组织培养

Beijing Fatty Chicken, Langshan Chicken and Small-tailed Han Sheep were selected as experimental material. Fibroblast library was constructed through tissue confluency culture and cell freeze technology. Growth dynamics, genetic stability and contamination of of cross breeds etc.were detected through growth curve, chromosome analysis, isoenzyme analysis etc. Cloning and sequence of gene GPAT of Beijing Fatty Chicken was analyzed by cloning technology and software related.

本论文以北京油鸡、狼山鸡、小尾寒羊为研究对象，采用组织块贴壁培养法和细胞冷冻技术构建成纤维细胞库，采用生长曲线、染色体分析、同工酶分析等方法对细胞生长动态、遗传稳定性、种间交叉污染等进行检测；采用基因克隆技术和相关分析软件对北京油鸡GPAT基因进行克隆和序列分析。

During the course of culture in vitro, the biological function of human nasal septal cartilage gradually degrade in accordance with increasing times of passage, chondrocytes after the third generation are not suitable for human cartilage tissue engineering seeds or studying cytobiology.

人鼻中隔软骨细胞在体外培养过程中，随传代次数的增加而功能活性逐渐降低，第3代以后的细胞不适于作为人软骨组织工程种子细胞和用于细胞生物学等方面研究

During the course of culture in vitro, the biological function of human nasal septal cartilage gradually degrade in accordance with increasing times of passage, chondrocytes after the third generation are not suitable for human cartilage tissue engineering seeds or studying cytobiology.

人鼻中隔软骨细胞在体外培养过程中，随传代次数的增加而功能活性逐渐降低，第3代以后的细胞不适于作为人软骨组织工程种子细胞和用于细胞生物学等方面研究关键词：葡萄糖氨基浆糖类；软骨细胞；细胞外基质；生物材料

In order to deal with the phenomena of the "deaf-mute English" and wasting time for low efficiency in English teaching,to embody the position of"student-subject"adequately,to cultivate the group-cooperation of students,to educate the students to be accustomed to taking an active part in self-study、self-restriction,self-management and to make students get ready for social profession and life-long study.

为了改变目前高职英语教学中存在的&聋哑英语&和费时低效现象，在英语教学中建设好学生的学习型组织，能够充分体现学生的主体地位，培养学生的团队合作精神，使学生养成自觉学习、自我约束、自我管理的行为习惯，努力提高英语应用能力，为学生走向社会职业和终身学习作好准备。

Firstly, morphology, lactate dehydrogenase pattern and random amplified polymorphic DNA fingerprinting of three continuous marine fish cell lines of FG, SPH and RSBF, derived from flounder gill, sea perch heart and red sea ...

本文首先从形态、蛋白质和DNA三个层次水平对牙鲆鳃细胞系FG、鲈鱼心脏细胞系 SPH和真鲷鳍细胞系RSBF的特性进行了分析，并分别与其所对应的原代培养组织作了比较。

During the school as a student officer and as a class cadre of me, and always put their own quality first, strict demands on themselves, develop their own ways to learn how to master the knowledge and ability, after three years of refinement with a certain amount of organizational capacity.

在学校期间，作为做为一名学生会干事及班干部的我，始终把自身素质放在第一位，严格要求自己，培养自己如何去学习、如何去掌握知识的能力，经过三年的的提炼具有了一定的组织能力。

As a public relations manager at school , during the practical experience, I find my organizational capacity and collaboration increased and I've developed a sense of more innovation and management capabilities.

在校期间当过班上的公关部经理，在此锻炼下，让我的组织能力以及协作能力有所提高并培养了我的创新意识和管理能力。

In order to develop and utilize these inherent genetic resources, in this research, we established an experimental system for leafbase-derived calli of Allium mongolicum Regel from the isolation of protoplasts, cultivation and plant regeneration by cell engineering techniques.

为开发利用其固有的遗传资源，本研究利用细胞工程技术建立了沙葱叶基愈伤组织原生质体的分离、培养和植株再生实验体系。

It was decided that technical and vocational education in rural areas should accommodate local conditions and be conducted on a short-term basis. Where conditions permitted, emphasis would be placed on organizing technical schools and short-term training classes. To alleviate the shortage of teachers, vocational and technical teachers' colleges were to be reformed and other colleges and universities were to be mobilized for assistance.

这次会议作出的决议包括：农村地区的职业技术教育主管部门，应根据当地的实际情况，制定和适应短期发展目标规划，发展和当地情况相适应的职业技术教育；情况允许的地方，政府主管部门应把重点放在开办技术学校和组织短期职业技术培训班上；为缓解职业技术教育师资缺乏的状况，职业技术教师高等专科学校应进行改革，其他专科学校和大学也应为此调动各种资源协助培养职业技术教师。

Method］Isolated bMSCs were cultured in vitro. rh Fibroblast Growth Factor 1(rhFGF-1)、rh Transforming Growth Factor-β1(rhTGF-β1)、rh Insulinlike Growth Factor-Ⅰ were utilizated. The proliferation of cells was detected by MTT assay, and the macroscopic histology , HE staining and immunohistochemical examinations were performed to seek the best cell factor. In vivo , to investigate the repair of the articular cartilage, bMSCs combined with fibrin glue and rhTGF-β1、rhIGF-I was compared with control group.

方法］rhFGF1、rhTGF-β1、rhIGF-I单独或联合应用对骨髓基质干细胞进行体外诱导培养，应用常规染色、MTT、免疫组织化学染色的方法筛选诱导骨髓基质干细胞成软骨细胞的最佳细胞因子，并将其与骨髓基质干细胞复合于纤维蛋白凝胶制成凝胶复合物，直接种植到兔膝关节实验性关节软骨缺损处，并与对照组相比较，观察软骨修复效果。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。