组织培养

Cyclooxygenase-2-silenced cells were less able to acidify tissue culture medium, a response that could partly be attributed to decreased lactate production or export detected by reduced lactate in the medium.

同时藉由还原培养皿中的乳酸可以得知COX-2被缄默的细胞酸化组织培养皿的能力较低，而这个现象被认为部分地由减少乳酸产量或输出造成。

The aim of this study is to investigate the combined effects of medium composition, size, shape and ventilation of various vessels on the degree of vitrification and proliferation of tissue-cultured statice.

本实验以星花组织培养繁殖的芽体为试验材，探讨同培养基组成与型式、透气各的培养盒，对试管苗玻璃质化与增殖造成的影响。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

In this research, we employed shoot-tips of wild Chinese tulip tree in Mount Emei as explants for tissue culture, and mesured the content changes of super oxygen-anion free radicals O2(superscript -, hydrogen peroxide (H2O2) and malondialdehyde during the secondary culture.

本研究以峨嵋山野生鹅掌楸茎尖芽为外植体进行组织培养，并对其继代培养再生过程中超氧阴离子自由基O2 (上标-、过氧化氢(H2O2)和丙二醛含量的变化进行了测定。

Since Croomia can spread vegetatively via rhizomes in suitable habitats, a strategy seems feasible involving propagation via rhizome segments and tissue culture techniques, followed by cultivating in garden plots, and subsequent reintroduction into their original wild habitat.

对于个体极少的居群，应采用人工分株和组织培养等措施来促进繁殖，将人工培养的植株再回归到其野生地；同时迁地保护时避免居群间的远交衰退。

In order to raise efficiency of planting seedling by artificial spore culture of Athyrium multidentatum, in this article, spore of Athyrium multidentatum was taken as the explants, and used different categories of hormone, NaH2PO4 and AC during the period of tissue culture.

为了提高猴腿蹄盖蕨孢子人工培养成苗效率，以猴腿蹄盖蕨孢子为外植体，通过在组织培养中加入不同种类激素和NaH2PO4及活性炭，探讨了猴腿蹄盖蕨孢子萌发和成苗影响因素。

Tissue culture methods were used to study the autotoxic effects of root exudation on the growth of second-batch seedlings of Paeonia ostii embryo seedlings.

利用组织培养技术研究了不同生长时期的凤丹种胚组培苗根系分泌物抑制二茬培养苗生长的自毒作用。

In view of studying the suitable type of medium, probable way of explant regeneration and suitable condition of culture for tissue culture in Brunfelsia latifolia , an experimentation was established in which stem segments and a series of different medium were employed.

以茎段为材料，采用不同培养基，研究确定适合二色茉莉组织培养的培养基类型，植株再生的可能途径，以及适宜的培养条件。

Xanthium sibiricum has much application value in medicine, pesticide and other fields. It was callus cultivation system, tissue cultivation technology system of Xanthium sibiricum, antifungal activity of callus extract to Botrytis cinerea Pers.

苍耳在医药、农药等多方面有很高的应用价值，本文对苍耳愈伤组织的诱导、组织培养技术体系的建立、愈伤组织提取物对番茄灰霉病菌Botrytis cinerea Pers。

Using immuno-histochemical, tissue cultural and immuno-electron microscopic methods, based on nerve-endocrine-immune net, the dynamic changes of 4 brain-gut peptides and 6 inflammation factors of excised saccalus rotunds of rabbit stimulated by germs, LPS, exotoxin were observed.

本研究以神经-内分泌-免疫网络学说为依据，运用免疫组织化学的方法，结合组织培养技术、免疫电镜技术等，对离体兔圆小囊受到大肠杆菌等4种细菌、内毒素、外毒素等抗原刺激后，其组织内的4种脑肠肽和6种炎症相关因子表达量的动态变化进行了系统观察研究。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。