组织方法

Objective: To explore the expression of fatty acid synthase and its significance in esophageal carcinoma.

目的：研究脂肪酸合成酶在食管癌中的表达及其临床意义。疗法：通过免疫组织化学的方法对45例食管癌标本，6例不典型增生的食管粘膜和12例正常食管粘膜中的脂肪酸合成酶的表达进行检测；并与临床病理指标进行比较。

Starts with the three parts of the relationship of the villages and towns government and the villager committees and the relationship of the two rural committees and the relationship of the two rural committees and villager, analyzes how the agriculture tax-and-fee reformation effects on the villagers" autonomy systematically, and think the agriculture tax-and-fee reformation will be advantageous to die down "theadministeration turn" of the village committees, meliorate the relationship of the two rural committees and the crowd, alleviate the two rural committees antinomy, promote organization construction of the two rural committees, but in the meantime also bring some series problems for the village autonomy such as public finance problem, tax fee tail owe problem etc., in the end put forward that we should push forward the villager" autonomy of the post-agriculture tax ages from the four parts of the Grass-roots Government, relationship between officials and the masses, relationship between the two rural committees and the village collective economy.

本文针对以上问题，运用理论研究与实证分析、静态分析与动态分析相结合等研究方法，从乡镇政府与村民委员会关系、村"两委"关系以及村"两委"与村民关系等三方面入手，系统地分析了农业税费改革对村民自治的影响，认为农业税费改革将有利于减弱村民委员会的"行政化"、理顺村"两委"与群众的关系、缓解村"两委"矛盾、促进村"两委"的组织建设，同时也为村民自治带来了一系列问题，如财政问题、税费尾欠问题等，最后提出应从基层政权改革、干群关系、两委关系以及农村集体经济等四个方面来推进后农业税时代的村民自治。

And it prevent and control completely occurence of crack in the ferroconcrete cast-in-Place floor from nine respects such as the raw materials production control, construction process, construction organization design and construction measures. The application shows that the methods and measures of controlling crack are significant in construction. It provides the basis for further controlling cracks of ferroconcrete cast-in-place floor.

针对混凝土现浇板易产生裂缝这一现象，本文从施工振捣、抹面压光等7个方面揭示了裂缝生成的原因，并提出了从原材料进场、施工工序、施工组织设计、施工措施等9个方面来全面预防和控制现浇板混凝土裂缝的产生，实际施工实践证明这些防控裂缝的方法措施，具有较强的施工指导意义，为进一步做好现浇混凝土楼板裂缝控制提供了借签依据。

The microstructure of ferromagnetics for Fe-Al alloys as well as the effects of alloy elements and heat treatment on the damping behavior and mechanical properties of Fe-Al based alloys have been investigated systematically by means of optical microscopy,scanning electron microscopy.

采用光学显微和扫描电子显微观察等方法，系统地研究了Fe-Al系铁磁性阻尼合金的微观组织，考察了合金元素、热处理工艺对合金阻尼性能和力学性能的影响，探讨了Fe-Al合金产生高阻尼特性的微观机制以及Fe-Al系高阻尼合金的同质和异质焊接性能。

Antibody of mouse against rat of glial fibrilla acidic protein used to labeling astrocytes of the brain and antibody of rabbit against rat of c-fos labeling c-fos protein in the brain. The second fluorescence antibodies were FITC and Cy3, respectively. Observations of the results and quantitative analysis were conducted with LSCM.

在本实验中，我们应用免疫组织化学的双标方法，用小鼠抗大鼠胶质原纤维酸性蛋白标记大鼠脑内的星形胶质细胞，用兔抗大鼠c-fos标记大鼠脑内的c-fos蛋白，分别应用FITC和Cy3标记的荧光二抗来显示，最后用激光扫描共聚焦显微镜进行观察并定量分析。

［Objective］To approach the clinical characteristic,diagnosis and method of treatment of malignant fibrous histiocytoma of bone.

［目的］探讨骨的恶性纤维组织细胞瘤的临床特点、诊断及治疗方法。

Fiducial analysis is a kind of organization study that can enhance competition ability and imitate method.

基准分析是一种能够提高竞争力的组织学习与模拟方法。

And now that the existence of a well organized Jap fifth column in this country had been revealed, all civilians will be wise if they, too, familiarise themselves with positive ways of distinguishing a potentially dangerous Jap from a friendly Chinese.

组织严密的小日本的第五纵队已经在这个国家磨刀嚯嚯，所有官兵必须学会用正确的方法区分友善的中国人和存在潜在威胁的小日本。

RT-PCR/Southern blot was used to detect the change of Brn-4 mRNA expression in hippocampus after fimbria fornix transection.

取各组大鼠的海马组织，提取总RNA，采用RT-PCR/Southern 杂交方法分析穹窿海马伞切割后海马内Brn-4 mRNA的表达变化。

Then hippocampi were isolated and total RNA was extracted. RT-PCR/Southern blot was used to detect the change of Brn-4 mRNA expression in hippocampus after fimbria fornix transection.

取各组大鼠的海马组织，提取总RNA，采用RT-PCR/Southern杂交方法分析穹窿海马伞切割后海马内Brn-4 mRNA的表达变化。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。