组织方法

Methods The immunohistochemical method and Western blot method were used to determine expression of COX-2 protein in specimens of 47 cases (33 cases by IHC and 14 by Western Blot ) of nasopharyngeal carcinoma and 24 cases of normal nasopharyngitis tissues.

分别应用免疫组化方法和蛋白印迹方法检测47例鼻咽癌组织（其中用IHC33例，Western Blot14例）及24例鼻咽部炎症组织中COX-2蛋白的表达。

Objective To study the expression of CD44v6,COX-2 and MMP-2 in gastric carcinomas and there relationship to lymph node metastasis.Methods The expression of CD44v6,COX-2 and MMP-2 in gastric carcinomas were detected by immunohistochemical method,and their relationship with lymph node metastasis was analyzed.

目的 研究胃癌组织中CD44v6、COX-2、MMP-2的表达及其与胃癌淋巴结转移的关系方法用免疫组化方法检测胃癌组织中CD44v6、COX-2、MMP-2的表达情况，并分析其表达与胃癌淋巴结转移的关系。

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分，(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况；(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法，分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况；(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价；(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用；(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

We study the expression levels of GLUT1 in 16 samples of esophageal cancer and their normal mucosa by reverse transcriptasepolymerase chain reaction and immunohistochemical staining, analyse the correlation between the expression level of GLUT1 mRNA and protein in the tumor cells. Then we study the expression levels of GLUT1 in 55 patients with esophageal cancer by immnohistochemical staining, analyse the relationship between GLUT1 expression and clinical features of the patients.

我们采用逆转录聚合酶链反应和免疫组化染色的方法，研究GLUT1 mRNA及蛋白在16例食管癌病人的肿瘤组织和相应正常食管粘膜的表达水平，同时分析食管癌组织中GLUT1在mRNA水平和蛋白水平表达的相关性；采用免疫组化染色的方法研究55例食管癌病人肿瘤标本的GLUT1表达水平，并分析其与肿瘤的临床特性及预后的关系。

Methods SP immunohistochemical staining was used to detect the level of VEGF and EG-VEGF in 85 cases of the epithelial ovarian tumor tissue(25 cases of benign tumor,27 cases of ovarian carcinoma of stage FIGOⅠ、Ⅱ,33 cases of ovarian carcinoma of stage FIGOⅢ、Ⅳ)and 20 cases of normal ovarian tissue.The integral optical density was measured.

方法应用免疫组织化学SP法和形态定量学方法检测85例卵巢上皮性肿瘤(良性25例，FIGOⅠ、Ⅱ期卵巢癌27例，FIGOⅢ、Ⅳ期卵巢癌33例)和20例正常卵巢(除外卵巢疾病的绝经前患者)组织中VEGF、EG-VEGF表达的阳性率及积分光密度值。

Objective To introduce a procedure of nympholepsy.Methods With the central part of the nympha excised,the hem preserved,the superior and lower part of the nympha served as flap pedicles,a double pedi-cles arc-shaped flap was elevated.The incision was conglutinated by ZT glue after the muscular layer sutured.Re-sults 36cases（62sides）were cured by this method with satisfying results.

目的 介绍治疗小阴唇肥的手术方法方法采用弧形双蒂瓣法行小阴唇整形术，保留小阴唇边缘，以小阴唇上、下部蒂，去除小阴唇中部一全层组织，形成一弧形双蒂组织瓣，缝合肌层后用医用粘涂ZT胶粘合切口。

In order to improve present Monte Carlo methods, method changing the node to optimistical orientation corresponding to the system having the lowest energy was proposed, which ensures the grain boundary moving to its curvature center. The new method avoided re-nucleation, and increased simulation efficiency, and made the simulation process more approaching the physics foundation of grain growth. The results show that the grain growth exponent, n=0.478, is close to its theoretic value, and the topological structure accorded with the normal grain growth of the real material.

简要回顾了晶粒长大理论并分析了现有Monte Carlo模拟方法的不足，提出了一个"择优转换"方法，确保节点转换时晶界向其曲率中心移动，以致模拟过程更接近物理基础，避免了重新形核的发生，使模拟效率大大提高，模拟的晶粒长大指数达到0.478，已接近理论值，组织形貌和实际材料正常晶粒长大组织基本一致。

Methods Surgical specimens/cell suspensions wore transplanted into the various tissues of BALB/c node mice (subcutis, cellular orthotopic injection into renal capsule, perinephrium, surgical orthotopic implantation into renal capsule). Tumorigenicity and metastasis were subsequently cvaluated. Immunohistochemical analysis was employed to determine the expression of VFGF, bFGF, P16, Bcl-2 and C-met in the metastatic and non-metastatic RCC xenograft.

采用皮下移植法、细胞悬液原位注射法、肾周筋膜内法、组织块原位移植肾包膜下法对BALB/c裸小鼠接种RCC组织块，观察各种方法的成瘤及转移情况，并采用免疫组化方法初步对比分析了VEGF、bFGF、P16、Bcl-2、C-met在裸小鼠体内的转移性和非转移RCC的表达情况。

According to the prior research , a highly efficient ext raction method was modified1 High2concent ration and DNA 2f ree RNA could be attained by using CTAB/ Guanidine Thiocyanate ext racion buffer1The total RNA can reach 1.7～2.0 of the OD260/ OD280 ratio with the yield of more than 015mg per gram of f resh tissues1 The method is suitable for high2quailty total RNA ext raction in leaves , root s , corollas , seeds and developing fibers.

本研究在前人方法的基础上对快速提取棉花组织总RNA 的方法进行了探讨。利用改进的CTAB 异硫氰酸胍裂解缓冲液，获得的RNA完整性好，无 DNA 污染，含量高，每克鲜组织能提取015mg 总RNA ， OD260/ OD280比值均在117～210之间。

Most of these scatterer spacing imaging sense fine-scale spatial features of tissue microstructure. The experimental results show that the method is an effective one for ultrasonic imaging.

另外，本章还提出了基于小波变换理论的生物组织散射元间距成像的新方法，实验结果表明散射元间距成像显示了更为细致的组织微观结构的空间特性，它是一种有效的超声成像新方法。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。