组织方法

Methods: The expression of VEGF by immunohistochemical staining was examined in 61 cases of the esophageal carcinomas and 13 cases of normal esophageal mucosas. Microvessel density in the cases was determined by FactorⅧ immunohistochemical staining.

应用免疫组织化学方法检测61例食管癌组织及13例正常食管粘膜组织的VEGF表达，并对血管进行Ⅷ因子相关抗原的免疫组织化学染色，计数微血管密度。

The survived tissue of the receptor site have good blood supply and there is no effect on the functions of the donor site.

结果 采用上述二种方法进行吻合血管的单一组织移植20例，组合组织移植66例，参与移植组织全部成活且具有良好的血液循环，受区残存组织及肢端血供良好。

The results showed that the mammary tissue cultured in superfusion system could keep normal tissue activity and ultrastructure within 24-72 h in DMEM plus 10 % calf serum, while mammary tissue stationary culture could keep normal tissue activity and ultrastructure within

结果表明：以1 mL/h 的速度灌流培养的奶牛乳腺组织在DMEM+ 10 %小牛血清培养基中24~72 h 内保持良好的组织活性和超微结构，贴壁培养的奶牛乳腺组织在DMEM+ 10%小牛血清的培养基中48~108 h 内保持良好的组织活性和超微结构，两种培养方法各有优缺点。

In the part of theoretical analysis,we begin with a basic concept "organization"of modern organization management theory, and give an explanation on the route of growth, which is for corporation organization to transform from simplicity to complexity, from suitability to unsuitability, then to transformation, and then transform from low-efficiency organization to high-efficiency one.

在这一部分，我们着重对企业组织的效率、环境变化和企业组织结构设计的原则以及企业组织再造的方法进行了分析，以期为农业银行进行组织创新找到一个理论上的支持。

Methods S-P immunohistochemical staining was used to detect the expression of VEGF-C and its reˉceptor Flt-4in10neighboring noncancerous tissue and60NSCLC cases of non-small cell lung cancer and32lymph nodes cases with lymph nodes metastasis of non-small cell lung cancer.

目的 探讨血管内皮生长因子C及其受体Flt-4在人非小细胞肺癌组织中的表达及其与淋巴结转移等临床病理特征的关系方法采用免疫组化S-P法检测60例NSCLC原发灶组织、10例癌旁组织、32例伴有肺癌转移的淋巴结组织中VEGF-C、Flt-4的表达，并将结果与肺癌的临床病理特征进行分析。

Morphological and structural characteristics of kidneys in Felis bieti and Felis lynx were investigated using anatomical and histological methods and the relationship between function and this character were discussed.

荒漠猫和猞猁肾组织结构的比较研究摘要为了了解荒漠猫和猞猁肾脏组织形态学的生态适应性特征，为保护野生动物资源和饲养繁殖管理提供组织形态学资料，应用解剖学和组织学方法观察了荒漠猫和猞。。。

The results manifest that the concentration of glycocholic acid has satisfactory linear correlation whith peak area, regression equation: Y= 39579X + 57823,R2=0.9984;The lowest detected concentration is 0.02μg/mL with S/N=3 ; Average recovery rate is 98.49%; RSD为0.52%～2.00%in day, RSD为0.74%～4.98%between days;The average recovery rate is 99.35%, RSD is 2.24% in the first team, and it is 100.87%, RSD is 4.73% in the second team at the stability test. When the concentration of glycocholic acid was detected in the liver, kidney and brain tissue, corresponding tissue concentration was calculated by preparing the standard curve with the glycocholic acid standard preparation in vitro, but the result can only reflect the relative chang law of the concentration of glycocholic acid in tissue but not in real tissue. The result of tissue stability experiment is consistent with that of the serum experiment.

结果表明，在0.15625～10.0μg/ml范围内血清中甘氨胆酸浓度与峰面积呈良好的线性关系，回归方程Y= 39579X + 57823，R2=0.9984；以信噪比S/N=3为标准，最低检测浓度为0.02μg/ml；平均回收率为98.49%；日内RSD为0.52%～2.00%，日间RSD为0.74%～4.98%；稳定性试验第一组的平均含量为99.35%，RSD为2.24%，第二组的平均含量为100.87%，RSD为4.73%；测定了肝、肾、脑组织中甘氨胆酸含量，采用体外甘氨胆酸标准品制备标准曲线的方法计算了相应的组织浓度，其结果仅反映组织中甘氨胆酸浓度的相对变化规律，不能体现真正的组织浓度值，但对组织样品的稳定性进行了较细致的考察，其结果和血样结果基本一致。

The present study employed synchrotron-based infrared microspectroscopy to establish the correlation of kinetic physisorption between wax molecules and oral cavity cells/ tissues for diagnosing oral cavity cancer. The absorbance of v CH2 and v CH2 of waxed sample in the spectral range of 3000-2800 cm^(-1) enabled to be a signpost of wax remained onto the cells or tissues during the kinetic dewaxing procedures.

本研究以同步辐射红外显微光谱为基础的红外动力学方法，探讨有机蜡对人类口腔细胞组织的物理吸附能力与细胞组织癌化的关连性，藉由量测有机蜡残留在细胞组织上动力学时间作为判定细胞组织癌化的依据。

Methods The expression of caspase-3 and PCNA was detected by tissue microarray and immunohistochemical method in 95 hepatocellular carcinoma tissues,68 parahepatic cancer tissues and 19 normal liver tissues.

应用组织芯片技术和免疫组化方法检测95例肝细胞肝癌组织、68例癌旁组织和19例正常肝组织中Caspase-3和PCNA的表达。

By randomly single-pass sequencing from 5'-end of muscle cDNA library and liver cDNA library from Silkie Fowl, 16600 ESTs of muscle tissue which are more than 100bp and uncontamination and 17733 ESTs of liver tissue which are more than 100bp and uncontamination have been obtained.

利用大规模DNA序列测定方法，对丝毛乌骨鸡胸肌组织和肝脏组织的2个cDNA文库在5'端进行了大规模的单向单次的随机测序，得到了肌肉组织的16600条大于100bp高质量无污染的ESTs和肝脏组织的17733条大于100bp高质量无污染的ESTs。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。