组织培养

The biological polymorphism of Cercospora zeae-maydis was studied systematically in the levels of histology, protein and DNA. The major characters in polymorphism of pathogen were listed as follows:(1) The great differences were seen in shapes and sizes of conidiophores, the growth velocity of mycelium and pigment-productivity of colony among pathogen strains. In addition, an albino strain, a kind of mutant, was casually discovered during isolation of pathogen from diseased plants, which supported strongly our previous prediction that there should be some potential genetic variation in Cercospora zeae-maydis.

本研究在组织学、蛋白质和DNA水平上，系统研究了玉米灰斑病菌的生物学多态性，这种多态性主要表现在以下几个方面：①病菌形态学和培养性状多态性：菌株间存在分生孢子形态、菌丝生长速度、菌落产色素能力等方面的差异，该病菌在自然界存在另一种变异类型——白化菌株，说明灰斑病菌的确存在遗传变异的潜能。

OBJECTIVE: After purification and amplification in vitro, the bone marrow mesenchymal stem cells were induced into chondrocytes, and then implanted them at the bottom of nano-hydroxyapatie/chitosan scaffold for combined culture. The aim was to investigate the feasibility of them used in tissue-engineered chondral composite graft.

目的：将体外提纯、扩增的骨髓间充质干细胞诱导成软骨细胞，将其接种于穿&靴&的纳米羟基磷灰石/壳聚糖支架的底部上联合培养，探索其用于组织工程软骨复合体的可行性。

Objective: To examine the property and proliferation ability of primary culturing MSC, investgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

目的：了解骨髓基质干细胞原代培养的特性及增殖能力，探讨轻度离心和化学限定培养基法诱导骨髓基质干细胞向成软骨方向分化的作用；为软骨组织工程提供足量、合适的种子细胞。

This experiment approach rat-MSCs which are fractional cultivation、generation in vitro, and in Chinese crude drug circumstance to derivationrat-MSCs chondroblast differentiated, to explore the front view ofcartilage coloboma architecture engineering recovery by Chinese crudedrug.

本实验探讨大鼠骨髓间充质干细胞在体外分离培养、增殖，以及在中药环境下诱导向成软骨细胞定向分化，探索软骨缺损组织工程修复中的中药应用前景。

Methods. Cells of the nucleus pulposus and inner and outer anulus fibrosus were enzymatically isolated from boine coccygeal tissue and precultured in alginate beads for 14 days.

髓核细胞和内外纤维环通过酶与牛尾骨组织隔绝并在14天前培养于藻酸盐中。

Through clinical symptom ,pathological examination ,histopat hology ,germcult ure ,biochemical experiment s and drugsensitive test ,we diagnosed that it was colibacillosis and endenic.

经过临床症状、病理剖检、组织病理学、细菌培养、生化试验、药敏试验等诊断方法，确诊为鸡的大肠杆菌病，并为地方流行病。

Objectives: Produce academic work Offer courses in the field of law and technology Foster interdisciplinary contacts with other faculties at Dalhousie and elsewhere Produce the Canadian Journal of Law and Technology, a peer-reviewed national online journal providing coverage of legal issues relating to information technology from a Canadian and international perspective Organize seminars and colloquia.

信息与技术研究机构的主要活动包括：创作学术作品，提供信息与技术方面的课程，培养跨学科之间的联系与合作，制作加拿大信息技术与法专刊，这个刊物是在线的，主要是以加拿大实际与国际视野相结合，提供与信息技术相关的法律问题的报道，组织学术会议和专题座谈会。

The objective of this study is to investigate the localization of the conjunctival stem cells in adults.

进一步研究结膜干细胞的确切定位及生物学特性、体外分离培养结膜干细胞、用组织工程构建生物结膜是结膜干细胞的研究方向。

METHODS: In september 1999, human embryonic tendon cells and artificial materials were co-cultured in vitro to construct tissue engineered tendon, which repaired coracoclavicular ligament injury.

1999年9月采用人胚肌腱细胞与材料体外复合培养，构建组织工程化肌腱，修复喙锁韧带损伤。

AIM: To establish the methods for rat costochondral growth plate chondrocyte separation and culture and investigate their biological features, thereby provide experimental bases for studying the regulation of chondrocyte proliferation and differentiation.

目的：建立大鼠肋生长板软骨细胞分离、培养的方法，探讨其生物学特性，为软骨细胞增殖分化的调控和软骨组织工程修复的研究建立实验基础。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐