组织培养

What we called the motif education is that according to the requirement of development of society and modern education,the pedagogue,through enlighening and channeling out the interior requirement for education to the educatee make harmonious ,loose and democratic enviroment of education,purposfuly,planfully organizing and making out various of educating movement ,so that cultivating them to be the main body of society through independently,motilely and creatively realizing and practicing activities.

所谓主体性教育，就是根据社会发展的需要和教育现代化的要求，教育者通过启发、引导受教育者内在的教育需求，创设和谐、宽松、民主的教育环境，有目的、有计划地组织、规范各种教育活动，从而把他们培养成为自主地、能动地、创造性地认识和实践活动的社会主体。练习翻译？遇到难题？快来翻吧

Transformed roots were inocuiated with resting spores of Plasmodiophora brassicae. On solid MS medium, the resting spores infected root haires but not root cortical tissues, and therefore no syndrome of club robt disease was found.

用根肿菌休眠孢子接种离体培养的转化根，结果发现，在固体培养基上，根肿菌只侵入根毛内，不侵入根皮层组织，因而不表现根肿病的任何症状。

Therefore, the objective of this research is to cultivate the plant tissue cells which can secrete an anticancer composition – podophyllotoxin .

针对於此，本实验室建立以八角莲为主题之植物组织细胞培养系统，特别是针对其抗癌成分-鬼臼毒素来进行研究。

Orexin A(100 and 1000 nM) stimulated the release of GnRH from hypothalamic explants harvested from prepuberal swine.However,only 1000 nM orexin B could stimulated the release of GnRH from the hypothalamic explants harvested from prepuberal swine.

五、Orexin对性成熟青年母猪下丘脑组织-垂体-卵巢轴作用的体外研究为探讨orexin在生殖调节中直接作用的靶位点，我们在体外培养了猪下丘脑脑片、垂体和卵巢细胞，研究了orexin A和orexin B在体外对下丘脑、腺垂体和卵巢GnRH、LH和E2释放的影响。

Methods A clinical infection fungus strain was isolaled from mouth pseudomembrane vaginal,nasal secretions,urine,faces,and live puncture tissue,injured skin tissue of a female patient,who had no clear underlying disease.

方法从 1名未确诊女性患者受损皮肤，皮痂，口腔伪膜，阴道及口腔分泌物，尿便及肝穿刺组织，分离与培养出一种真菌，经生化分析，菌种鉴定分子生物学实验及DNA测序。

Methods:Cardiac muscle of twoday neonate rattus was digested repeatedly by 0.0625% trypsin.The cardiocytes were collected in DMEM medium,which contains 10% fetal bovine serum.Myocardial cells were isolated with the technique of differential anchoring velocity with 90 min and were pured by Brdu.The cardiocytes were placed into CO2 incubator to incubate seven days after their purification.

应用0.0625%的胰蛋白酶重复消化出生第2 d乳鼠的心肌组织多次，收集的细胞用含10%胎牛血清的DMEM培养基中和，用差速贴壁分离法分离，在以溴脱氧尿嘧啶纯化心肌细胞后置CO2培养箱孵育7 d。

The results had positive cultures of the pyloric spiroid bacteria in 59 of 98 samples.

结果：98份样本中，培养出幽门螺旋菌59例，其中慢性胃炎阳性率62.7％(浅表性胃炎阳性率最高，达91.1％)，消化性溃疡阳性率57.4％；在慢性胃炎患者胃窦粘膜病理切片中可检出，仍以慢性浅表性胃炎检出率最高达91.1％。15例幽门螺旋菌阳性消化性的溃疡患者，经TDB治疗4周后有12例细菌消失，并伴有组织形态学改变。

Using New Zealand rabbit ears cartilage makes chondrocyte suspension, Fibrinogen was mixed with D-hank's makes a final fibrinogen concentration of 50mg/ml mixture solution, then chondrocytes resuspend with fibrinogen solution, chondrocytes-seeded fibrinogen was mixed with thrombin (25U/ml in 40mM calcium choride) to make chondrocytes/fibrin glue polymer. Pluronic F-127 was mixed with D-hank's makes a final Pluronic F-127 concentration of 400mg/ml mixture solution, then chondrocytes resuspend with Pluronic F-127 solution to make chondrocytes/Pluronic F-127 polymer. The chondrocyte concentrations was 10 million chondrocyte/ml of polymer.

为确定纤维蛋白凝胶与Pluronic F-127在注射方式形成组织工程化软骨过程中的优劣，我们进行了如下实验：应用新西兰大白兔耳软骨获取软骨细胞并体外培养，纤维蛋白原应用D-hank's液配制为50mg/ml，然后以纤维蛋白原溶液重悬软骨细胞，再与40mM的氯化钙配制的25U/ml凝血酶混合形成软骨细胞—纤维蛋白凝胶复合物；Pluronic F-127应用D-hank's液配制为400mg/ml，同样用PluronicF-127溶液重悬软骨细胞而形成软骨细胞—Pluronic F-127凝胶复合物。

Methods Pregnant rats were gavaged castor oil-diet in 18 and 19 days of gestation. At the time of death, blood of portal and peripheral veins, intestinal mucosa, amnion, amniotic cells and placenta were collected, and the tissues were cultured in the presences of ricinoleic acid or indomethacin.

将妊娠18天的Wistar大鼠分实验组和对照组各8只，实验组大鼠每天两次灌服引产餐，灌服4次后剖杀大鼠，收集门静脉、末梢血，胎盘、羊膜组织及制备羊膜细胞进行体外培养。

METHODSUsing the MTT assay, transmission electric microscope, DNALadder method, TUNEL method and immunocytochemistry, the inducing APO and antiproliferation of the extract of Rubia Cordifolia L for MGC803 cell line of gastric carcinoma were studied in morphology, biochemistry and immunocytochemistry.

方法］利用组织细胞培养技术及MTT法，通过电泳分析及多种细胞染色等方法，观察茜草提取物对胃癌MGC803细胞株的凋亡及抗增殖情况。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐