组织培养

Anatomy study on the protocorm-like bodies formation and development of Cymbidium hybridium was conducted.

以大花蕙兰类原球茎发生发育不同阶段的培养物为试材，采用石蜡切片法对其进行组织学研究。

Strong specific staining was detected in lympho cytes, monocytes and neutrophils at sites of pulp inflammation.

探讨凝血酶受体在人正常、炎症牙髓组织及体外培养的牙髓成纤维细胞中的表达及其意义。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记，对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml)，以不同扫描序列T1WI，T2WI，T2*WI(GRE进行MR成像，再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像，并测量信号强度，进行统计学分析。3缺血再灌注肾损伤模型建立和处理，然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只：注入标记细胞者10只，注入未标记细胞者6只)，两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照，然后对收集的信号强度进行统计学分析。

To test whether ethylene oxide retained in the dialyzers might be neurotoxic, tissue culture medium was incubated in the blood compartment of dialyzers.

如果组织培养基在透析器内孵育，然后将鼠的背根神经节（DRG培养物暴露于此培养基中，则DRG的特异性变化会发生。

Objective To provide cells resource for experimental study on Vascular tissue engineering.

第一部分目的为培养组织工程化血管的实验研究提供细胞来源。

The cultured cells can meet the experimental expectations on vascular tissue engineering.

所培养的细胞可提供作进一步深入的血管组织工程的研究。

Methods We established the model of human hair follicle in vitro in Williams E serum-free medium. We can continuously observe morphologic change of hair follicle under an invert microscope. The length of hair follicle was measured by eyepiece micrometer and DNA synthesis rate of human hair follicle in vitro by measuring the rates of incorporation of 3H-TdR.Histology form was observed by Cryo-section and transmission electron microscope.

在Williams E无血清培养基中，建立离体人头皮毛囊培养模型；通过倒置显微镜观察毛囊的形态变化；目镜测微器测量毛囊长度；3H-TdR 掺入检测毛囊DNA合成率；组织学检测用冰冻切片光镜观察和电镜切片透射电镜观察。

Three healthy flap-eared New Zealand rabbits aged 4 months were aspirated on iliac crest under anesthesia for 6 mL bone marrow from which BMSCs were separated, then sacrificed and excised the lumbar intervertebral disc from which the nucleus pulposus cells were separated.

目的：针对椎间盘退行性变的组织修复目前集中于细胞移植治疗，实验在体外联合培养条件下观察骨髓基质干细胞对髓核细胞增殖分化的影响。

Set up as a joint project between the UN and the Chinese government to train professionals for the United Nations, the programme turned out 98 interpreters (out of 217 graduates), many of whom are now working for the UN and other international organisations, some for the Chinese government, and a few as full-time freelances.

做为联合国和中国政府共同开设的为联合国培养专业人员的项目，该项目共产生了98个口译员（除217毕业生以外），其中许多人现在给联合国和其他国际组织工作，一些给中国政府，还有一些做为全职自由翻译人员。

The basic units should work purposefully from the students′ actual conditions.They should make early head-hunt and start the training early with active education and investigation of activists.They should make their best to ensure the new members′ qualification up the standard to give qualified succ...

党的基层组织要结合学生的实际，有针对性地开展好工作，早选苗，早培养，积极做好入党积极分子的教育、考察工作，坚持标准，保证质量，加大工作力度，使党的事业后继有人

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。