组织培养

Mature and immature embryos of maize inbred line 87-1 were used as explants and calli were induced and cultured on improved N6 medium. The method of enzyme-linked immunosorbant assay was employed to detect endogenous contents of abscisic acid, indoleacetic acid, gibberellins (GA3) and cytokinins of immature, mature embryos and their calli during culture process.

以玉米自交系87-1的幼胚和成熟胚为材料，采用酶联免疫法测定了不同培养时间诱导的幼胚及成熟胚愈伤组织内源脱落酸、吲哚乙酸、赤霉素(GA3)和细胞分裂素含量的动态变化。

The methods of enzyme-linked immunosorbant assay were employed to detect endogenous contents of abscisic acid, indoleacetic acid, gibberellins (GA3, GA4) and cytokinins of immature embryos, embryonic and non-embryonic calli during culture process.

酶联免疫法测定不同培养时间后未成熟胚，以及由此诱导的胚性和非胚性愈伤组织，内源脱落酸、吲哚乙酸、赤霉素(GA3、GA4)和细胞分裂素(DHZR、IPA、ZR)含量。

METHODS: Keloid tissues with prominences and redness were harvested. Subsequent to primary cultures, dermal fibroblasts at passages 3 to 4 were used in this study.

取瘢痕疙瘩边缘突起发红的部分，按组织块法培养原代成纤维细胞，取第三四代的细胞用于实验。

To study the different reactivities of PDL cells to EMP5 we established the PDL cells culture model by tissue explant in vitro.

PDL细胞的原代培养及鉴定为研究EMPs对人PDL细胞的作用，采用组织块法成功地建立了人PDL细胞的模型。

The new cohort of experts believed child-rearing, education and relationships needed to be reorganised in accordance with the latest finding of scientific research.

新一帮专家相信养育孩子、教育、关系培养需要根据科学研究的最新发现进行重新组织。

In order to confirm this suspect, we choosed a cultured rat granulation tissue fibroblasts model in vitro and applied RT-PCR and cell-ELISA technology to detect the changes of firbroblasts intrinsic EGF, bFGF, TGFβ-1 with their receptors gene expressions and protein synthesises after stimulated by SP. Our purpose was to explore the possible effects and patterns imposed by SP on fibroblasts intrinsic growth factors and their receptors expressions, which maybe offer theoretical basis for promoting wound healing via improving nervous functions and regulating neuropeptides secretions.

为进一步验证这一推测，同时排除在体多因素干扰，我们采用了一种大鼠肉芽组织成纤维细胞体外培养模型，采用RT-PCR与细胞ELISA技术，检测SP刺激成纤维细胞后，成纤维细胞内源性EGF、bFGF、TGFβ-1及其受体基因表达和蛋白合成的改变情况，探讨SP对成纤维细胞内源性生长因子及其受体表达的影响以及方式，以期为经由改善神经功能、调节神经肽分泌途径促进伤口愈合提供理论依据。

Two weeks after the operation all rats were sacrificed and the following items were tested:(1) the concentration of endotoxin in portal and systemic blood;(2) the bacteria of mesenteric lymph nodes and livers;(3) insulin-like growth factor-l mRNA expression of the distal ileal mucosa;(4) the morphological changes of the ileal mucosa under microscope and electron-microscope. Results The translocation of bacteria and endotoxin occurred and the pathological change was observed two weeks after BDL.

两周后测定3组大鼠门静脉和体静脉血液的内毒素水平；对肠系膜淋巴结和肝脏进行细菌培养；用RT-PCR测定肠粘膜胰岛素样生长因子-1(IGF-1)mRNA的表达水平；取末端回肠组织观察肠黏膜病理变化。

The present study is designed to seek the evidences of regeneration of auditory epithelia and acoustic nerve in the postnatal chick, By using the methods of tissue cultures, scanning electron microscopy, autoradiography and the cell image analysis technique, we observed the regeneration of auditory hair cell after intense sound exposure, and also neuron repair following a mechanical injury with either in vivo or in vitro.

本研究应用物理性噪声，建立了内耳听器-基底乳头声损伤的模型和初级听神经元纤维机械损伤的模型。在此基础上应用体外组织细胞培养、扫描电镜、放射自显影、光镜以及细胞图象分析等多种形态学方法，综合分析了听觉毛细胞和初级神经元轴突的再生反应。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。