组织培养

DC.,which included sterilization of explants,the induction of callus and establishment of cell suspension culture system of Ardisia pusilla A.DC.,the examination on the process from the seed germination to mature plant,the discovery of "multi-embryo" phenomenon.

内容涉及：外植体消毒方法确定，愈伤组织诱导和悬浮培养，九节龙培养基硬度最适条件的确定，九节龙从种子萌发到成苗过程的观察，九节龙种子的&多胚现象&的发现。

The results demonstrated that the strain isolated from fruitbody tissue of Armillaria luteo-virens was the true pure culture of the fungus.

结果表明，本研究获得的黄绿蜜环菌子实体组织分离菌株即为其纯培养菌种。

Particles of different material have different effects on synoviocytes. This appearance has some similarity with the reaction in the interface membrane around aseptically loosened prostheses.

不同的人工关节材料颗粒对体外培养滑膜细胞的作用不同，其表现与之在松动人工关节界膜组织中的反应相似。

Today, ASSE organizes international exchanges for high school students in 21 countries.

今天，ASSE为21个国家的中学生组织国际效交换培养。

There was apparent interspace between repaired tissue and surrounding normal tissues. The results indicated that: 1 Although autogenic cartilage obtained from patella in knee joint is limited, chondrocytes cultured in vitro could be regulated by some growth factors, therefore sufficieng and well-phenotype chondrocytes could be harvested to construct tissue engineering implants for transplantation. 2 It showed well therapeutic efficacy with the method of repair in our research.

实验结果表明：①自体膝关节髌骨来源的软骨虽有限，但在体外单层培养时应用生长因子对其进行调控，能为自体关节软骨缺损的移植治疗提供数量充足的、表型良好的软骨细胞以用于构建组织工程移植物；②本实验中所应用的修复方法治疗效果良好，能较有效地克服和解决目前在临床上所用的自体关节软骨细胞移植修复关节软骨缺损时所存在的软骨细胞容易丢失等问题。

Furthermore, cell culture and amplification are comparatively easy so we can get a great quantity of cells applied by autografting and allografting.

目的：探讨由脂肪组织分离获得脂肪基质细胞的方法及其在体外经诱导培养后向成骨细胞分化的潜能。

Totally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT.TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells.

对北京协和医院根据细菌学或／和组织学诊断的肺外结核患者31例，应用酶联免疫斑点技术检测6kD早期分泌靶向抗原和10kD培养滤过蛋白肽段库刺激后外周血中释放γ-干扰素的特异性T细胞。

Methods The skeletal muscle stem cells were enzymatically isolated with collagen and dispase, and then purified by different adhesion time from 6 female SD rats. The purified cells were transinfected with adenovirus carrying lac-Z gene, and injected into the bladder neck of 6 autologous and 4 allogeneic rats. Tissues of blader neck and ureter were obtained in sacrified rats after 5 and 15 days, histological examination and X-gal staining were performed.

采用混合酶消化及反复差速贴壁法分离、纯化培养SD大鼠骨骼肌干细胞，然后用携带lac-Z基因的腺病毒载体转染肌干细胞，待转染成功后将携带lac-Z基因的骨骼肌干细胞注射于自体和同种异体的膀胱颈及后尿道周围，分别于注射后5、15d，处死大鼠取出膀胱颈及后尿道，行组织学检查及X-gal染色。

It was found that the extract of Ficus carica leaves showed remarkable inhibitory and killing effects on the Newcastle disease virus.

采用组织细胞培养技术，充分利用无花果叶提取物中的有效成分，在人上皮样癌（Hep-2）细胞株、地鼠肾（BHK21）细胞株和鸡胚成纤维细胞上研究其抗新城疫病毒的作用。

The soak test was made for Eremochloa ophiuroides, Potamogeton crispus L and Ceratophyllum demersum selecting from common submerged plant that planted in barrel indoor. The contaminants release rule induced by dissolution of plant's tissue was inspected. The result shows that the quickest release happened within the first 8 hours.

选取在室内水缸中培养的常见沉水植物——蜈蚣草、菹草和金鱼藻进行浸泡试验，考察植物类型对因植物组织溶解而导致的污染物释放的影响。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐