组织培养

Methods OECs were cultured in the olfactory bulb of newborn wistar rat. OECs suspension was infused into the lateral cerebral ventriculus of AE rats, intraperitoneal methyl-vitamine B12 associated with above OECs suspension in combined transpiantion group, and the saline with the same quantity was infused in pseudo-operation group. Afterwards, sport-function grade, histo pathological change were observed.

用新生wistar大鼠嗅球培养OEC，移植组将OECs悬液注入实验性变态反应性脑脊髓炎大鼠的侧脑室；联合移植组在侧脑室注入OECs的基础上，腹腔注入甲基维生素B12；假手术组按以上方法注入等量生理盐水；观察两组大鼠运动功能评分、组织病理学变化。

In this study, the expression and function of FGF-13 in developmental follicles and skin during embryonic development,normal vibrissa follilce stem cells and wound follicles in vitro were obserbed,so as to illuminate the possible role of FGF-13 in the skin follicles during embryonic development and damage healing and provide the experimental base for further studying the characteristic and mechanism of the role of FGF-13 in skin.

采用免疫组织化学(Immunohistochemistry，IHC)、免疫细胞化学(Immunocytochemistry， ICC)和原位杂交(in situ hybridization，ISH)技术及体外大鼠触须毛囊的器官培养，研究了三种不同的生物学过程中FGF-13在皮肤毛囊中的表达及作用。

Objective To setup a new technique of tissue and cell culture for vitreous aspirates.

目的 建立玻璃体切除液组织和细胞培养的新技术。

I believe I will be a good member of your company with my well-knit major knowledge, and my comprehensive ability.

在社会实践中，因为渴望成为一名具有双语交际能力的综合人才，我一直积极参加社会实践活动，在公司兼职期间我学会了团队精神的可贵，组织能力和人际交往也有了很大的提高，并学会注意生活中的小细节；在各类英语培训学校兼职期间培养了我的教学能力，细致谨慎的性情以及师生间良好的沟通互动技能。

But the chondrocytes lost primary characteristics after several passages.Conclusion A great of chondrocytes can be got from xiphoid, and the chondrocytes can grow in suspension and keep primary characteristics for a long time when they are cultured with polyHEMA in vitro. In this way, good seed cells are provided for experiment in vitro, and for the study of tissue engineering, and for clinical study.

从剑突可获得大量软骨细胞，使用PolyHEMA试剂体外培养的软骨细胞，其软骨细胞能长期以悬浮状态生长，并能使软骨细胞长期保持原有的生物学特性，为体外实验及组织工程学的研究提供更优质种子细胞，为临床的进一步研究提供理论基础。

Calluses were induced from hypocotyl segments of seedlings of Alhagi pseudalhagi on MS medium supplemented with 1. 5-2. 0mg/L 2, 4-D and 0.5-1. 0mg/L 6-BA.

骆驼刺种子无菌苗下胚轴切段在含1.5-2.0mg／L2，4-D和0.5-1.0mg/L6-BA的MS培养基中可100％诱导产生愈伤组织，并在相同培养基上进行继代培养。

METHODS: From January 2000 to May 2002, 52 cases with various types of bone defect were treated with tissue engineered bone, which was constructed in vitro by allogeneous osteoblasts from periosteum 1 x 10(6/ml with bio-derived bone scaffold following 3 to 7 days co-culture. Among them, there were 7 cases of bone cyst, 22 cases of non-union or malunion of old fracture, 15 cases of fresh comminuted fracture of bone defect, 4 cases of spinal fracture and posterior route spinal fusion, 3 cases of bone implant of alveolar bone, 1 case of fusion of tarsotarsal joint.

2000年1月～ 2002年1月，用同种异体骨膜来源的成骨细胞 1×106 / ml与生物衍生骨支架材料构建的组织工程骨，经体外培养 3～ 7天后，植入修复52例各种类型的骨缺损，其中骨囊性病变7例，陈旧性骨折不愈合或畸形愈合 2 2例，新鲜粉碎性骨折伴骨缺损 15例，脊柱骨折后路植骨融合术4例，牙槽骨植骨 3例，足跗中关节植骨融合 1例。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

The system was able to help androgone in testis tissue go through meiosis in vitro without addition of testos-terone by providing necessary conditions for differentiation of androgone.Key words: testis;tissue culture;in vitro spermatogenesis;transmission electron microscopy;HE staining

证实，在没有添加睾酮的情况下，此培养体系能够提供睾丸组织块生精细胞分化所需要的条件，使非精子细胞类生精细胞减数分裂为精子细胞。

Objective］ The study aimed to explore the protoplast isolation and culture of callus in Apocynum venetum and the influence factors.

［目的］探究罗布麻愈伤组织原生质体的分离培养及其影响因素。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐