组织培养

Key words: Schwann cell; Wallerian degeneration; artificial nerve; tissue engineering

10 d龄SD大鼠坐骨神经进行结扎预变性，分别于术后24 h、3 d、5 d、7 d和9 d，进行雪旺细胞培养和S-100免疫组织化学染色观察。

Osteoblasts isolated from the cranium of Zelanian rabbit,after being transfected with green fluorescent protein,were inoculated onto the bio-derived bone to investigate the 3D fabrication of engineering bone in rotating wall vessel bioreactor.

用经绿色荧光蛋白标记的新西兰兔颅骨来源的成骨细胞复合到生物衍生骨支架材料上，在新型旋转壁式生物反应器内三维构建组织工程骨，同时与静态培养环境中构建的结果进行比较。

Viciaefolia Scop had been cultured and produced calli, and 〓lantlet were regenereded from protocalli of O.

对3个品种(O.viciaefolia)的原生质体进行了培养均得到了愈伤组织，并得到了埃斯基var。

This major aims to train compound professionals which possess fine professional ethics and knowledge, and meet the requirements of corporations or non-profit organizations.

本专业旨在培养满足企业和非盈利组织需要、具有良好的职业道德和专业知识、掌握基本专业技能、能成为会计师或注册会计师的复合型专门人才。

Objective: To explore in vitro culture of fibroblasts companying human hepatoma and observe the cell growth.

目的：体外培养人肝癌组织伴生成纤维细胞，观察其生长状态，为进一步研究其功能奠定基础。

There is no difference in the teaching of vocal music in teachers colleges and conservatories of music over a long period of time.

长期以来，高师声乐教学与音乐院校没有区别，这不利于学生理论知识的学习和组织能力的培养。

Four part: Bring up the ways on the cultivation of Chinese ecological culture qualities, including the counterplots about education, government, company and non-government organization, and the ways to cultivate the scientific education circles, all levels of government leaders, women and the youth.

第四部分：提出中国公众生态文化素质养成的对策和路径，包括教育、政府、企业、非政府组织四个方面的对策，科技教育界、各级政府领导者、妇女、青少年四个公众群组的培养途径。

The bioreactors are widely used in the cell culturing in tissue engineering.

生物反应器用于组织工程细胞的培养目前已经应用的比较广泛。

And try to find out the effect of TSA on the proliferation and differentiation of rat tracheal stem cell. Methods 1.Preparation of 5-Fu Model and TSA treatment Two weeks" Wistar rats provided by the animal center of the China Medical University. The animals were sacrificed by 10% Chloral Hydrate 0.4ml/100g. Then the tracheas were excised aseptically. Then they were douched with PBS and cultured in 1:1 mix of Dulbecco"s modified Eagle"s medium and Ham"s F-12 medium (DMEM/F12) containing 120 mg/ml 5-Fu and 10% fetal bovine serum for 12h at 37°C. The tissues were then refreshed by culturing in DMEM/F12 containing 10% FBS and 200nM TSA.

1、制备气管损伤模型及剥离上皮取约200克左右的Wistar大鼠，雌雄不限(中国医科大学实验动物中心提供)，腹腔注射10%水合氯醛0.4ml/100g，无菌条件下取出气管，无菌PBS反复冲洗，洗净血液和粘液，置于DMEM/F12培养液中(含10%胎牛血清)，于培养液中加入终浓度为100mg/ml的5-Fu，37℃，5%CO2孵育12小时，弃去上述培养液，换成新鲜DMEM/F12液(含10%胎牛血清)，同时加入终浓度为200nM的TSA继续培养，于换液后3、6、12、24、48小时分别10%中性福尔马林固定，石蜡包埋，制成4μm厚的组织切片。

The adjacent region in 3mm diameter was eradiated in various ways: exposed directly to light, through AcrySof one piece IOL, PMMA IOL,AcrySof Natural IOL, and without light. Then the eye cups were cultured for 48h. Lastly, tissue and cell structure were observed with light microscope and transmission electron microscope.

在无菌条件下将新鲜离体猪眼制成眼杯，利用显微镜光源对视网膜后极部照射，使相应视网膜蓝光光照强度为30J/cm2或40J/cm2，进行单独光照、透过Acrysof一片I0L、透过Acrysof Natu-ral，照射后培养48h，对相应部位视网膜组织，HE染色光镜、透射电镜下观察。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。