组织培养

Results Astrocyte of rat optic nerve was cultured and purified,which showed positive reaction to glial fibrillary acidic protein.

组织块原代培养，振荡法分离纯化视神经的星形胶质细胞，对细胞的损伤小、纯度高，是快速得到该细胞的理想途径。

The genotypical differences of anther-culture ability and its inheritance were investigated with seven keng rice Oryza saliva sub sp.

kcng品种按双列杂交方式配成的21个杂交组合进行花药培养，测定它们的愈伤组织诱导率和绿苗分化率。

Tissue culture isolation is an effective isolation of human synoviocytes to establish the human synoviocyte line of primary gonarthritis.

结论组织块培养法可以有效分离人类关节滑膜细胞，建立原发性膝骨关节炎患者滑膜细胞系。

Objective To research the effects of different culture and different exophyte on the callus of Halenia elliptica D.

目的探讨不同培养条件及不同的外殖体对椭圆叶花锚愈伤组织诱导的影响。

the seeds of wheat and corn were dipped in water-soluble substance distilled from peganum harmala, before incubate in greenhouse. The moisture of leave tissues was determined at seedling period.

小麦、玉米种子经骆驼蓬提取物水溶液浸泡处理后，室内培养，在幼苗期测定叶片组织的水分状况。

In this study, Mono-nuclear cells containing hematopoietic stem/progenitor cells were isolated from umbilical cord blood of normal full-term deliveries, healthy adult and heterocellular HPFH patient bone marrow by density gradient centrifugation. After simple purification and expansion in vitro, MNCs were induced to erythroid cell differentiation using different culture systems and cytokines including Epo, IL-3 and GM-CSF.

本研究首先利用密度梯度离心方法，从正常成人和异细胞型遗传性胎儿血红蛋白持续存在综合症家系患者的骨髓组织、健康足月正常分娩胎儿脐带血中分离得到了含有造血干／祖细胞的单个核细胞(Mono-Nuclear Cell，MNC)，进行初步纯化及体外扩增后，组合使用Epo、IL-3、GM-CSF等细胞因子，将造血干／祖细胞向红系方向进行诱导培养。

Objective To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique.

目的探讨应用改良Percoll非连续密度梯度离心法分离纯化培养组织工程用肌卫星细胞。

Methods:The subcutaneous adipose tissue was obtained from the fold inguen of four SD rats, then digested with collagenase type Ⅰ and cultured in endothelial medium for seven days.

获取4只雄性SD大鼠鼠蹊部皮下脂肪，通过机械分割和组织消化法获取脂肪基质细胞，在内皮细胞培养液中培养7d后，经免疫细胞化学及透射电镜鉴定证实95%以上细胞已具内皮细胞表型特征，再将这些细胞进行成脂(DMEM/F-12培养基内加入0.5mmol/L1-甲基-3-异丁基-黄嘌呤，1μmol/L地塞米松，10μmol/L胰岛素，200μmol/L吲哚美星)定向诱导7d，形态学观察和油红O特殊染色鉴定诱导后的细胞。

Using suitable and effective inspiriting mechanism.(4)Effective empowering.(4) Cultivating team spirit.

主要是采用建立健全产品研发项目经理人机制、进行合适的项目组织规划与设计、采取合适和有效的激励机制、有效授权和培养团队精神等措施。

The results showed:(1) MP polymerase chain reaction test showed positive results in the bronchoalveolar lavage fluid from all of the MP-infected rats (infectious group, n=4), while they were all negative in BALF from the control group (n=4,P＜0.05) and from those rats both infected with MP and, at the same time, treated with erythromycin (treating group n=4,P＜0.05). Bacterial cultures of the bronchial and lung tissue were negative in all three groups. Using transmission electron microscope the widened interalveolar septa with increased amount of collagen were found in the infectious group, while there were no obvious abnormalities in the other two groups.

结果显示：(1)感染组动物(n=4)支气管肺泡灌洗液肺炎支原体-PCR检测均为阳性，而对照组(n=4)和感染加红霉素治疗组动物(n=4)均为阴性(P＜0.05)；三组动物的支气管和肺组织常规细菌培养结果均为阴性；感染组动物透射电镜检查见肺泡间隔增宽，其中有较多胶原纤维堆积，其余两组则未见明显异常。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐