组织培养

In this report, immunohistochemistry and in situ hybridization techniques were perfected to examine the expression of bFGF, fibroblast growth factor receptor-I and bFGFmRNA of cultured HEP-2 laryngo-tumor cell and 803 gastro-tumor cell and of the solid tumor tissue formed by implanting the cultured cells into subcutis of nude mice, intending to elucidate the correlation between the expression level and the tumor formation.

本研究建立了免疫组化和原位杂交的检测方法，通过对体外培养的HEP-2喉癌细胞和803胃癌细胞和这两株细胞在裸鼠皮下形成的实体瘤组织中bFGF，纤维细胞生长因子受体-1（fibroblast growth factor receptor-1，FGFR-1）和bFGFmRNA的检测试图揭示三者在体外和体内表达以及与肿瘤形成的关系，从而为这方面的研究提供更多的材料。

The same procedure was repeated 5 times until a new cell subline, designated as SW480/M5, was established, whose biological behaviors was investigated both in vivo and in vitro.

采用外科原位接种、组织块细胞培养法从裸鼠体内获得SW480细胞系中具有肝脏转移倾向的亚克隆SW480/M5；体内体外实验验证它与母系细胞在生物学特性上的差异。

Methods Through digesting human skin with collagenase type Ⅱ, cells of human eccrine sweat gland were isolated.

通过酶消化法分离人皮肤汗腺，微量加样器在倒置显微镜屏视下吸取游离的汗腺组织移入Hank平衡盐溶液，经纯化后予以原代培养。

Methods:Using cell culture and immunohistochemical techniques,the peripheral blood specimens from 33 RA patients were designed to test the human T cell lymphotropic virus type Ⅰ related antigens,reverse transcriptase and spontaneous syncytium formation.

采用细胞培养、免疫组织化学技术等对33例RA患者的外周血样本进行人类嗜T淋巴细胞病毒Ⅰ型相关抗原、逆转录酶活性测定及观察自发性合胞体形成。

Objective:To seek culture conditions suitable for the callus growth and taxol formation of Taxus yunnanensis .

中文摘要：目的：寻找适合于云南红豆杉愈伤组织生长和形成紫杉醇的培养条件。

Primary culture and subculture were adopted using lung cells of a female Elaphodus cephalophus' embryo. A new karyotype and a pair of big telocentric chromosomes were found. The diploid has 48 chromosomes, and the karyotype formula is 2M+2ST+42T+XX. C-banding analysis shows that these chromosomes are homologous with those reported relevant chromosomes. This article is the first to report the polymorphism of Elophodus cephalophus B-chromosomes and studies it's transmitted mechanism.

采用原代培养和传代培养法，对1头毛冠鹿的胚肺组织细胞进行观察，发现一种新的核型，其二倍体染色体数目为2n=48，核型公式为2M+2ST+42T+XX，出现1对大的末端着丝粒染色体，C-带显示该染色体与已报道的相关染色体同源，首次提出毛冠鹿B染色体多态，对其传递机制进行了探讨。

We present a report of a diabetic patient with an infection of his left thumb and thenar eminence. Standard cultures of drainage and tissue biopsy were unrevealing.

我们报告了一位糖尿病病人的左手拇指和鱼际发生了感染，标准的细菌培养和组织活检都不能找出病原体。

Since the restoration and reconstruction of Chinese procuratorial institution in 1978, Chinese procuratorial work has been developing flourishingly with the boom of procuratorial theory research, the appearance of numbers of nationwide procuratorial theory research organizations and academic groups, and the formation of a stable prosecution theory research team, which increasingly enables prosecution to become an independent academic discipline.

检察机关恢复重建以来的30年，我国检察事业蓬勃发展，检察理论研究也日趋繁荣，出现了一批全国性的检察理论研究组织和学术团体，培养了一支稳定的检察学理论研究队伍，检察学作为一门独立学科的条件日益成熟。

Diagnosis of RGM infection was made by culturing synovial fluid and periprosthetic tissue specimens.

RGM感染是通过培养滑膜液和人工关节周围组织标本进行诊断的。

Objective: To study the effect of Shuxuetong injection on cerebral tissue and brain microvascular endothelial cell in secreting tissue-type plasminogen activator.

中文摘要：目的：探讨疏血通注射液对在体和体外培养的脑组织微血管内皮细胞(brain microvascular endothelialcell，BMEC)分泌组织型纤溶酶原激活物(tissue plasminogeon activator，tPA)的影响。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐