GIA切肝法等方法实施腹腔镜下肝切除，比较不同切肝方法的手术时间、出血量，总结各种方法的优越性及其局限性。2、临床腹腔镜肝切除：选择肿瘤位于肝右前叶第Ⅴ段、肝左外叶第Ⅱ、Ⅲ段及左内叶第Ⅳ段的15例患者，病种包括原发性肝癌、肝海绵状血管瘤、肝脏局灶性增生，建立气腹后游离肝脏周围韧带，采用钳夹分离断肝、微波刀固化后断肝、手辅助腹腔镜肝切除、小切口腹腔镜辅助肝切除、超声刀断肝、旋吸断肝等多种方法分离切线肝组织，暴露管道结构，再采用高频电凝、钛夹夹闭、缝扎止血、止血纱布覆盖、氩气刀止血、生物胶止血、Endo-GIA处理等多种方法相结合处理肝断面，统计手术完成情况、手术时间、手术出血量、术后并发症及术后住院时间等，评价腹腔镜肝切除的可行性及安全性。

Abstract］Managers of NPOs have early began using accountant system, internal affairs management, human resource management and strategy planning as a set of operation and controlment method. However, marketing is the latest function to take into account.

非营利组织的管理者很早就开始了会计制度、内务管理、人事管理、战略计划等在营利组织中广泛使用的管理和控制方法，市场营销是最后一个被非营利组织所采纳的职能。

In the present experiment, the tender stems of Dracaena cambodiana were used as explants to establish a two-step tissue culture and regeneration system. The effects of types and concentrations of plant hormones on the induction of callus were studied. Based on these, the reactions of callus regeneration to different mutation-induced approaches (60Co irradiation, EMS and colchicines), the regeneration ability of the treated callus as well as the characters diversities (including the appearance traits and cytological observations) of the regeneration plants were investigated.

本试验以龙血树幼嫩茎段为材料，通过对龙血树幼嫩茎段两步培养法再生的研究，建立了龙血树幼嫩茎段离体再生体系，证明了愈伤组织的发生和激素种类与浓度的关系；并在此基础上研究了愈伤组织再生对不同人工诱变方法（60Co辐射、EMS、秋水仙素）的反应、被处理愈伤组织再生能力和再生植株性状多样性、以及再生植株不同生理角度的变异性，得到了2株变异株系。

Thus this study focus on the the effects of mechanical forces and 1,25- 2D3 on the expression of ODF and OCIF by periodontal tissue in vivo and periodontal ligament cell and osteoclast in vitro,thus clarify the molecular biology mechenism of tissue remodeling,and explore a new way in orthodontic clinic to accelerate the dental movement speed by adding some ectogenesis stimulating factors.

研究内容如下：一牵张力作用下牙周组织ODF及OCIF表达变化的研究目的：运用原位杂交的方法，观察ODF及OCIF在正畸大鼠牙周组织改建过程中张力侧的表达变化，探讨ODF及OCIF与正畸牙周组织改建的关系。

Results: miR27a was identified as the most stable reference gene by geNorm and Normfinder software. Among the 52 microRNAs detected by real time PCR array, miR-33 and miR-19a were significantly down-regulated, whereas miR-130 were significantly up-regulated in the hippocampus of Ts65 Dn mice as compared with the euploid control mice. The expression of miR-802, which was the trisomy chromosome 16 derived microRNAs, was very low in hippocampus with no difference between the two groups.

结果：geNorm和Normfinder两种方法均选定miR-27a为最稳定的内参照基因，共检测了52种microRNAs，其中miR-33和miR-19a在Ts65Dn小鼠海马组织中表达显著下调，miR-130在Ts65Dn小鼠海马组织中表达显著上调，小鼠16号染色体三体区段包含的miR-802在Ts65Dn小鼠和对照二倍体小鼠海马组织中表达量均低，差异没有统计学意义。

Find short time face guard suck high-density method of oxygen raise the oxygen content while organizing.

在PDT治疗中首次提出了以氧分压来间接反映组织氧含量的观点，通过定量测定静脉血的氧分压间接反映组织氧含量的变化规律和趋势，并提示运用短时间内面罩吸入高浓度氧的方法来提高组织中的含氧量。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。