组织方法

A systematic study was made on the induction of callus from terminal bud, cotyledon, plumular axis and the regenerated plantlets from terminal bud in Camptotheca acuminata Decne, and a large number of calli and cluster buds were obtained.

系统研究了以喜树顶芽、子叶、胚轴为外植体诱导愈伤组织的培养方法和以顶芽为外植体快速繁殖喜树的方法，获得了大量的愈伤组织和丛生芽。

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

Of the index finger back side island flap, the seaming finger nerve back lateral finger artery reversiveisland flap, the neighbour finger skin flap, the dorsal metacarpal artery reversive island flap and theposterior interosseous artery reversive forearm flap, groin skin flap,ilium skin flap and thoracic -umbilical flap were 197 cases.

杜昭，蔡文，黄德征（广西医科大学第六附属医院手外科，广西玉林537000）摘要：目的探讨手部创伤组织缺损显微外科修复方法及其效果。方法采用食指背侧岛状皮瓣、缝接指神经的背侧支指动脉逆行岛状皮瓣、邻指皮瓣、掌背动脉逆行岛状皮瓣和前臂骨间后动脉逆行皮瓣、髂腹股沟皮瓣、髂骨皮瓣及胸脐皮瓣转位修复手部创伤组织缺损197 例。

Methods:Thirty six mice were inoculated with the sarcocarcinoma 180 according to the standard method,after 10 days,weighed the tumor and body.The expressions of bcl2 and p53 proteins in the tissue were studied by immunohistochemistry; and the activities of SOD, GSHPx and the content of MDA were detected by colorimetric method in serum.

按照标准方法给36只小鼠接种S180肉瘤后，给予珍珠梅提取物10 d，观察瘤重及体重，免疫组织化学方法检测肿瘤组织中的bcl2和p53蛋白，比色分析法检测血清SOD，GSHPx的活性及MDA含量的变化。

Methods:Thirty six mice were inoculated with the sarcocarcinoma 180 according to the standard method,after 10 days,weighed the tumor and body.The expressions of bcl 2 and p53 proteins in the tissue were studied by immunohistochemistry; and the activities of SOD, GSH Px and the content of MDA were detected by colorimetric method in serum.

按照标准方法给36只小鼠接种S180肉瘤后，给予珍珠梅提取物10 d，观察瘤重及体重，免疫组织化学方法检测肿瘤组织中的bcl 2和p53蛋白，比色分析法检测血清SOD，GSH Px的活性及MDA含量的变化。

The paper sees Horton code as a primary characteristic value and generalizing condition of the river network, and selects, simplifies and generalizes river based on scale of before and after generalization,, The main contents of paper include:(1)Reviews generalization concept, arithmetic operators, methods, influence factor and frame of map generalization and design, analyze core theory of feature —oriented map generalization, discuss the main context and applicable method of river feature generalization(2) Summarizes natural form and spatial configuration characteristic of the river network, and describe the river network using digraph Paper mostly researches tree —like river, identifies the direction of river based on the angle of the main and secondary reach, and identifies the main river based on most length and predominant continuation of the flow direction along the major channel , then receives the grade of river by recursion and structuralize river network, builds hiberarchy rank system of river based on Horton coding(3)Designs the model of river network based on Horton code, and organizes the data of node, branch and river.

本论文把Horton编码作为水系网的一个主要特征值并以此作为综合条件，并根据综合前后的比例尺对水系网进行选取、化简和概括。论文的主要工作包括：(1)总结地图综合的概念、算子分解、实现方法、主要影响因素和总体设计框架，分析面向地理特征的制图综合的核心理论支撑，在此基础上讨论水系专题要素综合的主要内容和综合适用的方法。(2)总结水系网的自然形态和空间结构特征，用图论对水系网进行描述。以树状水系作为主要研究对象，依据树状水系的主支流交汇关系确定水系流向，依据水系中主流的长度最长和主流保持原有的流向趋势确定主流，按此递归得到河流的不同级别，对基于河段组织的水系网结构化，自动建立水系的Horton编码。(3)设计基于Horton码的水系网模型，对结点、河段、河流和河系的数据进行有效的组织。

A simplified model was proposed to simulate material microstructure according that metal solidification is a Monte Carlo process. First, use the point of"putting randomly, growing uniformly"to generate grain boundary, then recognize grain boundary trifurcate points, eliminate bad trifurcate points, and distinguish the connections among these points, thus obtain straight grain boundary picture.

根据金属凝固过程是—Monte Carlo过程，建立了金属材料显微组织的简化Monte Carlo方法，采用&随机投放，等速生长&的方法形成矩齿形晶界图，并通过逐点判断法识别出晶界三重点，剔除奇异三重点，并识别出晶界及晶界间相邻关系，从而获得晶界结构的直线近似形态图，为细观组织短裂纹的数值模拟作好准备。

The extopic expression rate in metastatic carcinoma was significantly higher than that in primary lesion(45.68% vs. 28.40%,p.01),especially in tubiform adenocarcinoma.

方法］ 肠癌症状组织芯片技术，应用免疫组化方法检测81例结肠癌原发灶及其淋巴结转移灶组织中e-cad和β-cat的表达。

Several regenerative procedures have been used for intra-bony defects including guided tissue regeneration, autografts, allografts, alloplasts, and combination of these techniques. These procedures have been reported to show different degrees of success.

目前常用的再生治疗方法有植骨术、引导性组织再生术（guided tissue regeneration，GTR）或二者的联合应用等，上述方法可以获得不同程度的成功率，但组织再生程度有限。

The differences of the micro structure and properities of the bulk sinters, between the fabrication methed of powder metallurgy and reactive synthesis for silver tin oxide composite, are studied in prensent thesis. With the increase of the tin oxide content in silver matix, the electric conductivity of the composite tended to decreace. While in the same tin oxide content, the electric conductivty of the composite fabricated by reactive synthesisis better than that by powder metallurgy. One of the reasons is that the regular net structure existing in former composite.

研究了两种方法即粉末冶金法和反应合成法制备的银氧化锡复合材料烧结坯在组织上的区别和性能上的差异，两种方法制备的银氧化锡复合材料，随着氧化锡含量的升高，材料导电性均呈下降趋势，而相同成分的材料，反应合成法制备的银氧化锡复合材料的烧结坯的导电性要优于粉末冶金法，有规则的网络状组织结构是反应合成法制备的烧结坯导电性好的原因之一。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。