组织方法

In chapter Five, A simulation method for ultrasound backscattered signal is proposed, which take involves ultrasound propagation in soft tissues and its interaction with tissues and different kinds of scattering patterns in tissues. A new scatterer spacing imaging method based on wavelet transform theory has been developed.

第五章提出了基于散射元空间分布的生物软组织的超声背向散射信号建模与仿真的新方法，这种方法的特点是综合考虑了散射元空间概率分布与组织中的超声衰减现象这两种不同类型的效应，同时还考虑了肝脏组织不同类型的散射模式。

Objective To reconstruct new corneal stroma in order to provide a basis for future studies on the reconstruction of cornea.

目的 探索组织工程技术构建角膜的可行性，并为其提供构建角膜的理论依据和参数。方法应用组织工程方法构建角膜基质层。

And then, this dissertation discusses the method of determing the organizational culture consensus unit and the aggregation operator based on the consensus unit.

随后，研究组织文化一致性单元确定方法和组织文化维度集结计量方法。

Objective To study the expression and significance of p53protein In gastric carcinoma and precanˉcerous lesions.Methods The expressions of p53protein were detected by immunohitochemical method in74chronic gasˉtritis,59intestinal metaplasia,66dysplasia and81gastric carcinomas.

目的 探讨胃癌及癌病变组织中p53基因蛋白的表达及意义方法应用免疫组织化学染色方法对74例慢性胃炎，59例肠上皮化生，66例异型增生和81例胃癌进行了p53基因蛋白检测。

BACKGROUND: Many studies have achieved certain progress in repair of the osteochondral defect by tissue-engineered chondral composite graft which was constructed with different methods. But the existing problems of these methods are presented that the coupling of interfaces of bone and cartilage in graft and host is not ideal.

背景：国内外许多研究通过不同的构建方法构建组织工程化软骨支架复合体修复骨软骨联合缺损，且取得了一定的进展，但目前各种方法存在的问题突出表现为组织工程化的软骨和骨组织之间的界面、移植体和宿主骨和软骨之间的界面耦合不够理想。

Extraction of high-quality RNA from Panax ginseng tissues is particularly difficult due to high levels of polyphenolics and polysaccharides, or other compounds that bind and/or coprecipitate with RNA. The methods such as guanidinum thiocyanate, CTAB and modified Trizol were applied to isolate high purity and integrity RNA from Panax ginseng.

针对人参组织多酚、多糖类物质含量较高的特点，比较了改良的异硫氰酸胍法、改良的CTAB法和改良的Trizol法等3种不同的RNA提取方法。3种改良的方法均能从人参组织中提取到总RNA。

After varied survival periods, the model mice were sacrificed to get the specimens of SN fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry to label the DA neurons and Calbindin D28k-containing neurons with the antibodies against tyrosine hydroxylase and CR respectively. The labeled cells were counted under microscope and analyzed statistically.

模型动物存活1周后，一部分取其中脑黑质节段，固定、包埋做连续冠状石蜡切片，以免疫组织化学染色方法，显示各组酪氨酸经化酶和钙结合蛋白表达阳性细胞，光镜观察并细胞计数，统计学分析；另一部分取其中脑黑质组织匀浆，行TH、CB的Westem blot免疫印迹方法检测，用LabWorks软件分析处理。

Methods Semiquantitative RT - PCR was used to detect the expression differentiation of Canstatin among NPC tissues, NPC cells and normal nasopharyn- geal epithelium.

方法利用半定量RT-PCR方法检测Canstatin在鼻咽癌组织、鼻咽癌细胞和正常鼻咽组织中的表达，比较它们之间的表达差异。

The expression of VEGFmRNA was valued by in situ hybridation method and the expression of Fit-1 and MV count was investigated by using immunohistochemical method.

应用原位分子杂交方法研究VEGFmRNA的表达，应用免疫组化方法研究Flt-1表达及MV的计数。结果：增生性瘢痕和瘢痕疙瘩组织中MV数目多於正常皮肤和扁平瘢痕组织中的MV数目。

Methods vWF protein was examined using immunohis-tochemical SABC staining method in 54 cases of osteosarcoma and 10 cases of osteochondroma.

方法应用免疫组织化学SABC方法检测10例骨软骨瘤和54例骨肉瘤组织中的vWF表达情况。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。