组织培养

Mouse embryonic stem cells can give rise to ectodermal derivatives in culture and can be further induced into neurons and glia for cell-replacement therapies in the central nervous system and for use in drug discovery.

小鼠胚胎干细胞定向分化为神经细胞的研究进展论文小鼠胚胎干细胞（embryonic stem cell ， ES cell）体外培养可以分化成为外胚层组织，并进一步分化为神经元和神经胶质细胞。

Mouse embryonic stem cells can give rise to ectodermal derivatives in culture and can be further induced into neurons and glia for cell-replacement therapies in the central nervous system and for use in drug discovery.

来源：中国论文服务下载 [ 07-06-18 17：33：00 ] 作者：陆建峰，宋后燕编辑：king 小鼠胚胎干细胞（embryonic stem cell ， ES cell）体外培养可以分化成为外胚层组织，并进一步分化为神经元和神经胶质细胞。

The cotyledon cubes as explants of oil tea were cultured on MS medium added with 2,4-D 2.0mgL^(-1) and KT 1.0 mgL^(-1) to form embryogenetic callus.

以油茶子叶块为外植体，在附加2.0mgL^(-1)2，4-D和1.0 mgL^(-1)KT的MS培养基上进行培养，观察诱导产生的体细胞胚性愈伤组织细胞学结构。

In this study, some transgenic cotton plants with a phytase gene were obtained successfully via agrobacterium-mediated method of embryogenic calli.

本研究采用农杆菌介导的方法，将外源植酸酶基因导入棉花品种的胚性愈伤组织中，经培养获得再生植株。

A virus strain with high hemagglutinin titer was isolated out from embryonated egg and MDCK cells after they were inoculated with the lung tissues of the died marmosets.

在排除细菌感染的基础上，通过死亡狨猴肺组织匀浆接种鸡胚和MDCK细胞的分离培养，分离出一株具有很高血凝效价的病毒株。

A virus strain with high hemagglutinin titer was isolated out from embryonated eggs and MDCK cells after inoculating with the lung tissues suspension of the dead marmosets.

在排除细菌感染的基础上，通过死亡狨猴肺组织匀浆接种鸡胚和MDCK细胞的分离培养，分离出一株具有高血凝效价的病毒株。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

HPF used were primary cell culture with exairesis human pterygium tissues.

利用手术切除的人翼状胬肉组织进行原代细胞培养，即得到HPF。

The inducing rate was 94.5%; the optimal medium for the inducting rate of fasciculated shoot was MS+1.0mg/L6-BA+0.5mg/LNAA+stem section with bud, The inducing rate was 37.5%.

诱导愈伤组织的最佳组合为MS+1.0mg/L BA+1.0mg/L NAA+0.5mg/L 2，4-D+叶片，其诱导率为94.5%；诱导丛生芽的最佳组合为MS+1.0mg/L 6-BA+0.5mg/L NAA+带芽茎段，其诱导率为37.5%，增殖培养中，以MS+1.0mg/L BA+0.05mg/L NAA效果最好，增殖率为460%，当株高在2cm以上时，转入1/2MS+0.8mg/L IBA+0.2mg/L NAA生根培养基上，接种30d后，生根率可达90%，移栽成活率达92%。

The frequency of callus induction was different when the same kind of Festuca arundinacea seeds was cultivated in MS medium supplemented w ith different types of and different concentrations of hormones.

通过愈伤组织的继代和分化培养，诱导出丛生芽，分化培养基以MS + 4mg L 2 ，4 D + 0 。2mg LKT为最佳，初步建立起高羊茅的植株再生体系

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐