组织培养

On the other hand, the apoptotic effects of mtDNA depletion in vivo are different from those observed in cultured cells because of the supplementation of pyruvate and uridine in the medium.

另一方面，mtDNA的减除对活体细胞的影响与在细胞培养所观察到之凋亡行为应有所不同，因为在活体组织内细胞并无法获得大量的丙酮酸。

By using electroporation transfects,the TGF〓expression vector were introduced into HL-60 cells.Thecells were allowed to grow for 1 day before beingsubjected to selection for the ability to grow in mediumcontaining 600ug/ml geneticin.Stable cell clones resistantto G418 sulfate were obtained after 2-3 WK growth withdrug-containing medium.Southern blot hybridization wascarried out.RNA slot have higher expression of TGF〓mRNA in HL-60 transfected.All results followed suggestedthat TGF〓 have suppressed the maliganant phenotype of HL-60 cells.

利用电击法将〓表达载体导入HL-60细胞中，经G418筛选培养20天后再行Southern杂交检测外源〓整合情况，利用RNA狭线杂交法证明了在转录水平上表达有所提高，进一步进行了流式细胞仪测定DNA含量，使细胞在〓期产生生长阻滞作用，细胞生长速度，在软琼脂中成集落数、在组织中的浸润性及在裸鼠中的成瘤性均发生变化，我们的实验结果表明TGF〓对HL-60细胞具有抑制恶性表型的作用。

Methods TILs were separated from tissue samples which obtained from the surgically removed tumor of 67 patients with lung cancer by mechanic and enzyme digestion and discontinuous density Ficoll-paque gradient centrifugation ways.

目的 从肺癌患者手术切除的肿瘤组织中分离出肺癌浸润淋巴细胞，经rIL-2体外激活培养，研究TIL体外分离、增殖的条件和特点。

The main techniques of this system are as follows: Simulated digital map of field surveillance radar, aircraft objects display and graphic operations Simulated mathematical models of ground motions of the aircrafts Simulated realization of all kinds of airport control orders Processing of aircrafts'taxiways selection Tight coupling of airport and approach control Designs of training courses about airport control based on the standard of International Civil Aviation Organization This paper introduces the design and realization of simulation system of the field surveillance radar in detail, including five parts: The first part generally introduces Civil Aviation Air Traffic Control and simulated training of air control radar, and then analyzes the simulation requirements of the field surveillance radar, finally, presents works of the author in this simulation system items.

机场场面监视雷达仿真系统是快速高效并且低成本培养和提高塔台管制人员特别是管制学员的塔台管制技能的重要工具和设备，该系统具有以下技术要点：机场场面监视雷达仿真数字地图和航空器目标的显示以及图形操作航空器地面运动的仿真数学模型各种机场管制命令的仿真实现地面航空器滑行路线选择处理机场管制与进近管制的紧密结合按照国际民航组织标准进行设计机场管制训练课程本文详细地介绍了场面监视雷达仿真系统的设计和实现，一共分五个部分：第一部分概括介绍民航空中交通管制和空管雷达仿真训练，分析机场场面监视雷达仿真的需求情况以及作者在该仿真系统项目中所做的工作。

Operations Assistant Beijing Evening News Group Contributing writer · Front came to visit our school and learning of foreign friends, the exchange of culture and spoken language skills · Screening Contributor articles, design layout and organize periodic summary.

业务助理北京晚报集团特约撰稿人·接待前来我校参观和学习的外国友人，培养了交流及口语技巧·筛选投稿文章，设计版面及定期组织总结。

Longer pre-culture times for the spheroids resulted in slower fusion, less coherence and increased length of rod microtissues.

对球体更长的预培养时间导致了融合速度减慢，粘附减少以及柱状微组织长度的增加。

Normal human fibroblast spheroids were self-assembled and cultured for 1, 4, or 7 days, then combined in trough shaped recesses. Over a 24-h period the spheroids fused to become a rod shaped microtissue and the kinetics and extent of fusion could be quantified by assessing rod contraction.

正常的人成纤维细胞可自我聚集形成球体，并培养了1、4、7天，然后在槽状的凹处中进行结合。24小时时间段过后，球体融合成柱状的微组织，融合的动力学及程度可以通过评定这种小柱的收缩而进行定量分析。

By using only a tiny piece of plant tissue,they can grow enough seedings for a forest.

用一小块植物组织，在实验室里就能培养出可以供一片森林使用的幼苗。

Company sales of the full range of cytokines, antibodies and cytokines detection kit, neuroscience and signal transduction research reagents, complement the various species, flow cytometry antibodies, plant tissue culture medium, culture additives, industrial chemical and biological materials, laboratory Pipettes, laboratory supplies and other products.

公司销售品种齐全的细胞因子、细胞因子抗体和检测试剂盒，神经科学和信号传导研究试剂，各个物种的补体，流式细胞术抗体，植物组织培养基、培养添加剂，工业生化原料，实验室移液器、实验室耗材等产品。

PEP1SOD1 fusion protein can be transduced effectively into endothelial cells of human umbilical vein, which provides a basis for future study involving direct delivery of SOD1 protein into tissues for therapy.

PEP1SOD1融合蛋白能高效地转导入体外培养的人脐静脉内皮细胞，为SOD1进入组织细胞内发挥治疗作用提供了实验基础。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。