组织培养

This thesis conclude 4 parts: 1 、 The change and effecting factors of plasma adiponectin level in old subjects with abnormal glucose tolerance; 2、 The distribution of adiponectin receptors in Wistar rat; 3、 The effect of fat-fed and pioglitazone on mRNA expression of adiponectin with its receptors...

本研究共包括以下四部分内容：1、老年糖耐量异常人群中脂联素的改变及其影响因素；2、脂联素受体mRNA在Wistar大鼠体内的分布；3、高脂饲养以及吡格列酮对大鼠脂肪组织脂联素及其受体表达的影响；4、吡格列酮对不同浓度脂肪酸培养的HepG2细胞脂联素受体mRNA表达的影响。

Methods:Pigspleen cells were cultured with HBvaccine and phytohaemagglutinin in which the sensitized cells were collected and homogenized to pieces by a constitution triturator;the homogenate was diluted with distilled water to 1∶1, then frozen and melt six times at25 ℃ before placed in the dialyzer at the melting state , after that dialyzed with 1∶4 distilled water as the outdialyzate at 4 ℃ for 48 h;the final outdialyzate was collected as the HBVSTF.

采用猪脾细胞与植物血凝素、乙肝疫苗共同培养，收集致敏细胞在组织捣碎机中破碎，用蒸馏水1∶1稀释，－25 ℃反复冻融6次，自来水融化后装入透析袋中，用1∶4的蒸馏水作透析外液，于4 ℃透析48 h，透析液即为乙肝病毒特异性转移因子。

Methods: Pig-spleen cells were cultured with HB-vaccine and phytohaemagglutinin in which the sensitized cells were collected and homogenized to pieces by a constitution triturator; the homogenate was diluted with distilled water to 1:1, then frozen and melt six times at -25℃ before placed in the dialyzer at the melting state, after that dialyzed with 1:4 distilled water as the out-dialyzate at 4℃ for 48 h; the final out-dialyzate was collected as the HBV-STF.

方法；采用猪脾细胞与植物血凝素、乙肝疫苗共同培养，收集致敏细胞在组织捣碎机中破碎，用蒸馏水1：1稀释，-25℃反复冻融6次，自来水融化后装入透析袋中，用1：4的蒸馏水作透析外液，于4℃透析48h，透析液即为乙肝病毒特异性转移因子。

This two-track parallel form of organization not only carry forward the teaching of the team teaching system, teaching system, mass culture, a large-scale advantages of improving the quality of teaching and its abilities to overcome the shortage of fully taking into account each child's individual needs and personality.

这种双轨并行的教学组织形式既发扬了班组授课制系统讲授、成批培养、大规模提高教学质量的优势，又克服了其在因材施教上的不足，充分照顾到了每个儿童的个体需要和个性发展。

Facing opportunities and challenges,the company will constantly improve its organization and management,encourage technology upgrade,and foster a culture f ull of innovation and teamwork.

面对机遇和挑战，公司不断完善组织结构及管理模式，鼓励和推动技术进步，培养锻炼富有创造性和团队精神的员工队伍。

For the sports industry to go up a step higher, it depends on whether or not the intrinsic patterns of market economy are followed; sports game management must make some breakthroughs in terms of professionalized conception, market awareness as well as specialized organization and management; sports game sponsoring is not simply "buying publicity with money", it is a valuable thing to maximize sponsor benefits; the varieties of product and service provided by the sports fitness and entertainment market in China are limited and undiversified , and the replacement and high style products are launched onto the market behind the time; in terms of market benefit distribution, we should establish a set of mechanisms with clearly defined responsibility, well established power and definitely clarified interest; the cultivation of professionals in the sports industry is related to the future of the sports industry in China.

体育产业要上台阶，取决于是否真正按市场经济的内在规律办事；体育赛事经营必须在职业化观念、市场意识和专业化的组织和管理方式上有所突破；体育赛事赞助并不是单纯的"花钱买名"，达到赞助权益的最大化才是最具有价值的；我国体育健身娱乐市场所提供的产品和服务品种有限且单一，产品换代及新潮产品生产滞后；在市场权益处理方面，需要建立一套责任明晰、权力到位、利益明确的机制；体育产业专门人才的培养，关系到我国体育产业的未来。

The special contingent should be set up in the hospital, the cadreman of the part-time job is trained in the grass roots medical treatment units, the retiree is utilized for building up the unestablished contingent of the community nursing.

医院应组织专门队伍，在基层医疗单位培养兼职骨干，利用退休人员组成编外社区护理队伍。

The nucleus vegetative in nature derived from unequal or equal division of the uninucleate pollen in rye anthers cultured in vitro develops into pollen embryoids or calli.The green haploid plantlets are differentiated successfully.

离体培养黑麦花药，单核花粉可以通过不等分裂和均等分裂由营养性质的核发育成花粉胚状体或愈伤组织，并且最后形成了单倍体植株。

Ring (United Nations Industrial Development Organization Industrial Clusters and capital operation responsible person): I think the financial system in the banks is a little plate, Guangdong's financial development is one of the largest institutional barriers, Guangdong We must develop a innovative systems and ideas.

罗响(联合国工业发展组织产业集群与资本运作负责人)：我觉得银行只是金融系统中极少的一个板块，广东金融发展一个最大的问题就是体制障碍，广东一定要培养起具有创新性的体制和思路。

OECs were cultured in the olfactory bulb of newborn Wistar rat. OECs suspension was infused into the lateral cerebral ventriculus of EAE rats, and the saline with the same quantity was infused in pseudo-operation group. Afterwards, motor-function grade, histo-pathological change and expression of apoptosis of neuron were observed.

培养、纯化OECs后，移植组将OECs悬液注入EAE大鼠的侧脑室；假手术组按以上方法注入等量生理盐水；观察二组大鼠运动功能评分、组织病理学变化以及神经元细胞凋亡表达情况。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐