组织培养

Histochemical assays of GUS gene showed those resistant callus and roots from transgenic plants cultured under light were no blue-stained with X-gluc, but callus and roots after 24 h treatment of darknees and then incubated with X-gluc could present blue. Fluorimetric enzyme quantitative analyses indicated GUS expression in transgenic plants was organ specificity.

转基因抗性愈伤和转基因植株根的组织化学GUS活性检测表明，光照培养下的抗性愈伤和转基因植株根不能使X-gluc显色，而暗处理24 h后的抗性愈伤和定植后转基因植株的根能使X-gluc显色。

Bull;To train managers to become business coaches , help enterprises set up Learning Organization Corporate University

培养经理人成为企业教练，协助企业建立学习型组织

This study will contribute to a better understanding of mechanism of bone remodeling during tooth movement which may set the basis for clinical work. Methods1. Observe the expression of ATF4 protein in periodontal tissues after orthodontictooth movementA titanium-nickel closed-coil spring was applied to the occlusal surface of the rat maxillary first molar (M1) with a hook and the upper incisors with a ligature wire.

但正畸骨改建与骨发育是两种不同的生理过程，ATF4在正畸牙齿移动牙周组织的表达和分布，在机械力的作用下体外培养的人牙周膜细胞中表达变化的情况，以及在牙周膜细胞向成骨样细胞分化的过程中所起的作用，国内外尚未有报道。

Constructivism advocates "a student-centeredlearning under the guidance of teachers". They agues that knowledge is not taught bythe teachers, but is acquired in a certain situation, that is, in the social and culturalbackground, through the Construction of Meanings by using the necessary materialsfor study, with the help by others (including teachers and learning partners). Theystress to create a variety of real language environments for the students, and pay muchattention to training the students actual ability of using the language. Its theory hasgreat inspiring and guiding significance in organizing teaching and compilingteaching materials.

建构主义提倡&教师指导下的，以学生为中心的学习&，认为知识不是通过教师传授得到的，而是学生在一定的情境即社会文化背景下，借助其他人的帮助，利用必要的学习资料，通过意义建构的方式获得的，强调为学生创设真实多样的语言环境，重视培养学实际运用语言的能力，其理论对组织教学、编写教材富有启发和指导意义。

In April, Atala and his colleagues rebuilt bladders for seven young patients using live tissue grown in the lab.

在4月份的时候，Atala和他的同事利用实验室培养出来的活组织为7 位年轻病人进行了膀胱再造。

In April, Atala and his colleagues rebuilt bladders for seen young patients using lie tissue grown in the lab.

在4月份的时候，Atala和他的同事利用实验室培养出来的活组织为7 位年轻病人进行了膀胱再造。

An in vitro endothelial and smooth muscle cell cocultured model, which simulates the cell arrangement of the wall of artery in vivo, was established.

作者建立一个新的摹拟动脉壁结构的内皮和平滑肌细胞体外联合培养模型，并以此检测内皮、平滑肌细胞在合成和分泌组织型纤溶酶原激活物中的相互影响。

Light microscope combined with stereological methods were used to observe and analyze renal tissue and corpuscles at different developmental stages in vivo and vitro.

结果 胚龄12天（E12）体外培养发育情况与在体无显著差异，其余各组随胚龄的增加，小鼠肾组织的体外发育呈下降趋势。

At the end of the culture, PBMCs cultured on coverslips were fixed and stained for tartrate resistant acid phosphatase, a marker of osteoclast.

对培养终末细胞作组织化学染色，检测破骨细胞特征性标志物抗酒石酸磷酸酶的表达；并以象牙磨片上虫蚀样骨吸收陷窝的形成为指标检测其生物学活性。

After 3-8 passages, the oral CAFs and the normal mucosa fibroblasts from tissue culture were observed under light microscope. Vimentin,α-SMA and Cytokeratin protein were detected by immunohistochemistry SP method on glass coverslips. VEGF, TGF-β1, MMP-9, Tenascin-C mRNA were analyzed by semi-quantitative RT-PCR.

用组织块培养法获得原代CAFs和正常粘膜成纤维细胞，0.25%胰蛋白酶消化传代和纯化细胞；通过形态学观察和免疫细胞化学染色对口腔CAFs和NMFs进行鉴定：采用半定量RT-PCR检测口腔CAFs和NMFs 4种基因的表达。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。